CAZyme Information: MGYG000003769_00896

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium
• CAZyme ID: MGYG000003769_00896
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1160	MGYG000003769_6|CGC1	132245.81	4.7384

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003769	4856301	MAG	Canada	North America

• Gene Location: Start: 60532; End: 64014 Strand: +

Full Sequence      

MVRLFNDEWRFLKTNLDGAKDEVLVSLDQFSPVEVPHDWLIYNVHNLYENSCGWYYKKFQ
LSSTLDSLERVILRFDGVYMDSTVYINNQKVGDWKYGYSAITLDITKELRDGENEVLVQV
RYQSPNSRWYSGAGVYRNVWIKECSRAYLPLDGSYITSKVQGDDYLLEVGTEVDGQITNN
IMCQYSLWYEDEMVQDFGLKKVSIGNSDINATNTASLKELVKNPNIWDVESPHCYELRVT
LYDMSNEEELDSERITIGFRTMKFDPEEGFILNGRRLKVHGVCEHHDFGCLGAAFHKEAM
RRKFRILREMGVNALRTSHNMPAKEVMELADEMGFLVVNEAFDMWERSKTEYDYGRFFLE
WANEDVKSWIRRDRNHPSLMLWSIGNEIYDTHADEYGQEITKRLVAMVREHDPKENALIT
IGSNYMPWENARKCADIVKIAGYNYAEKYYEAHHEKYKDWVIYGSETASLVQSRGVYKFP
LSRTILSDEDEQCSALGNSTTSWGAKSVEQCIIDDRDAEFCFGQFIWTGFDYIGEPTPYH
TKNSYFGQIDTAGFPKDAFYIFKSAWTDATKNPMVHVFPYWDFNEGQLIDIRACTNGAMV
ELFVNDISYGRQAIDHINGKKLLGEWKVPFSRGTIKAIAYDIDGKPIACEVRHSFFDAKE
IVLSTNQTTLLANGEDLCFLTISVVDENQNPVENAMNYVKVDIDGSGRLLGLDNGDSTDY
DQYKSNVRKLFQGKLLAVIGSTFEEGEINVTVSGEGLKSSSIKLIAMNSEIRKGICARED
FRDCDRSSVGHPNIPVRKIELSYEEGNELSENVKELICNAYIYPKNATDTDLTWMLVNNA
GIEISYAVIEEIRNEGNVHSARIKAYGDGEFYVKCMSKSGTDKIKIISAIEAYAKGLGQA
YLNPYEFISAGLFSDTIGDIGNGNERGISTSRDGMSGVIYKNIDFGDYGSDEITIPIFAL
SSERYEIEIWQGSPLEEESEKLTTVSYQKESIWNTYQEETYRLPCRIKGIATIAFVLKNK
VHIKGFCFKKLNKAISKINAAECNRVYGDDFLIEPEAVTGIGNNVSLEFESMDFGEEGVS
RIEICGRTPLPVNTIHIHLTKEDGTKQNCIVEFDKRDGSSSNLDYVSQSFEIDKFKGKGM
VTFVFLPGSKFDFHSFQFMR

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	564	1.1e-103	0.6037234042553191

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.47e-61	1	582	11	614	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	3.47e-38	661	763	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	3.66e-38	54	413	68	441	beta-D-glucuronidase; Provisional
PRK10340	ebgA	5.74e-30	49	387	107	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.32e-28	2	387	52	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QYR22526.1	0.0	4	1160	6	1146
AIQ25741.1	0.0	3	1158	5	1145
SQI53931.1	0.0	4	1158	6	1140
QNF28870.1	0.0	4	1160	6	1146
QZN78011.1	0.0	3	1160	5	1162

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	6.22e-171	5	769	6	804	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	5.94e-160	5	767	12	808	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
4CU6_A	4.77e-148	5	770	18	848	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	1.31e-147	5	770	18	848	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]
5T98_A	3.29e-140	5	760	30	814	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	1.60e-151	5	766	47	848	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	7.64e-89	5	753	51	807	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.87e-48	6	688	57	753	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	6.76e-45	5	748	8	719	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	1.09e-32	5	775	31	804	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000064	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003769_00896.