CAZyme Information: MGYG000003808_01003

Basic Information

• Species: Pauljensenia culturomici
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Pauljensenia; Pauljensenia culturomici
• CAZyme ID: MGYG000003808_01003
• CAZy Family: GH116
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
842	MGYG000003808_40|CGC1	93434.53	4.8773

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003808	2368539	MAG	Canada	North America

• Gene Location: Start: 2474; End: 5002 Strand: +

Full Sequence      

MVHSFGKHGSEEFGDVSRPIYSSSDTTWRFPLGGVCTGGVSFGPRCELTDWQLWNGPRFG
GKNGYSFFAIRTTNPIGIPTSRIIESQYLAPFDWQGGSPFAELAGLPRFTTGTVSALFPF
AFGELADPEFPASVHIRAVTPFIPLDLDSSSLPLVEFRISVENTSATPLSFSVVGTTSYD
PSLFADTVDSGLAAPCSTVIREHGWSGVELAGAASGGKYSSIALAIGDEGAEVLPAWSVD
FWPDASEQFWTEFTTTGRLPSEGLPTHESEPLPLFSSNFDPAHHDIWDVLPKERTASVAS
TQEIAPGDIGEFVFVLAWLVPYRLAEWNGNIGLEGGETHRRVLNSYAERWANAHEVIAHY
FGHRDDLLTSSSRFAVTLCDSTIPAASQRSIAASLAALRTPLVFAIEDGARQGPVLGAWE
GLFESVGSCEGTCTHVWSYAQTLAWVFPELERSARELEFHEETSPEGSQRFRSNALFRNP
QWFMAPAVDGQLGTILRLYREWRFAGDDTWLSRRWYSAARALDFALTNWVDPETGLVNAR
VHNTYDIEFEGSEPLANSMLIAALDAGERLARRMHDTIHADTWKEARLRCRTAFGELLFN
GEYFQQIGCSEAARHQFGSGVLSDQLLGQFYALVNGLEQITNNASLDSALDAIFTHNFVA
DLSCVPSTRRAFALPREAGLLLASWPHGDRPQVPFVYSDEVWTGIEHEVATLLLSRGRIA
QCNRIESAISERYDGVKRNPWSEIECGRYYARSMSSWALLLTESGQQWDAPHRRLDFHPA
RPDGYSGLFITNSAWGRVTINPNEFKIRVLQGTLDKVTIVCNGYVLWRNITLECEQYLHA
FR

Enzyme Prediction     

No EC number prediction in MGYG000003808_01003.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH116	486	759	1.9e-50	0.7327823691460055

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam04685	DUF608	3.66e-34	489	759	99	362	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.
pfam12215	Glyco_hydr_116N	9.10e-34	30	367	2	309	beta-glucosidase 2, glycosyl-hydrolase family 116 N-term. This domain is found in bacteria, archaea and eukaryotes. This domain is typically between 320 to 354 amino acids in length. This domain is found associated with pfam04685. It is found just after the extreme N-terminus. The N-terminal is thought to be the luminal domain while the C terminal is the cytosolic domain. The catalytic domain of GBA-2 is unknown. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335 in the downstream family pfam04685.
COG4354	COG4354	5.53e-23	21	765	5	719	Uncharacterized protein, contains GBA2_N and DUF608 domains [Function unknown].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QEW04614.1	2.74e-225	30	823	30	834
AZS42112.1	4.61e-224	17	833	7	837
QAY61718.1	2.14e-222	26	838	12	832
AZH80064.1	1.33e-220	30	836	19	840
QBR90387.1	1.75e-219	30	823	17	821

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q69ZF3	9.76e-08	31	767	144	885	Non-lysosomal glucosylceramidase OS=Mus musculus OX=10090 GN=Gba2 PE=1 SV=2
Q5M868	1.28e-07	31	784	144	898	Non-lysosomal glucosylceramidase OS=Rattus norvegicus OX=10116 GN=Gba2 PE=2 SV=2
Q9HCG7	7.68e-06	31	767	153	894	Non-lysosomal glucosylceramidase OS=Homo sapiens OX=9606 GN=GBA2 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.998781	0.001110	0.000098	0.000005	0.000002	0.000026

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003808_01003.