CAZyme Information: MGYG000003769_00998

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium
• CAZyme ID: MGYG000003769_00998
• CAZy Family: GH94
• CAZyme Description: 1,2-beta-oligoglucan phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1113	MGYG000003769_7|CGC1	126548.68	5.4933

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003769	4856301	MAG	Canada	North America

• Gene Location: Start: 35892; End: 39233 Strand: -

Full Sequence      

MVLKTLSAPITLKNGNSAFTFLSGGDSFEWIHESVLINSFRGNTLNGSANNIYLRIYKDD
TLTFYPLVGLASKSSLKSSNSSLMFEGIVEQISYTVTFRLTSYGIWFWDISLNGKCDKAD
IIYSQDIGIGSKGSINTNELYTAQYLGHSIFEGDNGYVICSRQNMPQNESYPYLQQGSLG
IRSIAYSADGTQFFGLSYKKTDIPEALLGNLPSKNKQYELSHTALQTESFSLSGTKQFSF
YGICKSNHPEAIKEIEYIDELQKVYSSKESQDFQTVKVPSLTKTGAPYASKQWNIEQLNQ
YFPDRLLEEKESDTLLSFFTPTNGHVVLQAKEMITERPHGHILMTDFDLTKVPQGVVSST
NYIYGVFNCQLVVGNTTYNKLLSNHRGLLNVEKHSGQRIYVKIGDSYRLLTLPAAYEMDV
AGSTWYYQLEDDILIVTSFAMYNRPEIVLKVQSINNITYDFIVTNQLTAGPNEYENDIVL
TKEDNILHIHPTSDVFTNSYYPDLHFRMKIPKNSTITDDSFFFEDNTTINPTLLSVVIPN
SSNFEIVMQGYDTKNDIPFLDNYDYPAQKEAYHKYYDNLTCNFKLSTPSDVSLTTQKLNT
IIHWYTHDALIHFASPHGLEQSGGAAWGTRDVCQGPIELFLTTQHYPLVRDILITLFSHQ
FDEIFEWPQWFMFDRYPMHQEDCHGDIVFWPLKAISDYIQATGDSSILEEVVDYRTQKGA
LPVNKPETILTHIKHSVDAIKKRYLPGTALISYAGGDWDDTLQPANRKLKENLVSAWTQA
LAMQTLELLSLSVLDTDESFSKDVSHMATEIKNSFYQYLVKDGVIAGFLYRESEDHMKYM
LHPFDTETSIHYRLLPLTRSIIAQLAEPELAARNMDIIDQHLTCPDGVRLMDHPASYNGG
ISKIFLRAEQAANVGREISLQYSHAHIRYIEAAATLGLSAKAWDAVMRINPILLTEYVPN
ALTRQSNVYFSSSEGCFNDRYEYAANFDKLRTGDISVKGGWRLYSSGPGIYIRRIIADLL
GIRFTNNKIHIDPVITEDFDGASLQYTCYGKTVSFIYHIDNSMDQLICVKSRNKELPGNY
TMNQYRNSGFEISKDDFIEASKEDNQFHIYLKK

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH94	94	1064	1.2e-157	0.944015444015444

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3459	COG3459	4.33e-167	120	1065	117	1021	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
pfam17167	Glyco_hydro_36	1.08e-12	575	952	1	370	Glycosyl hydrolase 36 superfamily, catalytic domain. This is the catalytic region of the superfamily of enzymes referred to as GH36. UniProtKB:Q76IQ9 is a chitobiose phosphorylase that catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. The full-length enzyme comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain. The alpha-helical barrel component of the domain, this family, is the catalytic region.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABX41081.1	0.0	1	1112	1	1112
AUS95384.1	0.0	10	1111	10	1115
AIQ43298.1	0.0	10	1111	2	1106
AIQ60493.1	0.0	1	1111	1	1114
AIQ20111.1	0.0	10	1111	2	1105

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5H3Z_A	0.0	2	1112	3	1113	CrystalStructure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans [Lachnoclostridium phytofermentans ISDg],5H3Z_B Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans [Lachnoclostridium phytofermentans ISDg],5H40_A Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with sophorose [Lachnoclostridium phytofermentans ISDg],5H40_B Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with sophorose [Lachnoclostridium phytofermentans ISDg]
5H41_A	0.0	2	1112	3	1113	CrystalStructure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with sophorose, isofagomine, sulfate ion [Lachnoclostridium phytofermentans ISDg],5H41_B Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with sophorose, isofagomine, sulfate ion [Lachnoclostridium phytofermentans ISDg],5H42_A Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with alpha-d-glucose-1-phosphate [Lachnoclostridium phytofermentans ISDg],5H42_B Crystal Structure of 1,2-beta-oligoglucan phosphorylase from Lachnoclostridium phytofermentans in complex with alpha-d-glucose-1-phosphate [Lachnoclostridium phytofermentans ISDg]
5NZ7_A	1.56e-06	680	1059	574	965	ChainA, Cellodextrin phosphorylase [Acetivibrio thermocellus],5NZ7_B Chain B, Cellodextrin phosphorylase [Acetivibrio thermocellus],5NZ8_A Chain A, Cellodextrin phosphorylase [Acetivibrio thermocellus],5NZ8_B Chain B, Cellodextrin phosphorylase [Acetivibrio thermocellus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q92AT0	2.15e-254	12	1041	7	1017	1,2-beta-oligoglucan phosphorylase OS=Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262) OX=272626 GN=lin1839 PE=1 SV=1
P20471	8.83e-07	567	1052	2336	2800	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.998516	0.001448	0.000034	0.000007	0.000003	0.000017

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003769_00998.