CAZyme Information: MGYG000003815_00483

Basic Information

• Species: Succiniclasticum sp900314855
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Acidaminococcales; Acidaminococcaceae; Succiniclasticum; Succiniclasticum sp900314855
• CAZyme ID: MGYG000003815_00483
• CAZy Family: GH23
• CAZyme Description: Soluble lytic murein transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
225		25882.95	10.2397

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003815	1813778	MAG	United States	North America

• Gene Location: Start: 32282; End: 32959 Strand: +

Full Sequence      

MLGRKKKRSRKTIRPWGLLSGHHRNGRNRKGLSGLLAHGKLLAVVLVLAGFLGWTAWRSD
YVQMRFVYLWPYQEEIVTYSHKNNVDPFLTAAVIKNESGFKPGAVSRVGAVGLMQIMPDT
GEWIAGQMGLKQYSFAALYQPETNIRMGCWYLSELHHEFKGNMVLMLIAYNAGRGNTHVW
MKANGWDYHYGNVAAIPYAESREYVAKVLHDRDEYYRLYKDKIPR

Enzyme Prediction     

No EC number prediction in MGYG000003815_00483.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH23	85	213	1.3e-30	0.8

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd16896	LT_Slt70-like	2.14e-77	70	213	2	145	uncharacterized lytic transglycosylase subfamily with similarity to Slt70. Uncharacterized lytic transglycosylase (LT) with a conserved sequence pattern suggesting similarity to the Slt70, a 70kda soluble lytic transglycosylase which also has an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
cd13401	Slt70-like	3.12e-55	68	220	2	150	70kDa soluble lytic transglycosylase (Slt70) and similar proteins. Catalytic domain of the 70kda soluble lytic transglycosylase (LT)-like proteins, which also have an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria and the LTs in bacteriophage lambda.
cd00254	LT-like	1.70e-37	87	209	1	107	lytic transglycosylase(LT)-like domain. Members include the soluble and insoluble membrane-bound LTs in bacteria and LTs in bacteriophage lambda. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
COG0741	MltE	1.19e-31	71	225	138	289	Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains) [Cell wall/membrane/envelope biogenesis].
pfam01464	SLT	1.47e-29	82	186	7	108	Transglycosylase SLT domain. This family is distantly related to pfam00062. Members are found in phages, type II, type III and type IV secretion systems.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTV76946.1	1.22e-90	24	223	5	205
BBG64618.1	5.84e-83	44	225	19	200
QNP77119.1	9.76e-82	44	225	30	211
AEQ23289.1	3.73e-66	38	219	15	193
ADB46849.1	2.54e-64	41	220	17	193

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MPQ_A	1.97e-25	68	219	412	559	BulgecinA: The key to a broad-spectrum inhibitor that targets lytic transglycosylases [Neisseria meningitidis]
5O1J_A	1.99e-25	68	219	416	563	Lytictransglycosylase in action [Neisseria meningitidis MC58]
6FPN_B	2.01e-25	68	219	422	569	Lytictransglycosylase in action [Neisseria meningitidis MC58]
5O24_A	2.03e-25	68	219	426	573	Lytictransglycosylase in action [Neisseria meningitidis]
5O29_A	2.05e-25	68	219	432	579	Lytictransglycosylase in action [Neisseria meningitidis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39434	4.53e-17	60	217	470	626	Soluble lytic murein transglycosylase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=slt PE=3 SV=2
P0AGC3	6.14e-17	60	224	470	633	Soluble lytic murein transglycosylase OS=Escherichia coli (strain K12) OX=83333 GN=slt PE=1 SV=1
P0AGC4	6.14e-17	60	224	470	633	Soluble lytic murein transglycosylase OS=Escherichia coli O157:H7 OX=83334 GN=slt PE=3 SV=1
O31976	3.43e-16	68	209	1418	1538	SPbeta prophage-derived uncharacterized transglycosylase YomI OS=Bacillus subtilis (strain 168) OX=224308 GN=yomI PE=3 SV=2
O64046	3.43e-16	68	209	1418	1538	Probable tape measure protein OS=Bacillus phage SPbeta OX=66797 GN=yomI PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999954	0.000041	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
35	57