CAZyme Information: MGYG000003794_00343

Basic Information

• Species: Prevotella timonensis
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella timonensis
• CAZyme ID: MGYG000003794_00343
• CAZy Family: GH20
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1211	MGYG000003794_8|CGC1	135732.49	5.7362

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003794	2491297	MAG	Canada	North America

• Gene Location: Start: 7192; End: 10827 Strand: -

Full Sequence      

MPFFCVHIKFKEMPFMLFQFDSRHLSLIFNPMKKTLYTILFLLLGSLCNLQAADFPKIST
ADNTFWYFIQFANSQNVLTSQGDGVKVQTHSMTGQPAQLWKIEGSESAGFTLTDKTGRHL
YLNSTAKQGMAFASSKHQATKLKIKSLPSGKYEVTPYDNGAVALNQWTGPGVGHEVGLWD
VGDVNNPLSFTPESALEAYRNLPRLIPYPSSLTLVEGETYELSQMHSIAYTNDVTQQQAT
EFANQLAATAGIQLNVEPCTTDRKENAISVVIDNTLPDEGYTLMVDAKGVLIKTKTDAGY
FYALQTIKQLLPNAFYEKNPVSGAIWTLPQLFINDAPALDHRGFMLDVARHFFDKHEVMR
ILDIMAFYKMNRFHWHLTDDQGWRVEIPEYPKLTTVGSKRAGSFVNAGGSQQFFDDTEYG
EGMYYTLDDLREVVKYAQERNITIIPEIDLPGHMVAAVAAYPELSCDPTKTYRVRVPGGI
SKDVLNIGKDSTIDFLKCVLGHVATVFPGPYIHLGGDECPTDQWRNNADCLERVRAEGLS
GVEQLQSWLVELLGEYLYDNYGKRIIVWDELMAHWPATNKVKPVIMAWNHINKSAEAADL
GFKSIVVPYQSLYLDMMQVPKSQADVNEIYQGGWGDGYVNSVPTVYSVNPVSALSGREDY
CLGVQGNMWTETCNDNTQLEYQLLPRLLALAETGWLPESKKKWVSFYQRLQKHDELLDRM
EYVYARHYIEANQDALQTAIQEAQTLLDTTTPGEAGYVSAEEHQTLSETLQKAKDGTADV
QVLGNAIAHFKAADVVQPQENEVYQIVSASTYYKKRYVGSTMYQNQQGVRFHYTPQNEPE
ELWQFVKTGKGWKIRNAVTQQYIQLADYNKAVRMNATEATVFHVDKATVPAKQYDYVAGA
LVMTNAANYSATATGSTPRLFGHPSGDVVSFDDPMLCNPGTWRLVRITQYKPLLEALVKK
CDKLIAGNVPHQPGSFTDEAVSFLNAKVIVPAHAHLADGKAVSHAEYMEYAALYQQFLSI
PRTSIVESLDEAYWYKIRNGYFTDYYAKLNPADNNVYPVRTTATTDDLLWRFVKNADGTV
GIISRVGEVPACVENSVIDQTIHAGTTYSWKLSPYTTDEGPSGFNIIEASGAYSWYTNPD
AFQTVLLKPRYWGASIWTFEKQAEKVTSIPHTAADATQEAYYNLQGVKVGTDASKLQQGV
YLCNGKKVVVR

Enzyme Prediction     

EC	3.2.1.52

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH20	339	696	3e-106	0.9554896142433235

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06563	GH20_chitobiase-like	2.99e-153	341	710	3	357	The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. This GH20 domain family includes an N-acetylglucosamidase (GlcNAcase A) from Pseudoalteromonas piscicida and an N-acetylhexosaminidase (SpHex) from Streptomyces plicatus. SpHex lacks the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
pfam00728	Glyco_hydro_20	1.39e-128	341	695	3	343	Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.
COG3525	Chb	4.16e-90	275	778	202	680	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].
cd06568	GH20_SpHex_like	7.15e-86	340	710	2	329	A subgroup of the Glycosyl hydrolase family 20 (GH20) catalytic domain found in proteins similar to the N-acetylhexosaminidase from Streptomyces plicatus (SpHex). SpHex catalyzes the hydrolysis of N-acetyl-beta-hexosaminides. An Asp residue within the active site plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself. Proteins belonging to this subgroup lack the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases.
cd06562	GH20_HexA_HexB-like	1.33e-73	341	717	3	344	Beta-N-acetylhexosaminidases catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. The hexA and hexB genes encode the alpha- and beta-subunits of the two major beta-N-acetylhexosaminidase isoenzymes, N-acetyl-beta-D-hexosaminidase A (HexA) and beta-N-acetylhexosaminidase B (HexB). Both the alpha and the beta catalytic subunits have a TIM-barrel fold and belong to the glycosyl hydrolase family 20 (GH20). The HexA enzyme is a heterodimer containing one alpha and one beta subunit while the HexB enzyme is a homodimer containing two beta-subunits. Hexosaminidase mutations cause an inability to properly hydrolyze certain sphingolipids which accumulate in lysosomes within the brain, resulting in the lipid storage disorders Tay-Sachs and Sandhoff. Mutations in the alpha subunit cause in a deficiency in the HexA enzyme and result in Tay-Sachs, mutations in the beta-subunit cause in a deficiency in both HexA and HexB enzymes and result in Sandhoff disease. In both disorders GM(2) gangliosides accumulate in lysosomes. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFQ12497.1	2.49e-222	268	945	73	712
QFQ12789.1	7.09e-192	32	1019	1	955
BBL09404.1	1.09e-115	205	804	26	609
BBL01546.1	1.09e-115	205	804	26	609
BBL12197.1	1.09e-115	205	804	26	609

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7DUP_A	3.75e-106	205	730	6	526	ChainA, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron],7DVA_A Chain A, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron],7DVA_B Chain B, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron]
7DVB_A	1.93e-105	205	730	6	526	ChainA, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron],7DVB_B Chain B, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron],7DVB_C Chain C, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron],7DVB_D Chain D, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron]
6Q63_A	4.50e-97	204	734	30	546	BT0459[Bacteroides thetaiotaomicron],6Q63_B BT0459 [Bacteroides thetaiotaomicron],6Q63_C BT0459 [Bacteroides thetaiotaomicron]
3RCN_A	4.86e-76	264	710	63	498	CrystalStructure of Beta-N-Acetylhexosaminidase from Arthrobacter aurescens [Paenarthrobacter aurescens TC1]
7CBN_A	5.86e-76	203	726	11	512	Crystalstructure of beta-N-acetylhexosaminidase Am0868 from Akkermansia muciniphila [Akkermansia muciniphila ATCC BAA-835],7CBO_A Crystal structure of beta-N-acetylhexosaminidase Am0868 from Akkermansia muciniphila in complex with GlcNAc [Akkermansia muciniphila ATCC BAA-835]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P49008	1.12e-98	205	718	37	526	Beta-hexosaminidase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=nahA PE=3 SV=2
B2UQG6	4.23e-75	203	726	30	531	Beta-hexosaminidase Amuc_0868 OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_0868 PE=1 SV=1
P96155	2.03e-64	205	694	141	604	Beta-hexosaminidase OS=Vibrio furnissii OX=29494 GN=exoI PE=1 SV=1
B2UP57	1.41e-61	242	722	15	475	Beta-hexosaminidase Amuc_2018 OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_2018 PE=1 SV=1
Q04786	5.90e-48	268	714	254	740	Beta-hexosaminidase OS=Vibrio vulnificus OX=672 GN=hex PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.572790	0.424585	0.001794	0.000284	0.000232	0.000308

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003794_00343.