CAZyme Information: MGYG000003825_01331

Basic Information

• Species: UBA1777 sp900549645
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; UBA1777; UBA1777 sp900549645
• CAZyme ID: MGYG000003825_01331
• CAZy Family: GH20
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1661		181485.32	4.3013

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003825	2022533	MAG	United States	North America

• Gene Location: Start: 6900; End: 11885 Strand: +

Full Sequence      

MNRTRTSKNAKRLLVWLLLLTMVFGLLPMAAFADETVVEPVVSEEPVQETEPSSESTEAP
SEPTQEPTQEPEPEQPGNAVVTIEGDEEQPEDEGNDEVILDDYWLYPQPMGISELASTNG
FMKIFFLDCGRKYFSVDNIKALIDNASAAGFNYIQLAVGNDGLRFLLDDMSLTVNGKDYT
TEQVSNAIKNGNKNYHNFTVNELTQTEMDTIITYATKKGMGVIPCVNTPGHMDAILSAAS
SLTGTDCSYSGSARTIDVTNATAVEFTQALLQKYIDYFKGKDCQFFNMGADEYANDIYTG
GSMGFGNLQSTGKYSYYVQYVNQVADKIIKAGMTPMAFNDGIYFNNNTQSGSFNTGILIC
YWSSGWSSYSPMSASALKENGFSLVNTHGDYYWVLGKSDAQCSPAKAAGFDKTAFPGGTI
SDPSGAMFCIWCDYPGADTAENVVSKTAATIDAFGKALPNPPTTGGGSTGGGSTGGETQE
PVPVNLTVGETSDHYYQDGDYSSVKNTDDDTIATVVTSVGDKPGKTTYESTKIGEGTFWI
STSPSTTTEPPVKLTFESVGNNECYVKNAGGQYIYPEKYWAMNGYKARVQTSDNPVPVSV
SANGDGNYRFSYYRNYFGSAYLVYDGSTLSASPDSGTSLYLLEEKTTPAEKQTTITFTGV
KPGTTTCQIGNTKYTITVTEEDLKKVTPLELEYWITNGRVKDSSTSETTTEVSALTDGIA
TSGGVDASTLAASPGNKDGRDVFYWQCRLLDTTKANSSTSGTERQTEQGGDDDTSSGVGF
TKIRYYNAAWAVYTENFEWVAVESNHQLVAYYREYIKVSDEIESYAADWGNRGDGTPGGW
LDTSSYCTLSMQVVYEDNTTNPISTSAAALKSKTIVYGYWSNEGGRGIGTVMLDGKEYEI
YKVTSETGAATVSFSGYNATVANFTWDNNETTVWEGDATDSVTIHNPSKGYSTDGANANL
CWDENKEAILLRVYIRAKVTEDSLTVHYVETQNNQDFYSYNIAVAQNTVFDAGFAKQGDG
LVNNTVVNIKGQTQTVKSNLSEMQEVGAQYKYANYTFDKAVRSQDGKEVFLYYSFTRNKS
FVVDFGLPLEIKPTDLSDTLASANIEKTVAESTSFAKITVNNPNIKYELIKTLDQNDIFS
VNYSGTNLQTSSPDGINFTIEVIPATSVYYEDSFAKFANGKNTASGAKWSTVGTEDVNAT
QALSAIGDKNSDVYGYDNAYDKCSTFSLGSAQMVTVNSNMLTNWDANSAWPTATFTFKGT
GFDIISLTNNQSGTIFVDVVRTSDNQKVKGYIVDNYYGYKQENGTWVVDPSRTDTLYQIP
VIKASGLDYNEYTVTVKVVYGAAADHTDKGSYSFWFDAFRVYNPMGLENKYIEEYVKDNE
GYPQYIKLRDQLLNNSASARQMLFIDGAANASVADYKNFGPNNEVYLAAGQAISFTINGE
SVKSIQIGAKAPNGAAVLNVNGADIKTLDTATEMYYDITEQATKGSYKFTITNRGNNILS
LTNIKITFDSNATASLAELGEDDAVQAVSLVRSLYAAPVVEPVEPFKPEHFEAKWSNNVR
QGGRAVLTVKTSADVEQIIINGDIVVSNYKTRTERVGYGWNAERVTYHVFTYMISAQQSA
EYSVVAVNEAGLMSEAVTARLTVRPKGPIKDWFDGLFGRWF

Enzyme Prediction     

EC	3.2.1.52	3.2.1.96

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH20	125	439	1.9e-40	0.884272997032641

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06564	GH20_DspB_LnbB-like	1.29e-61	118	441	2	305	Glycosyl hydrolase family 20 (GH20) catalytic domain of dispersin B (DspB), lacto-N-biosidase (LnbB) and related proteins. Dispersin B is a soluble beta-N-acetylglucosamidase found in bacteria that hydrolyzes the beta-1,6-linkages of PGA (poly-beta-(1,6)-N-acetylglucosamine), a major component of the extracellular polysaccharide matrix. Lacto-N-biosidase hydrolyzes lacto-N-biose (LNB) type I oligosaccharides at the nonreducing terminus to produce lacto-N-biose as part of the GNB/LNB (galacto-N-biose/lacto-N-biose I) degradation pathway. The lacto-N-biosidase from Bifidobacterium bifidum has this GH20 domain, a carbohydrate binding module 32, and a bacterial immunoglobulin-like domain 2, as well as a YSIRK signal peptide and a G5 membrane anchor at the N and C termini, respectively. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
cd02742	GH20_hexosaminidase	4.55e-21	125	438	4	283	Beta-N-acetylhexosaminidases of glycosyl hydrolase family 20 (GH20) catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. These enzymes are broadly distributed in microorganisms, plants and animals, and play roles in various key physiological and pathological processes. These processes include cell structural integrity, energy storage, cellular signaling, fertilization, pathogen defense, viral penetration, the development of carcinomas, inflammatory events and lysosomal storage disorders. The GH20 enzymes include the eukaryotic beta-N-acetylhexosaminidases A and B, the bacterial chitobiases, dispersin B, and lacto-N-biosidase. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by the solvent or the enzyme, but by the substrate itself.
COG3525	Chb	2.21e-17	126	353	16	244	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].
cd06565	GH20_GcnA-like	6.51e-12	122	340	1	186	Glycosyl hydrolase family 20 (GH20) catalytic domain of N-acetyl-beta-D-glucosaminidase (GcnA, also known as BhsA) and related proteins. GcnA is an exoglucosidase which cleaves N-acetyl-beta-D-galactosamine (NAG) and N-acetyl-beta-D-galactosamine residues from 4-methylumbelliferylated (4MU) substrates, as well as cleaving NAG from chito-oligosaccharides (i.e. NAG polymers). In contrast, sulfated forms of the substrate are unable to be cleaved and act instead as mild competitive inhibitors. Additionally, the enzyme is known to be poisoned by several first-row transition metals as well as by mercury. GcnA forms a homodimer with subunits comprised of three domains, an N-terminal zincin-like domain, this central catalytic GH20 domain, and a C-terminal alpha helical domain. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
COG3525	Chb	8.98e-11	127	434	268	590	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALP95510.1	3.10e-184	3	1528	2	1703
QBB67228.1	5.68e-166	3	1528	2	1706
QQX68397.1	2.02e-80	116	437	32	356
QQX71249.1	2.02e-80	116	437	32	356
QPI88500.1	2.02e-80	116	437	32	356

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7PUL_A	1.69e-78	116	438	4	329	ChainA, Beta-N-acetylhexosaminidase [Enterococcus faecalis]
4AZ7_A	3.02e-67	123	437	8	343	Differentialinhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH [Streptococcus pneumoniae TIGR4]
2YL6_A	3.10e-67	123	437	8	343	Inhibitionof the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis [Streptococcus pneumoniae TIGR4],4AZ5_A Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH [Streptococcus pneumoniae TIGR4]
4AZB_A	3.10e-67	123	437	8	343	Differentialinhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH [Streptococcus pneumoniae TIGR4]
4AZ6_A	3.19e-67	123	437	10	345	Differentialinhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P49610	2.32e-61	123	437	188	523	Beta-N-acetylhexosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=strH PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000448	0.998820	0.000229	0.000173	0.000160	0.000145

TMHMM  Annotations     

start	end
13	32