CAZyme Information: MGYG000003754_00161

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Porphyromonadaceae; Porphyromonas
• CAZyme ID: MGYG000003754_00161
• CAZy Family: GT19
• CAZyme Description: Lipid-A-disaccharide synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
376		42189.03	9.7392

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003754	1700916	MAG	Canada	North America

• Gene Location: Start: 23803; End: 24933 Strand: -

Full Sequence      

MRYFLVAGEASGDLHAGELISALRKLDPEAEFAFMGGEAMAKAAGTSPILHYKKIAYMGF
GPVIRNYRSIRHAAKEIQRTMLRFAPDRVIAIDSSGFNLRYILSFAYKALPEAKRFYYIA
PKVWAWKTDRVKLLRKYCHEVLSILPFEEAFFLNHNIPCTYVGNPTVEELAPYCSTDGHI
GSNNQQQLPISEKRPLIALLPGSRKQEIRRNLKLMTEVAASFTDYHFVIAAAPGIEDSFY
TPYLTDHVSIVRNSTYALLATSKAALVTSGTATLETALIGTPQVVIYRINGQRILNILFD
KLIKVPYISLVNLIAGREVVTELLAADATVQRIHKELTKILQPAHVEEIRKGYEQVRVTL
GKKNAAMGAAQRIYYY

Enzyme Prediction     

No EC number prediction in MGYG000003754_00161.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT19	4	359	7.4e-104	0.9858757062146892

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0763	LpxB	2.10e-99	1	373	2	375	Lipid A disaccharide synthetase [Cell wall/membrane/envelope biogenesis].
PRK00025	lpxB	4.65e-93	1	373	2	371	lipid-A-disaccharide synthase; Reviewed
pfam02684	LpxB	4.48e-81	4	371	2	369	Lipid-A-disaccharide synthetase. This is a family of lipid-A-disaccharide synthetases, EC:2.4.2.128. These enzymes catalyze the reaction: UDP-2,3-bis(3-hydroxytetradecanoyl) glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate <=> UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6 -beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. These enzymes catalyze the fist disaccharide step in the synthesis of lipid-A-disaccharide.
TIGR00215	lpxB	1.30e-64	5	371	10	380	lipid-A-disaccharide synthase. Lipid-A precursor biosynthesis producing lipid A disaccharide in a condensation reaction. transcribed as part of an operon including lpxA [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]
PRK14089	PRK14089	4.10e-48	1	331	1	307	lipid-A-disaccharide synthase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALO28862.1	1.03e-105	1	373	1	369
QUI91423.1	1.46e-105	1	373	1	369
ATS02318.1	1.46e-105	1	373	1	369
AIJ35071.1	1.46e-105	1	373	1	369
AUR47837.1	1.46e-105	1	373	1	369

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5W8N_A	4.62e-34	5	373	11	377	LipidA Disaccharide Synthase (LpxB)-6 solubilizing mutations [Escherichia coli BL21(DE3)]
5W8S_A	4.62e-34	5	373	11	377	LipidA Disaccharide Synthase (LpxB)-7 solubilizing mutations [Escherichia coli BL21(DE3)],5W8X_A Lipid A Disaccharide Synthase (LpxB)-7 solubilizing mutations-Bound to UDP [Escherichia coli BL21(DE3)]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q1D393	2.07e-56	2	373	6	374	Lipid-A-disaccharide synthase OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=lpxB PE=3 SV=1
B6J161	2.44e-56	5	374	8	371	Lipid-A-disaccharide synthase OS=Coxiella burnetii (strain CbuG_Q212) OX=434923 GN=lpxB PE=3 SV=1
A9KC41	3.43e-56	5	374	8	371	Lipid-A-disaccharide synthase OS=Coxiella burnetii (strain Dugway 5J108-111) OX=434922 GN=lpxB PE=3 SV=1
Q83DS5	3.43e-56	5	374	8	371	Lipid-A-disaccharide synthase OS=Coxiella burnetii (strain RSA 493 / Nine Mile phase I) OX=227377 GN=lpxB PE=3 SV=1
A9NCA7	3.43e-56	5	374	8	371	Lipid-A-disaccharide synthase OS=Coxiella burnetii (strain RSA 331 / Henzerling II) OX=360115 GN=lpxB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000075	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003754_00161.