CAZyme Information: MGYG000003724_00006

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Tissierellales; Peptoniphilaceae; Anaerococcus
• CAZyme ID: MGYG000003724_00006
• CAZy Family: GT35
• CAZyme Description: Glycogen phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
748	MGYG000003724_1|CGC1	87827.3	5.549

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003724	1472545	MAG	Canada	North America

• Gene Location: Start: 4832; End: 7078 Strand: +

Full Sequence      

MKINKNTIIERIQNYLYSFYAKDLNTAREREIYDCLCRYIMESVGKTWVSSKKIKEGYEV
YILSFEYLPGSFLSNIIYKLDIEDEIKAAIGEIGFDYDSIMAYDLDPALGIGDMGMGSSY
LINALTDQKIKATAYALRYESGNFKQVIANGKQEVRSDSWLEHGSNWEHQKSFTNLIEIY
GKNHKTVTYDMPILSSDAKFVNTLRLFKSEPTSVIDVKEFSNGDIINAYEDYINNLSITQ
FLYVDDSSYEGKILRLKQEYFFAASAMRDFVRRYLLYYKDINNINKQTNVIINDIHPTLA
LVEFIRILTRVHKFTMKKAIAYTREIFYHLVFSITDDSLEKYPADEIRKLNPELYNTIID
IQAELSKTESFLPLIENGQVIFKHINIALSREYIFLSKVLFENKSLSNNSSYMNMGSDRQ
MYGQSANPKLYDLLREYNINDYSNDSKKLIYLKEDKDFIDSLDEIKYNNKLKLIEYLNDS
SINPYSIFDLQLSIIHESKRQILNAVEIAYEYFYLRDNANAYYVPKTYIFSGKANEGYYV
AKETIKFILALKKLIEADKIISKKIKIIFVENTGVKDLRILYPAADIYSSLAHPLMDNQN
FDILNCLYNMSNVVSTKAGIVENLDIANSFYLMDDDYKSYEIIRNERKYIANDIIYQNKI
VKYTIDRLLNEPKEAFPYDFKNLYDEILLYNDSFNVFLDLENLVNLRKDIQKDYVDKEKW
IQNQMQNFIWASKFNLDNLLKRKRSFDK

Enzyme Prediction     

No EC number prediction in MGYG000003724_00006.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	89	741	8.5e-116	0.983679525222552

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04300	GT35_Glycogen_Phosphorylase	2.04e-107	9	737	1	782	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
pfam00343	Phosphorylase	9.09e-86	92	722	3	633	Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin.
PRK14986	PRK14986	3.95e-79	59	741	71	801	glycogen phosphorylase; Provisional
COG0058	GlgP	8.81e-74	2	737	3	735	Glucan phosphorylase [Carbohydrate transport and metabolism].
PRK14985	PRK14985	1.11e-49	60	587	61	630	maltodextrin phosphorylase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACV28899.1	5.28e-281	2	742	7	747
QQB62731.1	1.21e-239	2	737	3	742
QQN55753.1	1.95e-238	2	737	3	742
CED93052.1	8.69e-103	2	740	3	794
QZO77069.1	1.27e-101	11	687	10	690

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3CEH_A	5.33e-78	15	741	12	796	Humanliver glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE5688 [Homo sapiens],3CEH_B Human liver glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE5688 [Homo sapiens],3CEJ_A Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE2865 [Homo sapiens],3CEJ_B Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE2865 [Homo sapiens],3CEM_A Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE9423 [Homo sapiens],3CEM_B Human glycogen phosphorylase (tense state) in complex with the allosteric inhibitor AVE9423 [Homo sapiens]
1XOI_A	9.84e-78	15	741	34	818	HumanLiver Glycogen Phosphorylase A complexed with Chloroindoloyl glycine amide [Homo sapiens],1XOI_B Human Liver Glycogen Phosphorylase A complexed with Chloroindoloyl glycine amide [Homo sapiens]
1FA9_A	9.84e-78	15	741	34	818	HUMANLIVER GLYCOGEN PHOSPHORYLASE A COMPLEXED WITH AMP [Homo sapiens]
1FC0_A	9.84e-78	15	741	34	818	HUMANLIVER GLYCOGEN PHOSPHORYLASE COMPLEXED WITH N-ACETYL-BETA-D-GLUCOPYRANOSYLAMINE [Homo sapiens],1FC0_B HUMAN LIVER GLYCOGEN PHOSPHORYLASE COMPLEXED WITH N-ACETYL-BETA-D-GLUCOPYRANOSYLAMINE [Homo sapiens],2ATI_A Glycogen Phosphorylase Inhibitors [Homo sapiens],2ATI_B Glycogen Phosphorylase Inhibitors [Homo sapiens]
2QLL_A	1.00e-77	15	741	35	819	Humanliver glycogen phosphorylase- GL complex [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0VCM4	8.99e-80	15	741	35	819	Glycogen phosphorylase, liver form OS=Bos taurus OX=9913 GN=PYGL PE=2 SV=1
P45180	1.98e-79	27	741	41	805	Glycogen phosphorylase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=glgP PE=3 SV=1
Q9CN90	1.32e-78	27	740	41	804	Glycogen phosphorylase OS=Pasteurella multocida (strain Pm70) OX=272843 GN=glgP PE=3 SV=1
P0AC87	2.41e-78	21	741	30	801	Glycogen phosphorylase OS=Shigella flexneri OX=623 GN=glgP PE=3 SV=1
P0AC86	2.41e-78	21	741	30	801	Glycogen phosphorylase OS=Escherichia coli (strain K12) OX=83333 GN=glgP PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000054	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003724_00006.