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CAZyme Information: MGYG000003760_00084

You are here: Home > Sequence: MGYG000003760_00084

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species
Lineage Bacteria; Firmicutes_A; Clostridia; Tissierellales; Peptoniphilaceae; Anaerococcus;
CAZyme ID MGYG000003760_00084
CAZy Family GH101
CAZyme Description Endo-alpha-N-acetylgalactosaminidase
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
1951 MGYG000003760_1|CGC2 221637.84 4.4799
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000003760 1804087 MAG Canada North America
Gene Location Start: 88931;  End: 94786  Strand: -

Full Sequence      Download help

Enzyme Prediction      help

EC 3.2.1.97

CAZyme Signature Domains help

Family Start End Evalue family coverage
GH101 377 1099 0 0.9957567185289957

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
pfam12905 Glyco_hydro_101 1.12e-141 643 934 1 273
Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244 GH_101_like 6.82e-120 656 957 1 298
Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam17974 GalBD_like 1.22e-92 1269 1459 1 190
Galactose-binding domain-like. Proteins containing a galactose-binding domain-like fold can be found in several different protein families, in both eukaryotes and prokaryotes. The common function of these domains is to bind to specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase, phospholipids on the outer side of the mammalian cell membrane for coagulation factor Va, membrane-anchored ephrin for the Eph family of receptor tyrosine kinases, and a complex of broken single-stranded DNA and DNA polymerase beta for XRCC1. The structure of the galactose-binding domain-like members consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold.
pfam18080 Gal_mutarotas_3 5.27e-82 374 642 1 243
Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17995 GH101_N 1.00e-63 169 348 1 180
Endo-alpha-N-acetylgalactosaminidase N-terminal. This is the N-terminal domain found in Streptococcus pneumoniae endo-alpha-N-acetylgalactosaminidase (EC:3.2.1.97), a cell surface-anchored glycoside hydrolase from family GH101 involved in the breakdown of mucin type O-linked glycans. This is a twisted beta-sandwich domain composed of two sheets of six and seven antiparallel beta-strands. The domain appears to be missing the extended metal and carbohydrate-binding loops.

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
ACV29694.1 0.0 1 1951 1 1968
QQB61808.1 0.0 162 1949 119 1814
AYQ24117.1 0.0 186 1774 235 1804
QQA36326.1 0.0 168 1737 157 1697
QQQ35090.1 0.0 186 1749 419 1966

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
3ECQ_A 0.0 184 1623 106 1530
Endo-alpha-N-acetylgalactosaminidasefrom Streptococcus pneumoniae: SeMet structure [Streptococcus pneumoniae R6],3ECQ_B Endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae: SeMet structure [Streptococcus pneumoniae R6]
6QEP_A 0.0 367 1582 5 1230
EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
5A59_A 0.0 361 1480 6 1110
Thestructure of GH101 E796Q mutant from Streptococcus pneumoniae TIGR4 in complex with T-antigen [Streptococcus pneumoniae TIGR4],5A5A_A The structure of GH101 E796Q mutant from Streptococcus pneumoniae TIGR4 in complex with PNP-T-antigen [Streptococcus pneumoniae TIGR4]
5A58_A 0.0 361 1480 6 1110
Thestructure of GH101 D764N mutant from Streptococcus pneumoniae TIGR4 in complex with serinyl T-antigen [Streptococcus pneumoniae TIGR4]
5A55_A 0.0 361 1480 8 1112
Thenative structure of GH101 from Streptococcus pneumoniae TIGR4 [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
Q2MGH6 0.0 184 1735 142 1677
Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60 0.0 184 1735 142 1677
Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
A9WNA0 3.73e-109 369 1478 47 1042
Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2

SignalP and Lipop Annotations help

This protein is predicted as SP

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
0.273761 0.713867 0.009673 0.001320 0.000635 0.000739

TMHMM  Annotations      download full data without filtering help

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