CAZyme Information: MGYG000003875_00928

Basic Information

• Species: Firm-11 sp900540045
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74; Firm-11; Firm-11 sp900540045
• CAZyme ID: MGYG000003875_00928
• CAZy Family: GT39
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1364	MGYG000003875_7|CGC3	150369.64	6.6243

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003875	3247365	MAG	United States	North America

• Gene Location: Start: 51420; End: 55514 Strand: -

Full Sequence      

MKRICAFFCLALTLLMCLTAAAEEANLIANGGFETLDASGNPEGWYPTAYRTQEGYSRIA
ITSEKAHSGQYSAVVENASANDARYTCTVSVEPSSLYRLSAYVLVDSMEDTGNGANLGIE
GIYSYSDCLFDTDGEWRYLEWYGETGEDQHEVTFGVRVGGYGAESTGKAYFDDVRLEKVD
ALPSGVFASVWYDTQSYVSYDTTTTQQTGQKSTALFVVLGLLFGFLYLLARPLLSRGGAD
RRAVYAFACVMAAALAVRIVLAVNVPGYSVDINCFTAWSLRMAERGPAGFYAADYFCDYP
PGYMLLLWPVGLLLSAAQGAGASLLVVKSLPIVCDLAGAILLFAFARKRLGDTAASLVAG
IYALNPAVLVNGAAWGQADSVLALLLMFTCLAAIRRNWRAAFPLFAAAALVKPQALLFAP
VGGVWLLGCLLEKQEQTDRARQWKSVGLGLGLAVLVAAAIIVPFSVRQSPGWLISLYQET
LSSYAYATLNTANLYYLIGANWTSLEAHVPTALPLVTALAAMSAGLWLLLDGEKLSLRAQ
PRRASLGALCLLLALYQFGLTFFGGSYTAYGYGMMAFAFLLSAVCLAFDRKANHLPFYLA
LSLILIYVLGIKVHERYLLPALPLLLTAYVLTRDRRLPGLCVGFSITTFINTAIVLDNAI
LYGAEMGHLNNDTLVLNDALCVLNLLLCGYAIWVAYTGLRPSEAPRPEKAEPSVPPAYER
MLLSPRDARLRLTLRDYLVLGATCVVYGVLAFANLGSTAAPQTGWVSTSPDEQIVFDLGE
STRFSLLYYAGVSYNDFSVSTSEDGVTWSAETPCRMREGLCYRWLYALQSTQSNGETTYL
SDSPTSVVWFTGRYLRLNACEAGLNLWEIVARDENGQTLPLTIVSHTGARTGVLESEKPV
ENLIDEQDTCVGEPGWYNGTYFDEIYHARTAYEHLHGQAPYETTHPPLGKLMMSVGIAIF
GMTPFGWRFAGALIGVLMLPALYLLALQLTHRRSIATVSMVAFSLDLMHYTQTRIATIDS
FPVFFILLSYLCMVRYLQTDLFAADPEKPRLFDRCLRRSLIPLALSGLFMGLAIASKWIG
LYAAVGLALLFFVAIYRQYRASNVACGLDMEHAKLDDSQKARVRAAQDFTLKRILITCGF
CVIFFVLVPCVIYYLCYIPYLSPTGPVTLRRVIDAQIGMLNYHSTPGLGMDHPFQSPWWQ
WPFILKPMWFAQDSYEPAGYASTIMCMGNPWVFYLGAIAMLGALVACAAKYLNLRSGVSL
RQGDGDLTLPVLAVGFLAQYLPWVLVPRSMYIYHYFASVPFIILATAWWLDRLPRSRPRL
RMGVMALYLVGALVFFVMFFPYASGWLTSTGWLDAMKWFSKLYY

Enzyme Prediction     

No EC number prediction in MGYG000003875_00928.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	918	1160	1.3e-54	0.9147982062780269

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam16192	PMT_4TMC	4.73e-23	1170	1360	1	198	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
COG1928	PMT1	3.77e-20	916	1165	43	277	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
pfam02366	PMT	1.30e-16	915	1097	16	202	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
COG1928	PMT1	1.30e-12	1169	1360	498	691	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
pfam13231	PMT_2	1.84e-10	945	1095	2	131	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUA53848.1	6.42e-311	2	1354	9	1362
QTE71236.1	9.95e-311	22	1364	11	1407
QTE75201.1	1.57e-310	22	1364	26	1422
QUC68008.1	4.39e-310	22	1364	26	1422
QTE68569.1	1.54e-308	16	1354	24	1392

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
L8F4Z2	1.92e-22	911	1345	56	498	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycolicibacterium smegmatis (strain MKD8) OX=1214915 GN=pmt PE=3 SV=1
P9WN04	1.48e-21	922	1304	73	457	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=pmt PE=3 SV=2
P9WN05	1.48e-21	922	1304	73	457	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pmt PE=1 SV=2
Q8NRZ6	6.34e-12	945	1359	99	516	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) OX=196627 GN=pmt PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000253	0.999083	0.000202	0.000156	0.000148	0.000143

TMHMM  Annotations     

start	end
213	230
243	265
305	327
354	376
415	432
445	467
508	530
543	565
569	588
595	613
617	632
639	661
674	696
737	759
965	987
1015	1037
1074	1096
1134	1156
1232	1254
1267	1286
1291	1310
1323	1345