CAZyme Information: MGYG000003837_01397

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Porphyromonadaceae; Porphyromonas
• CAZyme ID: MGYG000003837_01397
• CAZy Family: GH23
• CAZyme Description: Membrane-bound lytic murein transglycosylase F

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
336	MGYG000003837_33|CGC1	38410.84	5.297

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003837	2173953	MAG	United States	North America

• Gene Location: Start: 622; End: 1632 Strand: +

Full Sequence      

MKCSRRDEIRRDPTRMRRLLRTITLSLWGAVLVGVVYYFAVVYQAPQAQTARTHTTSQTE
WHDVPIADVGAGEAYEELERYSTLYALEYLLDCDSIWPNVEANRLQLFDLYEEEEDDEDD
EEEVVQVKVVPAKPASTSETSQEQSQAQSTRRQGAISDWDYLFQQYGNKYGWDWYVLAAI
AYQESKFRAEVVGMGGATGLMGIMPATGRRYGYSRAKLKHAESSIRIACMALRDFGRAFA
HITDAEQRMKFTLASYNSGSAHVLDARRLAEDAGLDPDRWDDSVEIYMARLNEPKYYNHP
LVQHGRANGAHTVRYVAEVFNRAQSYKSKVQYATTE

Enzyme Prediction     

No EC number prediction in MGYG000003837_01397.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH23	171	332	1.1e-20	0.8666666666666667

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd13403	MLTF-like	2.09e-64	163	327	1	160	membrane-bound lytic murein transglycosylase F (MLTF) and similar proteins. This subfamily includes membrane-bound lytic murein transglycosylase F (MltF, murein lyase F) that degrades murein glycan strands. It is responsible for catalyzing the release of 1,6-anhydromuropeptides from peptidoglycan. Lytic transglycosylase catalyzes the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc) as do goose-type lysozymes. However, in addition, it also makes a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
COG4623	MltF	1.77e-27	159	319	271	424	Membrane-bound lytic murein transglycosylase MltF [Cell wall/membrane/envelope biogenesis, Signal transduction mechanisms].
PRK10859	PRK10859	1.21e-24	158	317	289	441	membrane-bound lytic murein transglycosylase MltF.
cd16896	LT_Slt70-like	5.12e-13	164	278	9	121	uncharacterized lytic transglycosylase subfamily with similarity to Slt70. Uncharacterized lytic transglycosylase (LT) with a conserved sequence pattern suggesting similarity to the Slt70, a 70kda soluble lytic transglycosylase which also has an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
pfam01464	SLT	1.03e-12	163	270	1	106	Transglycosylase SLT domain. This family is distantly related to pfam00062. Members are found in phages, type II, type III and type IV secretion systems.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEE12384.1	1.60e-186	1	336	1	331
BAK25352.1	1.74e-50	151	329	291	471
AUR47574.1	1.74e-50	151	329	291	471
AKV63991.1	4.75e-50	152	329	292	471
ALO29436.1	4.75e-50	152	329	292	471

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OZ9_A	2.38e-14	163	319	263	414	Crystalstructure of MltF from Pseudomonas aeruginosa complexed with isoleucine [Pseudomonas aeruginosa PAO1]
4OYV_A	2.43e-14	163	319	270	421	Crystalstructure of MltF from Pseudomonas aeruginosa complexed with leucine [Pseudomonas aeruginosa PAO1]
4OWD_A	2.43e-14	163	319	270	421	Crystalstructure of MltF from Pseudomonas aeruginosa complexed with cysteine [Pseudomonas aeruginosa PAO1],4OXV_A Crystal structure of MltF from Pseudomonas aeruginosa complexed with valine [Pseudomonas aeruginosa PADK2_CF510],4P0G_A Crystal structure of MltF from Pseudomonas aeruginosa complexed with bulgecin and muropeptide [Pseudomonas aeruginosa PAO1],4P11_A Native crystal structure of MltF Pseudomonas aeruginosa [Pseudomonas aeruginosa PAO1]
5AA4_B	3.40e-14	163	319	256	407	Crystalstructure of MltF from Pseudomonas aeruginosa in complex with cell-wall tetrapeptide [Pseudomonas aeruginosa BWHPSA013],5AA4_D Crystal structure of MltF from Pseudomonas aeruginosa in complex with cell-wall tetrapeptide [Pseudomonas aeruginosa BWHPSA013]
5AA4_A	3.40e-14	163	319	256	407	Crystalstructure of MltF from Pseudomonas aeruginosa in complex with cell-wall tetrapeptide [Pseudomonas aeruginosa BWHPSA013],5AA4_C Crystal structure of MltF from Pseudomonas aeruginosa in complex with cell-wall tetrapeptide [Pseudomonas aeruginosa BWHPSA013]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0AQY9	5.70e-18	162	319	292	444	Membrane-bound lytic murein transglycosylase F OS=Maricaulis maris (strain MCS10) OX=394221 GN=mltF PE=3 SV=2
A1U3J1	2.95e-17	162	319	289	441	Membrane-bound lytic murein transglycosylase F OS=Marinobacter nauticus (strain ATCC 700491 / DSM 11845 / VT8) OX=351348 GN=mltF PE=3 SV=1
A8ZWR8	1.53e-16	162	329	289	451	Membrane-bound lytic murein transglycosylase F OS=Desulfococcus oleovorans (strain DSM 6200 / JCM 39069 / Hxd3) OX=96561 GN=mltF PE=3 SV=2
Q8EC56	1.65e-16	173	319	291	432	Membrane-bound lytic murein transglycosylase F OS=Shewanella oneidensis (strain MR-1) OX=211586 GN=mltF PE=3 SV=2
A1RHR2	2.99e-16	173	319	291	432	Membrane-bound lytic murein transglycosylase F OS=Shewanella sp. (strain W3-18-1) OX=351745 GN=mltF PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000025	0.000007	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
20	42