CAZyme Information: MGYG000003742_00284

Basic Information

• Species: Prevotella buccalis
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella buccalis
• CAZyme ID: MGYG000003742_00284
• CAZy Family: GH76
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1325	MGYG000003742_5|CGC1	147399.73	5.5828

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003742	2540681	MAG	Canada	North America

• Gene Location: Start: 55754; End: 59731 Strand: +

Full Sequence      

MIPTKKKILLLSVFCFLMQLAWGAVVEGKTYRLVSVGAKDKSLFIENSSLQSNANILLWT
DMNVPSQQWEAVRNNDGSFAFKNVYSGKFLTRRGMLGKTSQLYQTTFSTFSSSKWKVEAV
AGQADQYQLVQQDGGQSYCITTGSTNDGAALAVDVVQKNQTGGADPSQIWQLVEVEPINQ
VTPALRDEVMNGWTKQFVRARGANVSFGIGGGWGDAEMLETMLDAYETSGQEAYLDLFKK
GFNYFKSKVKDNWLRHYWFGYRWNGQDYNDDVMWMIIASARAGLLTGSTTYTQLAKDNFD
AIYQRAYNPWGMLRWAESSGDRNGTNSCVNGPAEVAACYIAMATGDETYYAKARDLYAKQ
RRYLFNASTGQVYDSFIWDATTNLPSKYNRWASTYNQGTMLGAAVMLYNHYGDEQYKQDA
ERIMEYTVKHLCNQDGIIQVCQVVDGDLSGFKGILMRYVRKFIIDLNKPEYVEWMQKNAM
QAYSNMNSRGVIHSAWLTKSSEDFMFGEKKFDNQPFGCSTAVSVAFNAPLNDSRIVKDAF
QPIRADEFNAIQGVYLKDSNSGKSPFEISNIKNDYYVGYNWVDFGSQPATSVSFYVSELG
NASYQCAIEVHLDSIDGPCIGTAAIPNTPGWKTVTAPVAPTDGMHQVYLKFVNKENVAAV
DNFRLAEMQFGTNREALPGDITDNGGQLMTDQQVDDLGCLTDNRLTTSVTVSAFKGQAMF
HYRSVAPALLKGYAIGSGTGDASFDAKSWTLQGSNDGQTWTTLDTQEAQAFSTRCQNKHF
SVNTSKTFTDYRLLVTATNGGKALHIGEWQLLGTCLLSDDITADGGSMMHQEGSSLIDKN
ASTMASFDANQDVVIEYKAAGSYVLTSYSLTNGDSKAAENPAGWVLYGSSDGRNWTELDR
KRHETFPVHQSTQFYTVASQEAYKSFRLVITGNHGATHTALHEWQLIGKLISSAPLYNDI
TKNGGKLTASNGANHADLQVLTDNNARTYAELPFKDEAWVQYQTPEPAKVTAYSICMGYD
ADKDPRTWQLQASNDGTNWRVLHQGSASNVKEKGTLRTFKITNNDLYVYYRLLVKKTASA
SATSVMLGELQLHGLCVSNQDLTSQEGTTSCYVPGVNGGEAVDKLFDKAASSKYCFSFYG
ESWVDYQAAEGMKANLYSLTSANDNADRDPQSWKVLGSHDGNHWVVLDERNDELFYDRHI
TQFYSFDNSEPYEYYRLQLDENKGSELCQISEFQLFYSDVVATGVKAIQTPHLAVKVYPN
PVIDYLHVDLPAEGRIQVFDSQGLLMAQQRAKKGNNQVDFTGVAPGLYLVKVVCGTQTKT
FKVVK

Enzyme Prediction     

No EC number prediction in MGYG000003742_00284.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH76	192	499	3e-54	0.835195530726257
CBM6	547	665	1.5e-18	0.8333333333333334

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam03663	Glyco_hydro_76	2.28e-41	213	490	36	320	Glycosyl hydrolase family 76. Family of alpha-1,6-mannanases.
cd04084	CBM6_xylanase-like	1.77e-20	541	670	1	123	Carbohydrate Binding Module 6 (CBM6); many are appended to glycoside hydrolase (GH) family 11 and GH43 xylanase domains. This family includes carbohydrate binding module 6 (CBM6) domains that are appended mainly to glycoside hydrolase (GH) family domains, including GH3, GH11, and GH43 domains. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB6s belonging to this family are Microbispora bispora GghA, a 1,4-beta-D-glucan glucohydrolase (GH3); Clostridium thermocellum xylanase U (GH11), and Penicillium purpurogenum ABF3, a bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase (GH43). GH3 comprises enzymes with activities including beta-glucosidase (hydrolyzes beta-galactosidase) and beta-xylosidase (hydrolyzes 1,4-beta-D-xylosidase). GH11 family comprises enzymes with xylanase (endo-1,4-beta-xylanase) activity which catalyze the hydrolysis of beta-1,4 bonds of xylan, the major component of hemicelluloses, to generate xylooligosaccharides and xylose. GH43 includes beta-xylosidases and beta-xylanases, using aryl-glycosides as substrates. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold.
smart00606	CBD_IV	7.58e-19	537	665	2	129	Cellulose Binding Domain Type IV.
pfam03422	CBM_6	1.50e-17	545	665	2	123	Carbohydrate binding module (family 6).
COG4833	COG4833	7.44e-12	213	466	46	281	Predicted alpha-1,6-mannanase, GH76 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT74831.1	9.15e-278	6	1325	2	1310
QIA07414.1	7.32e-96	27	672	66	678
QEC70572.1	3.59e-74	27	527	30	494
AYD48939.1	6.64e-71	25	528	28	495
QOV19890.1	4.03e-67	210	663	56	489

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5M77_A	1.17e-42	213	651	73	506	ChainA, Alpha-1,6-mannanase [Niallia circulans],5M77_B Chain B, Alpha-1,6-mannanase [Niallia circulans]
4BOK_A	4.10e-32	213	496	39	317	ChainA, Alpha-1,6-mannanase [Niallia circulans]
4BOJ_A	4.37e-32	213	496	42	320	ChainA, Alpha-1,6-mannanase [Niallia circulans],4BOJ_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4BOJ_C Chain C, Alpha-1,6-mannanase [Niallia circulans]
4D4A_A	4.68e-32	213	496	60	338	ChainA, Alpha-1,6-mannanase [Niallia circulans],4D4A_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4D4B_A Chain A, Alpha-1,6-mannanase [Niallia circulans],4D4B_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4D4C_A Chain A, Alpha-1,6-mannanase [Niallia circulans],4D4C_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4D4D_A Chain A, Alpha-1,6-mannanase [Niallia circulans],4D4D_B Chain B, Alpha-1,6-mannanase [Niallia circulans],5N0F_A Chain A, Alpha-1,6-mannanase [Niallia circulans],5N0F_B Chain B, Alpha-1,6-mannanase [Niallia circulans],6ZBX_A Chain A, Alpha-1,6-mannanase [Niallia circulans],6ZBX_B Chain B, Alpha-1,6-mannanase [Niallia circulans],7NL5_A Chain A, Alpha-1,6-mannanase [Niallia circulans]
5AGD_A	2.11e-31	213	496	60	338	ChainA, Alpha-1,6-mannanase [Niallia circulans],5AGD_B Chain B, Alpha-1,6-mannanase [Niallia circulans],6ZBM_A Chain A, Alpha-1,6-mannanase [Niallia circulans],6ZBW_A Chain A, Alpha-1,6-mannanase [Niallia circulans],6ZBW_B Chain B, Alpha-1,6-mannanase [Niallia circulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P33558	1.98e-06	531	663	241	368	Endo-1,4-beta-xylanase A OS=Thermoclostridium stercorarium OX=1510 GN=xynA PE=1 SV=2
Q8GJ44	2.30e-06	531	663	240	367	Endo-1,4-beta-xylanase A OS=Thermoclostridium stercorarium OX=1510 GN=xynA PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000303	0.999111	0.000168	0.000140	0.000130	0.000127

TMHMM  Annotations     

start	end
7	26