CAZyme Information: MGYG000003858_01735

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; UMGS363
• CAZyme ID: MGYG000003858_01735
• CAZy Family: GH2
• CAZyme Description: Exo-beta-D-glucosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
823	MGYG000003858_78|CGC1	95068.36	5.3958

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003858	2536747	MAG	United States	North America

• Gene Location: Start: 6360; End: 8831 Strand: +

Full Sequence      

MMIQSLNGPWRVNSADEKIMLSVNVPGTVYSSMLENKMIEDPFYRLNQYGVLGISDKDFV
FAYDFIPDEAMLKNDRIYLRFDGIDTIAEVYLNDTAVGSADNMHRTYKFDVTDCIRRGTN
SLRVYIHSPIKYIKDKNDERDLWGVASTMKGYPHIRKAHYMFGWDWGPILPDVGIWRDVS
LVGEKYGCIKDIRIKQEHKQSSVKLYIDTEIENLTGDDLRMECVITDPDGVNTIVKTDRP
KQHNSAVCVILNPKLWHVRGFGKQYLYEVRVKLLHKGEVIDKKELKTGLRTVEVSRQKDN
IGEEFCFKVNGTKIFAMGANYIPEDNLLGRRSRERTEKLLRDCAAANYNMIRVWGGGFYP
DDYFYDICDELGLLVWQDMMFACSVYDGNDPEFCATVEQELIDNVRRIDHHACLAMWCGN
NEIESAWQYWGLPDEPDLKQGYLNMFENIAPKVIAQYDTGHFYWPSSPSSGGGFNDSGAK
NKGDIHYWEVWHSLKPFTEYKKYLFRFCSEYGFESIPCMKTVRSFAEEKDLNLMSPVMEA
HQKCEAGNEKLMYYLAQMVHYPYSFENLVYATQLVQADAIRLNVEQMRRHRGICMGSLYW
QVNDCNPVISWSSIDYFGRWKALHYYARKFYAPIICSIDDSDKDNLVLNVSNEKMTEFNG
HIKWKIRRNTTEVIAEGDSGEFTVPPLSAVNVCNITKEMTGLTLADHFNCTLEYSLMHKD
AIISSSTFLFVLPKQFEFLPPEIGVRCDTLGDKYCLTFSSQAYAKGVYIDFDEFDVVFGD
NWFDINGQDVSILLKKDVLPPYFTPELVKEKLRIKSYADIALS

Enzyme Prediction     

No EC number prediction in MGYG000003858_01735.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	693	3.7e-106	0.6888297872340425

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.14e-108	1	633	10	631	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	2.13e-27	4	422	42	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	2.70e-15	65	308	74	295	beta-D-glucuronidase; Provisional
pfam00703	Glyco_hydro_2	6.63e-12	189	290	3	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
pfam17753	Ig_mannosidase	1.83e-10	740	819	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCO04426.1	6.80e-293	5	814	6	820
AUG59052.1	6.27e-288	2	819	1	816
QNF27003.1	1.54e-275	6	821	6	820
QUH28340.1	1.66e-269	1	820	1	818
ADU28568.1	9.47e-269	5	816	5	814

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6BYE_A	4.91e-157	5	792	7	830	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]
6BYC_A	5.04e-157	5	792	7	830	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYI_A	3.61e-156	5	792	5	828	Crystalstructure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYI_B Crystal structure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYG_A	3.71e-156	5	792	7	830	Crystalstructure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYG_B Crystal structure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
2VJX_A	1.28e-152	3	785	8	806	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q29444	2.38e-104	5	783	24	844	Beta-mannosidase OS=Bos taurus OX=9913 GN=MANBA PE=1 SV=1
Q95327	4.71e-103	5	783	24	844	Beta-mannosidase OS=Capra hircus OX=9925 GN=MANBA PE=1 SV=1
A1CGA8	2.33e-102	25	658	32	680	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
Q8K2I4	4.85e-101	5	793	24	854	Beta-mannosidase OS=Mus musculus OX=10090 GN=Manba PE=1 SV=1
Q4WAH4	8.96e-101	25	658	32	680	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000058	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003858_01735.