CAZyme Information: MGYG000003821_00937

Basic Information

• Species: Blautia_A sp900549015
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia_A; Blautia_A sp900549015
• CAZyme ID: MGYG000003821_00937
• CAZy Family: GH3
• CAZyme Description: Thermostable beta-glucosidase B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
783		87059.05	5.3831

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003821	2665084	MAG	United States	North America

• Gene Location: Start: 41792; End: 44143 Strand: +

Full Sequence      

MEVKDIISQLTIEEKAALLSGKNEWESRDIPRLGIPSITFSDGPHGLRRQEGAGDHLGLN
ESLPATCFPTAASTANSWDPALGEKIGEALGKEAVAQGVNVVLGPGLNMKRSPLCGRNFE
YFSEDPVLAGKMAAAYVRGIQSRHAYACPKHFAVNSREYRRMAMNAVVDERTLREIYLTA
FEIAVKEGKAKSIMSAYNEVNGKYANENEHLLIDILRKEWGFDGIVVTDWGASNNHTEGV
KCQSNVEMPNPGLDSARGLLEDLKLGKITEAEIDRAIEPIIEASIYFSKNEQKDFDKEAH
HRLARRAAAESAVLLKNEKGILPLKEGTKVALKGPFVNKPRYQGSGSSQVNATKVETVAE
EIRNYNLEVVEDPKEAEVVLFFFGLDEIAESEGADRTSMDIPEYQIEELNKLAVLNSNIV
GVMSAGSAVKMPWLNKLTALLHGYLTGQAGASALLDLLTGKEVPTGKLAESYPFAYADCS
MFNYADEKYRNLQYREGPFIGYRYYDTAGVAVQFPFGYGLSYTTFSYDSLVLTDTGVRFT
ITNTGMRAGAEIAQMYVGKQESGLVRPKKELKGFAKVWLEAGESREVEIEFDDKTFRYFN
TKTNQWEVEGGIYTVMIGASSADIKLTAELEKQGTTEVLPYDIEKLPNYKAGLVRNVHYE
EFQIFYGDVLPEEKVGPLDVNDALCQMKDAKNGLCRFIYKVLQKRLDKSVEKGVPDLNTL
FIYNMPFRAICKMTGGMVSREMTESMVTAANGHFFRGLAGVIGGFFRNMRENKKYETLLK
NEK

Enzyme Prediction     

EC	3.2.1.21	3.2.1.-	3.2.1.45

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	29	254	1.9e-68	0.9675925925925926

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	2.63e-83	32	625	101	758	beta-glucosidase BglX.
COG1472	BglX	7.38e-70	30	354	55	377	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
PLN03080	PLN03080	3.91e-61	6	592	56	743	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	1.57e-41	312	522	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
pfam00933	Glyco_hydro_3	4.16e-37	32	283	63	316	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFJ55981.1	0.0	1	779	1	782
CBK75766.1	0.0	1	781	1	782
QRT31068.1	0.0	1	779	1	807
QEI32524.1	0.0	1	779	1	807
QHB25014.1	0.0	1	779	1	807

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7MS2_A	1.61e-197	1	751	3	752	ChainA, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2],7MS2_B Chain B, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2]
5WAB_A	2.73e-167	7	642	9	673	CrystalStructure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_B Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_C Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_D Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703]
2X40_A	2.92e-119	2	626	3	707	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with glycerol [Thermotoga neapolitana DSM 4359],2X41_A Structure of beta-glucosidase 3B from Thermotoga neapolitana in complex with glucose [Thermotoga neapolitana DSM 4359]
2X42_A	4.38e-118	2	626	3	707	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with alpha-D-glucose [Thermotoga neapolitana DSM 4359]
3AC0_A	1.86e-97	2	631	5	835	Crystalstructure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_B Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_C Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_D Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P14002	4.42e-197	1	751	3	752	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2
F6C6C1	7.65e-162	7	639	9	681	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium breve (strain ACS-071-V-Sch8b) OX=866777 GN=HMPREF9228_1477 PE=1 SV=1
E7CY69	1.20e-160	7	640	9	680	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium longum OX=216816 GN=apy PE=1 SV=1
A7LXS8	2.24e-123	1	631	44	744	Beta-glucosidase BoGH3A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02644 PE=1 SV=1
Q5BFG8	1.14e-105	2	633	10	841	Beta-glucosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bglB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003821_00937.