CAZyme Information: MGYG000003857_01432

Basic Information

• Lineage: Bacteria; Cyanobacteria; Vampirovibrionia; Gastranaerophilales; Gastranaerophilaceae; RUG841
• CAZyme ID: MGYG000003857_01432
• CAZy Family: GT8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
297		34852.03	7.6869

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003857	1817818	MAG	United States	North America

• Gene Location: Start: 2251; End: 3144 Strand: +

Full Sequence      

MAKIPVVFTFDKRIILGAAVTIKSLLDCAKPDTEYDIYVYHPDIDDKTAKEFAKMTDGTR
HSITFEYISKDKFKNAPINKGGSWTEIVYYRLLIPELLPQYDKVLYADTDVLFKGDLADV
YNTDISDYECAAVAMEPNNDKMICHKYFPENKNELTFISSFILFNSKKMREEHFVDRVFD
VIKTFNTRLKFFDLDTLNIACDKILPLPYRYGVFQSIYYNDDLNKSAEYSFLKNVYSDKD
LLNEKANTVMLHYAGDPGKPWRRKHVPEDYQKCLDTIPKSLKRYTFRDFRKKLFSKV

Enzyme Prediction     

No EC number prediction in MGYG000003857_01432.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT8	3	263	5.1e-49	0.8793774319066148

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04194	GT8_A4GalT_like	2.94e-58	4	262	1	247	A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
COG1442	RfaJ	1.61e-36	3	262	2	245	Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis].
pfam01501	Glyco_transf_8	2.54e-31	5	263	1	250	Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.
PRK15171	PRK15171	2.90e-12	3	261	25	270	lipopolysaccharide 3-alpha-galactosyltransferase.
cd00505	Glyco_transf_8	1.69e-11	38	263	34	246	Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEE26627.1	8.13e-143	1	296	1	296
ANH77125.1	2.43e-137	1	296	1	296
ALB01873.1	2.43e-137	1	296	1	296
AIT09028.1	1.19e-130	1	296	1	296
QLE78950.1	9.67e-125	4	297	5	298

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6U4B_A	2.18e-16	4	277	302	583	ChainA, WbbM protein [Klebsiella pneumoniae]
1SS9_A	1.20e-13	6	263	3	254	ChainA, alpha-1,4-galactosyl transferase [Neisseria meningitidis]
1G9R_A	1.37e-12	6	263	3	254	ChainA, GLYCOSYL TRANSFERASE [Neisseria meningitidis],1GA8_A Chain A, GALACTOSYL TRANSFERASE LGTC [Neisseria meningitidis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P27129	3.21e-13	6	263	30	274	Lipopolysaccharide 1,2-glucosyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=rfaJ PE=3 SV=2
P43974	1.97e-08	6	264	41	281	Putative glycosyltransferase HI_0258 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_0258 PE=3 SV=2
A0A0H2URH2	7.86e-07	6	261	5	229	Glycosyltransferase GlyF OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=glyF PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003857_01432.