CAZyme Information: MGYG000003768_00205

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Clostridium_A
• CAZyme ID: MGYG000003768_00205
• CAZy Family: CBM32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1504		165981.15	4.0533

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003768	1681255	MAG	Canada	North America

• Gene Location: Start: 46472; End: 50986 Strand: -

Full Sequence      

MKKFVKRLLGLTLAACMVMSATTVSAARQEWSATEPVSISAMDSGDTNLALGKSVTANEV
EPGTRFTADLAVDGDTTTRWASDAMKNTPGDKWLRVDFGEEITFDTVNILWEQLNILHYR
LEVSNDISSEDNWTTIYESDSRISTKDESILLPQDATGRYLRVYVTDCDGAESNWDSVSI
FELGVYYSGGGSEPEEPTGNYVVYPVPQKVTDSEETVQLTDTIHVIKEEGIDTVTQNRIE
EVLTEHGYAVEYSNIAVEGETNLYIGINGSGGAADTYDGIPKDVFTEGENKYDMHVVEIN
KNGDIVILGKDNDAAFYGLATLEQVLDQAEDHTLKVSLFEDYSNQKYRGAVEGYYGYPWS
VDGTLSWMDYAKRYKMNIFLYGPKSDPYHLGKWKEEYPVELTEEEKAHGVLSQDDIRQIT
AKAAECNVDFVWVAHPAMQNGINFGSNQTIDQGVQDLMVKFDHMYDLGVREFGVFLDDIP
YGSATGTGHAYLIDQVQKKLYETYNTEGTAPEDQIKPLFFTPTYYTVYGASSSYLSAFRN
IHEDVVICFTGNDVFSDISNEDAAQYEEWIGREPCLWWNYPVNDNDDNVFYTCPIDSFYS
QDSDISNFAGIISNPMNFEEASKVAFFAIADYSWNPGAFNAQQNWNHCFDSIIPDDPEMA
EALQVVYGCLNKRVEPMKLRKLYNQYKSEAGTGSTEAAVALRDKMAEIIDSIGKIETLKD
SENPAYRLLVSEAQTSMNKLYDMAVTIKGVLNATLETDPLTIYQGYYSARLAYDRLNIVE
NPRYEMTSLEGAGEEITVNKLSAVPSDTYMRPFVDYIIGELDTVELPDLDPSLAGVKSVT
ITSGDDQVKQGETGQYSASVVAEREDAVEVVWSVEGNTDKTTTISSNGLLTMGKNELAQE
ITVRATSAYDPSKSDTTQVTVVDRVYEDPTIPTNVAFNAVVLGSTGDPTGVEVPERALDE
NEETKWCPGSNTKTNQWMAIDLGATKTISEWKVVHAGSEGAAYITRDFSLQVLKDPNATE
EQLKDPDYLGNDSNWQTIKHITNNSNNLTDITFEEPIEGRYFRLFVADGGSGGYPATRIY
EWQMMGVDTEIVSQTYNLSVDPAIEHGSIHIESAHYQAGARVNLQIVPEEGYQLKAGSLQ
YNGNEITDNRFIMPEEDVVITAEFELISSTETYQVTVSDTENGEVTVNPAEAEAGTVITV
TVSPEEGYRLVEGSLKANGVVIENNQFIMPAEDVTVTAEFEKIPVQVVTKVLEKILEKAE
FLWQSGALENTMEAVVTEFQNAVEAGKQLLADPSQATQEQINDATERLLKVMGKVDWKQG
DKTVLQVAVEIARVIYEDIDRYVEEGKQEFLDALARGEELLSSGNAWDDEVQEAADRLIE
AMSNLRMAPNKDILNDMIKQAGSIDLSLYTADSTAALKGAVAQARAVAADVNADQGDIDA
AVESLQAALKGLILNQPSGENSETVTVGEGAIPTKTGDSGSSMILVGLIAAAGAVLVLSR
KNRR

Enzyme Prediction     

No EC number prediction in MGYG000003768_00205.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	348	665	1.5e-73	0.9864406779661017
CBM32	63	183	2.1e-22	0.8709677419354839
CBM32	949	1081	2.7e-16	0.8629032258064516

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	3.19e-106	348	665	1	292	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam00754	F5_F8_type_C	4.83e-16	54	183	1	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam18998	Flg_new_2	1.41e-13	1173	1244	1	74	Divergent InlB B-repeat domain. This family of domains are found in bacterial cell surface proteins. They are often found in tandem array. This domain is closely related to pfam09479.
pfam00754	F5_F8_type_C	2.71e-13	953	1078	13	124	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam02838	Glyco_hydro_20b	4.25e-11	200	341	1	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCI64753.1	1.81e-146	200	872	22	707
QUT77745.1	2.58e-145	201	821	21	647
AHF13697.1	1.68e-143	201	842	25	682
AHF13792.1	2.02e-141	200	1100	8	916
QFQ11845.1	5.44e-103	200	817	23	651

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PWI_A	2.39e-81	177	773	11	581	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6PV4_A	6.14e-68	201	775	32	611	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
6PV5_A	7.34e-56	198	665	37	485	Structureof CpGH84B [Clostridium perfringens ATCC 13124]
5MI4_A	1.17e-49	304	662	100	431	BtGH84mutant with covalent modification by MA3 [Bacteroides thetaiotaomicron VPI-5482],5MI5_A BtGH84 mutant with covalent modification by MA3 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482],5MI6_A BtGH84 mutant with covalent modification by MA3 in complex with Thiamet G [Bacteroides thetaiotaomicron VPI-5482],5MI7_A BtGH84 mutant with covalent modification by MA4 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482]
2J4G_A	1.35e-49	304	662	88	419	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482],2J4G_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	4.27e-64	201	775	39	618	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q89ZI2	9.71e-49	304	662	110	441	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
Q0TR53	4.04e-43	205	847	48	637	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
Q8XL08	5.33e-43	205	847	48	637	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q8VIJ5	7.57e-15	349	665	63	357	Protein O-GlcNAcase OS=Rattus norvegicus OX=10116 GN=Oga PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000816	0.783541	0.214664	0.000383	0.000307	0.000259

TMHMM  Annotations     

start	end
1480	1499