CAZyme Information: MGYG000003891_00201

Basic Information

• Species: UBA11524 sp000437595
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74; UBA11524; UBA11524 sp000437595
• CAZyme ID: MGYG000003891_00201
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1127		124233.82	4.6926

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003891	3063528	MAG	United States	North America

• Gene Location: Start: 48523; End: 51906 Strand: +

Full Sequence      

MEQLLNDGWQFCKLPLGSTLEDVSNAALQAVDLPHDFLIENENDLYETTDAWYLRTLDVP
ESWCASCVLVRFDGVYMDCDVLLNGHVVCTHHYGYTAFDVDLTSRLLVGRNTLAVHIRHQ
SPNSRWYSGAGIYRDVTLHVLPKRHIPLDGVYVTTTHELESWTMTIDTELCGVKDNGVLT
HVLLDGDVPVCDVTVEAAGDHANAVMQVLSPKLWSPDAPNVYTLQTMLGNQVIRQNVGFR
DLTFTTDCGLLVNGAPVKLHGVCLHHDLGCLGSAFHEKAMRRQLRAMRQMGINALRTSHN
PPAKQVMDLCDELGIFVVDEAFDMWLRAKTPFDYARFFPTDMPSDVAAWVRRDRNHPSLL
MWSIGNEILDTHLDESAQQVTCDLCENVRLHDPRGNAVITIGSNFMPWEGARKCADLVDA
QGYNYGEKYYEAHHAEHPDWVIYGSETASALSSRGIYHFPMAASILSDEDLQCSALGNST
SSWGTKDMRKCIVEDLNTPYSLGQFLWSGIDYIGEPTPYHTRSCYFGMMDTAVFPKDYWY
LFKSLWTNAPMAHIGVYWDWNPGQMIDVPVMTNGVKAELLLNGRSLGVQEVSRTDWTRCR
PAWQVPFEAGELVARAYDADGHLIAEDIQRTPGESHHIVLSAEDAFLLGDGEDMTFVTIT
AADENGSPVENAVDRVLVDVDGCGRLLGLDNGDSTDRDGYKTNTRRLFSGKLLAIVGALA
GEGSIHIRVSGVGLVGAELTLPVRAARKTPGRSCSAVLCRQEEMPADKPVRRIELLPLGD
KRLGSEHPTVSFRVAVHPADADKQPIAFRVTNGQGIDSPCASCSVDGDVVTVAALGDDTV
YLRASCTNGYDHPRIISQQDIVITGLGQPFLDPYGFISGGLYSLSSGEIGNGNEQGISFA
RDGESMAGYTKIDFGDVGSDVITLPVFALDSNLYEIKLWDGDPADGGRLIAVLPYQKPSI
WNVYQSETYHLPERLTGVHTLCFSLTSKIHLKGFSFEKQSRAWLPQTAQDADTVYGDSFT
RSGSAVTGIGNNVSLVWENMDFGTSTHAELRLDGQTPLSTNPVTIRFTNQEGEQLTSLAQ
FSGTERGVQCFDVNVLPGVCSVAFVFLPGSQFDFYGFTFVKQEEAAQ

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	644	1.1e-108	0.6941489361702128

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	5.08e-72	1	520	11	565	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	2.49e-43	50	419	66	465	beta-D-glucuronidase; Provisional
PRK10340	ebgA	4.33e-41	3	367	42	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	5.72e-34	53	367	124	462	beta-galactosidase.
pfam18565	Glyco_hydro2_C5	6.97e-33	638	740	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTE68512.1	0.0	1	1121	1	1124
QTE71175.1	0.0	1	1121	1	1119
QTE75139.1	0.0	1	1121	1	1119
QUC68071.1	0.0	1	1122	1	1119
QUA53909.1	0.0	1	1122	1	1116

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	2.81e-145	6	741	7	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	1.94e-143	6	732	13	798	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5T98_A	2.55e-137	6	743	31	820	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4CU6_A	7.32e-126	6	727	19	828	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	1.98e-125	6	727	19	828	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	4.30e-124	29	741	72	846	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	5.26e-80	1	719	47	796	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	6.66e-43	5	676	56	764	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	2.40e-36	5	733	19	742	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
Q56307	2.05e-32	53	370	114	444	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999634	0.000318	0.000034	0.000003	0.000001	0.000044

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003891_00201.