CAZyme Information: MGYG000003863_01411

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Hydrogeniiclostridium
• CAZyme ID: MGYG000003863_01411
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
648	MGYG000003863_169|CGC1	73920.79	4.9688

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003863	2725478	MAG	United States	North America

• Gene Location: Start: 2119; End: 4065 Strand: +

Full Sequence      

MDTKTFSNPISPKDIKITDPFWARYMELARTEVIPYQWEALNDRLPDTEPSYWVKNFRVA
AGLEEGKFNGCVFQDSDGFKWLEAVAYSLMSHPDPELEKTADEVIDLICAAQQPDGYLDT
YYIINGLDKRWTNLRDHHEMYCLGHMTESAVAYYEATGKDKFLNAVIRYVDLVDANFGPE
PGKMKGYPGHEILEMALVRLYNVTKNEKHLKLAKYFIDQRGQAPLYFEEEGKKYDKPSYW
ANNKWLNNRYYQADVPVREQEFAEGHAVRAVYLYSGMASVARETGDETLLKACDRLWKDI
AERQMYITGGIGSCDVGESFTYDYDLPNDTAYNETCAAIGLMFFARRMLEIRADSSYGDV
MERALYNGVISGMSQDGKRFFYVNPLEVDPEACEKSSIHFHVEPVRQKWFGCACCPPNLA
RLLTSLGSYAYTLNDDSVYMHLFVGGDFAHTVGGKSVSFSVNTKYPWEGNIAVKMTMEGE
ASFTYALRIPGWCNGKYTLKVNGEAAACEMKNGYALLNRTWKNGDEITLELPMDVRVMTA
NLKVREDVNKVAVTCGPIVYCLEEADNGKNLHLIHLGKKRDFKVAFDPNFFGGMNVITVT
GEAQKQDDCTALYREDSEPIFEEKTLKLIPYYAWANRGVNEMTVWMYR

Enzyme Prediction     

No EC number prediction in MGYG000003863_01411.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	15	561	1.6e-232	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	0.0	9	648	2	587	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	0.0	14	561	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVK51332.1	8.89e-303	1	646	1	646
AJG99734.1	2.54e-302	1	646	1	646
AIU03254.1	1.69e-300	1	646	1	646
ABR34523.1	1.69e-300	1	646	1	646
ALB46425.1	1.69e-300	1	646	1	646

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	4.35e-248	14	646	15	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	5.76e-241	14	646	15	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	1.84e-179	14	648	2	658	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	2.62e-179	14	648	2	658	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	7.40e-179	14	648	2	658	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.01e-178	14	648	2	658	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000080	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003863_01411.