CAZyme Information: MGYG000003706_00508

Basic Information

• Species: An181 sp002160325
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; An181; An181 sp002160325
• CAZyme ID: MGYG000003706_00508
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1662		186431.03	4.2511

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003706	1667408	MAG	Estonia	Europe

• Gene Location: Start: 168; End: 5156 Strand: +

Full Sequence      

MKKRKPLLAAALAAGLVVSAVPAAANDGQGVTNNDADIFKGEEWFDQNGKFEVNREDAHT
SFIGFDKLENVKDPELRKEKETSPFYYSLNGDWKFKLVDSPHERNMEFFKDGYDVSGWDD
IKVPSNWQTEGYDHPKYTDTRLPWEGVEDPRANYGLPEDSPRGVAPTVYNPVGHYKRTFT
TPKNWDGKEIFVSFQGVESAFYVWVNGQKVGYSEDSYTQDEFDISKYLNAPGEENTIAVQ
VYRWSDGSYLEDQDFIRLSGIFRDVFLYAKDKQASIFDFHYTTDLDESYKDATLNVEITL
RNYDENADTKGYRVRHILYDKDGNEVLEKETTDLVFEKSDDGYGQAHVAFAEDVKAPLLW
SAEAPNLYELVTILEDADGNIVETAGCHVGFREVEIVRKGTNTSQILINGAPIMLKGVNR
HETNPGTGRHISEESMIKDIELMKQYNINAVRNSHYPNEPRWYELCDEYGLYMIDEANIE
SHGLNDYIPQSDEQWISACKDRMRTTIERSKVHPCIISWSLGNESYNGATWAELGKLCKE
LDPTRFVHYEGDRDIPEVDVWSRMYRRVDQPDVLDKTINPMGWWGVYGTKPSFQCEYAHA
MGNGIGNLDEYWEMYEKYPNLQGGFIWDWVDQTIEVATPTDKMLTDEGKTNIPVKLKGDL
VEGAQGKGMDGYAEVYNDKSLHISGRTPFTLEAMVKPDTYEAATRENDGSGQVKPEDYNQ
TAPIITKGNDEWSCTESYGLKRLVDGEKDVLEFYIYNQDYDDSNGVYTKVSAQFDTPDDW
GGKWHHIAGTYDGTNLKLYLDGEEVASATDEVGVVGGPNKVGIGADVTFDAQNPNVPATF
KGVIDNVRIYDRALSKEELNNKDREADNNTLLWLDFDETADKTYEKDTYFSFGGDWQDIP
EGNPNNKNFCANGLISADRTVQPELEQVKYIYQNIGIHDYDILNGKIELENKYLFTNLNA
FNASWELLEDGQVIQSGDFTADDLDVAPVDDSLSEIKSYGKKILNVPFEKPELKAGAEYF
VNISFNLKEDTSWAKAGHEVAHGQIKVPFDVPSVGETDAKEIPEIETVETDDTVTVSGKE
KDFTLEFNKKTGTIDSFIYEGKALLEKGPAPDFWRAPTDSDLGFYSGLELDTWRHAGADR
KVESVNVEKSGNNMVTFTVKSTLPTTNESGYEQKFSVYGTGDVKVTSTLNPGEDLPMIPV
VGNTLTLPKEFSHVTWYGKGPDENYIDRQSGYEVGVYKKDVEDFFVDYIKPQETGNRTDT
RWVSITNDDGVGLLAKAETDTPIEFSALYYTAEDLSNALHSYQLEDKDNVILRLNQRQMG
VGGDNSWGAKPFEKYQNTADHTYEYTYTFKPVTTDEIEASMDEYKVVLPNEGYEGGDEET
EQLSTDVLEYAIELAKTADIENVVGSVAEKFETALANAEDIYAKAKAGDKTIDQEMIDES
WKELIEIMQYLSFKQGDKTDLAKVIALAETMEAKIESYLDGNEKDAFVSALDEARKVNND
KDAMQDDVNIAWKNLLLAMANMQLKPDKDVLEGLVIQAEGFEAADYEAESFETMRAALAE
AKGVLADEKAVQDTVNASCDALETAIAKLTPVKDTNVANAKIPETAENSDKEAGIKEGQT
GTKDPKQTSEKSAKTGDKTTGTAAAVALAAAAAAFAAWRKRK

Enzyme Prediction     

No EC number prediction in MGYG000003706_00508.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	71	655	5.9e-158	0.5997340425531915
GH2	895	1231	2.6e-63	0.37101063829787234

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK09525	lacZ	0.0	36	634	9	576	beta-galactosidase.
PRK10340	ebgA	2.04e-177	54	635	13	551	cryptic beta-D-galactosidase subunit alpha; Reviewed
COG3250	LacZ	5.87e-131	76	635	2	505	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
smart01038	Bgal_small_N	3.88e-96	1081	1349	3	272	Beta galactosidase small chain. This domain comprises the small chain of dimeric beta-galactosidases EC:3.2.1.23. This domain is also found in single chain beta-galactosidase.
pfam02836	Glyco_hydro_2_C	1.06e-87	404	636	6	249	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEE90937.1	0.0	42	1353	32	1254
CDI40488.1	0.0	42	1353	32	1254
QTM99132.1	0.0	43	1356	32	1247
AIQ34883.1	0.0	43	1355	35	1282
QAS61846.1	0.0	41	1482	40	1410

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6S6Z_A	2.97e-195	56	1336	15	967	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	3.06e-195	56	1336	16	968	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
1F4A_A	1.19e-122	31	634	1	573	E.COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_B E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_C E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_D E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4H_A E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_B E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_C E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_D E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli]
5A1A_A	1.22e-122	31	634	2	574	2.2A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_B 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_C 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_D 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12]
1JZ2_A	1.25e-122	31	634	3	575	E.COLI (lacZ) BETA-GALACTOSIDASE-TRAPPED 2-F-GALACTOSYL-ENZYME INTERMEDIATE (ORTHORHOMBIC) [Escherichia coli],1JZ2_B E. COLI (lacZ) BETA-GALACTOSIDASE-TRAPPED 2-F-GALACTOSYL-ENZYME INTERMEDIATE (ORTHORHOMBIC) [Escherichia coli],1JZ2_C E. COLI (lacZ) BETA-GALACTOSIDASE-TRAPPED 2-F-GALACTOSYL-ENZYME INTERMEDIATE (ORTHORHOMBIC) [Escherichia coli],1JZ2_D E. COLI (lacZ) BETA-GALACTOSIDASE-TRAPPED 2-F-GALACTOSYL-ENZYME INTERMEDIATE (ORTHORHOMBIC) [Escherichia coli],4V40_A BETA-GALACTOSIDASE [Escherichia coli],4V40_B BETA-GALACTOSIDASE [Escherichia coli],4V40_C BETA-GALACTOSIDASE [Escherichia coli],4V40_D BETA-GALACTOSIDASE [Escherichia coli],4V40_E BETA-GALACTOSIDASE [Escherichia coli],4V40_F BETA-GALACTOSIDASE [Escherichia coli],4V40_G BETA-GALACTOSIDASE [Escherichia coli],4V40_H BETA-GALACTOSIDASE [Escherichia coli],4V40_I BETA-GALACTOSIDASE [Escherichia coli],4V40_J BETA-GALACTOSIDASE [Escherichia coli],4V40_K BETA-GALACTOSIDASE [Escherichia coli],4V40_L BETA-GALACTOSIDASE [Escherichia coli],4V40_M BETA-GALACTOSIDASE [Escherichia coli],4V40_N BETA-GALACTOSIDASE [Escherichia coli],4V40_O BETA-GALACTOSIDASE [Escherichia coli],4V40_P BETA-GALACTOSIDASE [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O52847	1.14e-230	43	1352	19	1034	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
Q56307	1.67e-194	56	1336	16	968	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
Q9K9C6	7.53e-178	44	1352	9	1014	Beta-galactosidase OS=Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=lacZ PE=3 SV=1
P23989	3.05e-155	53	1352	19	1025	Beta-galactosidase OS=Streptococcus thermophilus OX=1308 GN=lacZ PE=3 SV=1
Q6LL68	1.70e-153	36	1351	4	1027	Beta-galactosidase OS=Photobacterium profundum (strain SS9) OX=298386 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000295	0.998862	0.000229	0.000228	0.000199	0.000182

TMHMM  Annotations     

start	end
1639	1658