CAZyme Information: MGYG000003816_00103

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-485
• CAZyme ID: MGYG000003816_00103
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
846	MGYG000003816_4|CGC1	96013.29	5.6949

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003816	2849186	MAG	United States	North America

• Gene Location: Start: 2415; End: 4955 Strand: +

Full Sequence      

MNLKPLLLSLASLASIHAVALDNPGITFPTLDQARFTLIAQGSPATILIADDEDTAVKIA
AANLARDFGRVCGKDAAVSSTATSMSRPVIATTVGTRTAEAILKKGLVDRKEIKGKREKY
IITTVKNPVQGVEEALLIIGSDRRGTVYGIYELSEQIGVSPWYDWADVPVGKHSDLSIEK
GVYTAGEPAVRYRGIFLNDEAPCLTSWVKNTYGTDYGDHNFYARVNELILRLRGNFLWPA
MWGWAFYADDPDNSRTADEMGIIMGTSHHEPMARNHQEWARHRNEYGKWNYAANKKTIDG
FFRKGIERMKDTEDIVTIGMRGDGDEAMSEETDVKLLRDIIDNQRKIIREVTGKKPEKTP
QVWALYKEVLDYYDKGLRAPDDVTYLLCDDNWGNVRRLPDAEERKHPGGWGMYYHVDYVG
APRNSKWINVTPVQNMWEQLNLTYDYGVDRLWVLNVGDLKPMEYPITLFLDMAWNPKRYD
AAGILRHTEDFCRQQFGETQAAEAARIINLYSKYNGRVTAEMLDADTYDLPSGEWKRVAD
DYARLEAEALRQFLTLRPEQRDAYRQLILFPVQAMANLYEMYYSQAMNRHLFDNGDPDAD
LWADAVERCFRRDRELERQYNKEMSAGKWDGMMTQKHIGYKEWHDGFKEDTMPEVRRIGV
SAPGGFTFSPSASGVVMEAEHYHDATTGTSTSWTVYPHMGRTRSGVALTPYTERPAGERI
SYRFEYPQMPDSVDVRVVKSTLAFANPEGHSFSVGFEGEEPVTVNFNSDLNEKPENIYSV
FYPTVARRVVSKNVRLRKPASASGDVHTLSLRPLDPGIVFEKIIVGPAALSDEYLFGTES
ECRKVR

Enzyme Prediction     

EC	3.2.1.131

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	34	823	3.4e-269	0.9971305595408895

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	0.0	186	522	1	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	2.08e-26	672	839	5	172	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam03648	Glyco_hydro_67N	1.38e-06	41	153	18	120	Glycosyl hydrolase family 67 N-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the N-terminal region of alpha-glucuronidase. The N-terminal domain forms a two-layer sandwich, each layer being formed by a beta sheet of five strands. A further two helices form part of the interface with the central, catalytic, module (pfam07488).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCD34650.1	0.0	3	844	4	846
ABR37760.1	0.0	10	844	11	858
QQY39902.1	0.0	10	844	11	858
QUT78586.1	0.0	12	844	11	842
QDM11119.1	0.0	12	844	11	842

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4C90_A	0.0	12	844	22	853	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]
7PUG_A	0.0	22	845	3	839	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
7PXQ_A	0.0	22	845	2	838	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
4ZMH_A	8.56e-171	45	655	23	630	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
6NPS_A	5.23e-116	107	645	84	647	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000294	0.998982	0.000177	0.000192	0.000167	0.000159

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003816_00103.