CAZyme Information: MGYG000003764_00139

Basic Information

• Lineage: Bacteria; Campylobacterota; Campylobacteria; Campylobacterales; Campylobacteraceae; Campylobacter_B
• CAZyme ID: MGYG000003764_00139
• CAZy Family: GT19
• CAZyme Description: Lipid-A-disaccharide synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
357		40152.46	9.6788

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003764	1086320	MAG	Canada	North America

• Gene Location: Start: 3497; End: 4570 Strand: +

Full Sequence      

MAKKLLISALEPSANLHLGEILKELKDYEICGIFDEKFGSPYAKSSEFSAMGFFEILPLI
LKAKRALKALVKMARECDAVLLIDSPAFNIPLAKALKQAGISAKITYFILPQVWAWKPGR
AKIVEENCDNLAAIWDFERKFYPKCHYVGSPLLDEINRAQILARNLEQNKQNLPEKIAFL
PGSRIGEIKRLMPIFRDLATKFKDKKLILPIPANLMARKDEIYGDLRAFEISNDAVLAMS
GAKFAFICSGTATMQAALIGTPFVLCYKAKPIDIFIARKFVKLKYAGLANIILDFLGEKP
LNLELIQEQVTCENLFKAYENEIKNVAKFDSVSKRLREYLSHGAAKNVANLIKFNQL

Enzyme Prediction     

EC	2.4.1.182

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT19	6	338	7.3e-88	0.9745762711864406

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14089	PRK14089	1.49e-175	3	354	2	346	lipid-A-disaccharide synthase.
COG0763	LpxB	9.09e-94	2	345	1	367	Lipid A disaccharide synthetase [Cell wall/membrane/envelope biogenesis].
pfam02684	LpxB	7.41e-78	5	354	1	361	Lipid-A-disaccharide synthetase. This is a family of lipid-A-disaccharide synthetases, EC:2.4.2.128. These enzymes catalyze the reaction: UDP-2,3-bis(3-hydroxytetradecanoyl) glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate <=> UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6 -beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. These enzymes catalyze the fist disaccharide step in the synthesis of lipid-A-disaccharide.
PRK00025	lpxB	6.80e-75	2	353	1	376	lipid-A-disaccharide synthase; Reviewed
TIGR00215	lpxB	7.96e-73	5	354	8	385	lipid-A-disaccharide synthase. Lipid-A precursor biosynthesis producing lipid A disaccharide in a condensation reaction. transcribed as part of an operon including lpxA [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCD53290.1	1.05e-143	1	353	1	341
AQW88320.1	5.64e-142	1	352	1	341
AQW86670.1	1.14e-141	1	352	1	341
AQW83549.1	2.64e-140	1	352	1	341
QKF70616.1	5.29e-140	3	355	2	351

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5W8N_A	4.01e-22	78	318	92	340	LipidA Disaccharide Synthase (LpxB)-6 solubilizing mutations [Escherichia coli BL21(DE3)]
5W8S_A	4.01e-22	78	318	92	340	LipidA Disaccharide Synthase (LpxB)-7 solubilizing mutations [Escherichia coli BL21(DE3)],5W8X_A Lipid A Disaccharide Synthase (LpxB)-7 solubilizing mutations-Bound to UDP [Escherichia coli BL21(DE3)]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A8FK76	1.09e-109	3	352	2	361	Lipid-A-disaccharide synthase OS=Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828) OX=407148 GN=lpxB PE=3 SV=1
Q5HWH9	1.09e-109	3	352	2	361	Lipid-A-disaccharide synthase OS=Campylobacter jejuni (strain RM1221) OX=195099 GN=lpxB PE=3 SV=1
Q9PIK8	1.77e-108	3	352	2	361	Lipid-A-disaccharide synthase OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) OX=192222 GN=lpxB PE=3 SV=1
B2USW3	1.34e-82	5	354	4	355	Lipid-A-disaccharide synthase OS=Helicobacter pylori (strain Shi470) OX=512562 GN=lpxB PE=3 SV=1
B5Z7M7	1.89e-82	5	354	4	355	Lipid-A-disaccharide synthase OS=Helicobacter pylori (strain G27) OX=563041 GN=lpxB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000059	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003764_00139.