CAZyme Information: MGYG000003706_00016

Basic Information

• Species: An181 sp002160325
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; An181; An181 sp002160325
• CAZyme ID: MGYG000003706_00016
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1562	MGYG000003706_2|CGC1	172767.11	4.1907

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003706	1667408	MAG	Estonia	Europe

• Gene Location: Start: 2562; End: 7250 Strand: -

Full Sequence      

MKRKWKKLLAGLCVATMGMSMFAPLGIPARAVQAEDRVSGREVLNFNSDWGFYRGDLENA
EDPDFDDSEFAAVTLPHTMQLAKKHCPGANGVYQGVGWYRRYFTLDESYAGKKIQIQFEG
VMTDSDVYLNGEKIYTRNGGYVGFTVDITDKVKVGETNVLALRVSKVDNPNTPPGKPEAS
LDFHYWGGIYRDVNMIVTEKTYVTDVLEADKAGGGGIFLTYPQVSKEEAVVNVKTDVAND
NEESHEVYVKQILEDKGGAAVVEQESEKQTIAAGESYQFAQDLTVENPHLWGIDDPYLYN
LVTEVYEDGVKVDEVETNAGIRTIDYKPDGFYLNGERVYLRGANRHQAYQNVGDAAPNSM
QYRDALQIKQNGFNAVRAAHYPQDPAFLDACDELGIVVIECQPGWQNFTNNDTFYDLTIR
DTREMIRRDRNRPSVVLWEASLNETNTSDQWAKDAISAAREEYPGDQLFLASDYGYHGEL
YDVCYKVQDTQWSDNQDDWVDFDPEMPFFTREWGDWEGSSKALRKDGESAMNTQVYTRER
YLNGDGYSDWGGLDASERIGGYFLWSWDDYARGSNSVTLGSGTVDIDRYEKNGYYWFQSM
LPADNEVYGPMVYISSDYTKESSLTVPVFSNCDSVRLYQNGTLVKEISREEAGADVPNIM
KKGGSPIYDFQLSEFAAGTLKAEGVVDGEVVCTHEVSTPGAAVKFEVEIGDRGVEPEADG
SDLVPVYIKAVDENGTIVPDYDGTVHVEVGGNGSLVGDDIPRIKVQDQTLEKGIGFAFVR
TSMTAGDIQITATADGMEDGVGTVTTKQTDDTFVADGAHEEWTKTEADLEEPEYSYANIA
AGKEVAVSTEQSGNEKAHLTDDDESTRWCASDGSFPQWAEVDLGSAYAISGFQMMWERTG
ASYQYKIEVSNDKVNYETVLDMSANEQVNGALETQMQKAEGRYIRLTVLGCSDGSWASLY
EFKVIPDEEAEEPEPGELIPDSAVSKIEATSSVEGRGADKLRDGITEIGSGWLSESREFP
QSVTVYFEKPQTLLGSRIYWEKDSSWYTYDLEVTKDGQIWEPIIQDLRVGGQHYKPETFA
QPQENVVAARVTIKNVEAGGVYQIGMAEWMLYGYEYQEPEPEPVKEFEYVSDLEWTSAYS
DYAQVTKNAAAYGGNLVLNTPEGRKEFVKGLGADTNSEIVYTLEEGKYSRFESYIGINAN
ASKNGGEAIFRVYGDDKLLYESPVMMRDDNCEFVSVDIEGVKTLKLEAVWSGNTENPEAR
YNTHIDWADAKVYTKTEELSVEVLKYAIELAKKADLTGAIDSVAAAFEKALADAQNLLER
VDAGEFGITQKMVDEAWQNLIKTMQYLSFEKGDKADLEKVIAAAKAIDLKDYLDEGQDAF
KTGLTKAETVLEDGDAMKDEVDQAWKELLKAMSELRLKPDKDALKALIETAQGMSVEGCS
QETASAFKRALANAVSVYEDEQATQEDVSGAMDNLQASVDDVLASVENKKTDKDDKTIGK
VASADRGTSKTVDGATAGKTANTGAQKSAKTGDVANPAAAAVVLVLAAAAGFAAFERKRK
IK

Enzyme Prediction     

No EC number prediction in MGYG000003706_00016.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	34	600	4.1e-104	0.6183510638297872
CBM51	1129	1271	1.6e-32	0.9776119402985075
CBM32	847	963	1.2e-22	0.9112903225806451

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.74e-64	40	572	9	504	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	6.09e-44	97	445	111	450	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	1.08e-41	93	461	63	438	beta-D-glucuronidase; Provisional
pfam08305	NPCBM	1.29e-33	1129	1272	4	135	NPCBM/NEW2 domain. This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins. This domain has also been called the NEW2 domain (Naumoff DG. Phylogenetic analysis of alpha-galactosidases of the GH27 family. Molecular Biology (Engl Transl). (2004)38:388-399.)
PRK09525	lacZ	1.60e-31	96	453	121	477	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANN35851.1	0.0	39	1271	16	1246
AXE81952.1	3.10e-314	40	1082	89	1186
QIH35625.1	7.57e-251	34	950	22	958
QUT49244.1	2.28e-249	39	954	27	960
AUI47864.1	6.22e-245	41	954	28	959

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4YPJ_A	2.44e-82	40	797	6	798	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
7CWD_A	7.21e-78	44	806	4	801	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
6QUB_B	9.86e-71	45	798	11	845	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]
5DMY_A	1.45e-70	45	798	40	874	Beta-galactosidase- construct 33-930 [Bifidobacterium bifidum],5DMY_B Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum],5DMY_C Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum]
6QUB_A	1.49e-70	45	798	34	868	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum],6QUC_A Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUC_B Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUD_A 2-deoxy-galactose reaction intermediate of a Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	9.52e-94	40	792	2	723	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
A7LXS9	6.24e-71	4	801	5	843	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KN75	2.95e-65	39	799	24	788	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
P26257	2.07e-63	41	757	2	706	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
T2KPJ7	1.79e-57	39	756	49	775	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000687	0.998388	0.000259	0.000269	0.000191	0.000169

TMHMM  Annotations     

start	end
7	29
1534	1556