CAZyme Information: MGYG000003755_00517

Basic Information

• Species: Arcanobacterium_A urinimassiliense
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Arcanobacterium_A; Arcanobacterium_A urinimassiliense
• CAZyme ID: MGYG000003755_00517
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
653	MGYG000003755_96|CGC1	72819.55	7.7432

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003755	1072433	MAG	Canada	North America

• Gene Location: Start: 1001; End: 2962 Strand: -

Full Sequence      

MPDISSTAKPQIPLPEYPRPQLQRSAATWKSLNGYWRYGIEAGDLAALRQRYGEKATANE
REKFCPPPLTGKILVPFSPEAPLSGVGKRLLPGELLYYERTLSPDELPDSFIFPNSDSSS
STCPPARLLLHFGAIDQNCLVFINGHYAGENRGGYFAFTLDITSYLHPGEQKIQVVVEDP
SNQGQGAYGKQSLQPGNIWYRPSSGIWQSVWLEAVPTSYISAVQITPYFDSGAVELEVQI
ESPLSTTTTTGITPASSLNAAASTAANSLPIQGKILAPAAAGLQGESIELGSFEIKAVST
STATLYGKLPPDFRPWSPEDPYLYRYELQYGEDLLQGYFGMRKFSLISTKNGVQRLALNN
RPYVHMGVLDQGYWSEGFYTPPSDADLIADIKSMQALGFNMLRKHIKIESARAYYHCDRL
GMLIWQDMVSGGGPYCKPAISVLPFLGIHFNDVRWAHLFGRRNSESKDLYNQELLRTVKQ
LYSVPSIALWVPFNEGWGQFSALAAAKKIRDLDPTRLVDHASGWHDQGGGDVKSRHIYFR
KPRMRLDKKGRALALSEFGGYSFAVPGHCYSSHSFGYRRYKDIANYQQAVLELFRFLLNT
GKDISAYVYTQLSDVEDEVNGFLTYDRAVNKWPDSSLAAAELRSLLLTLRKQI

Enzyme Prediction     

No EC number prediction in MGYG000003755_00517.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	23	535	1.6e-82	0.4933510638297872

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.07e-21	30	518	13	429	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.71e-13	127	518	83	445	beta-D-glucuronidase; Provisional
PRK10340	ebgA	5.53e-10	127	518	127	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	5.04e-08	127	216	83	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
PRK09525	lacZ	4.75e-07	161	422	173	408	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACZ86262.1	5.98e-178	10	633	13	570
SCG46038.1	1.55e-172	7	638	12	575
QSH92753.1	4.28e-172	13	642	23	602
AWB88178.1	1.24e-171	14	643	17	582
QKW37630.1	1.08e-170	6	643	9	576

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	1.04e-97	14	631	26	569	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
3FN9_A	5.26e-10	127	559	78	486	Crystalstructure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_B Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_C Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_D Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343]
6D1N_A	2.31e-08	29	521	43	451	Apostructure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D1N_B Apo structure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D41_A Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D41_B Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D6W_A Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_B Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_C Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_D Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D7F_A Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_B Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_C Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_D Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_E Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_F Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis]
6D89_A	5.25e-08	94	521	93	443	Bacteroidesuniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_B Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_C Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_D Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis]
7VQM_A	6.99e-08	32	569	16	480	ChainA, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_B Chain B, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_C Chain C, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_D Chain D, GH2 beta-galacturonate AqGalA [Aquimarina sp.]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	1.17e-13	130	495	124	430	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P00723	1.07e-06	130	561	127	543	Beta-galactosidase OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=LAC4 PE=1 SV=2
P77989	6.60e-06	127	428	74	347	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000075	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003755_00517.