CAZyme Information: MGYG000003908_01385

Basic Information

• Species: Odoribacter sp900541415
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Marinifilaceae; Odoribacter; Odoribacter sp900541415
• CAZyme ID: MGYG000003908_01385
• CAZy Family: GH101
• CAZyme Description: Endo-alpha-N-acetylgalactosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
985	MGYG000003908_6|CGC2	112470.02	6.5843

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003908	3732484	MAG	China	Asia

• Gene Location: Start: 46649; End: 49606 Strand: -

Full Sequence      

MKQIRKVILGGILLAGVYLGGGFRTGYAQSVEIFRTEKINSQEMSVVVDQDFPRVVEYRL
DKKIFRGALSAERQVVVNGKAQPCRVSFRKEGTDRVVYDFTFDDIELSFSIELVVRGQVL
EGRVSRINDDRDKLYTLAFPGQQLLSVTAEDKASAYIWCRMIPMKDAPDVYRTLQESPLD
GTARTANYLFLENGDLAAGILSNVIDERERVAFQVKQIAGRKVCQAWCPVWTYRELPTET
LEAPWFKVVIAGDGNRDGVTNWQDAALLYREEVQRPFRAEEVKTIVSSQIGFNASHLAEN
PFLRMFDNMKKCALLLDFLPQDLLVKGFQGGGHDSSFPDYAHPNVQAGGFRDLNYMIDHG
REIGLNVGLHINCTEAYPESRYFDPSILSKELGWLWGDQAVLIDKKKDLKSGKLFQRIDE
MFDSVPNIDFLYVDTYQDRGWPLIKINEKLVANHQRMYTEFDAAMDGVSTWSHGRTRLKG
KVARFIWNDCRDIFGLDPLLLGAEHHGINGWEGEAAVPRFVKEVYTRNLPTKFLQHFRLM
CWKEREAVFEGGVKMTMQDSRVRGSWGDMTFFSGLWDEQKKNFKDIKVFVPWEPLAEQPG
RIFVWDETGGKGHWVLPASWRKVKKVYVYKLTDQGRIFDRVVSVSGGAVDYTFEKETPYV
FYSRKVQEISPADMKWGDGQEIADPGFNDSSLSSWEKTGEREAVQVAHDRNGQTSLKLAA
GKVAGVSQRIGALQQNAAYYVSARVLIDGKTPRKTKLQVEALQKGKVVHAVENYLDKTTL
NGVRGWQRLRVPFEMPAGADQVRISVWAEPGTDETVVWVDDMRAYRTTPVAKDARCVLSE
DFEQRDMGWGPFVNTVKGSIYTDLSELNPGRTADVIDGNFSLKTWNQRKGLLYRTVPGLL
RLLPEQEYEIEFEYLCDRDDSYELVVKESDEPEARVLLREPVKRGKGRIRKEFSTTRYPD
AWIGIEKTNDDPVIFVMDNLKIFLR

Enzyme Prediction     

No EC number prediction in MGYG000003908_01385.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	41	551	1.4e-102	0.8033946251768034

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	2.49e-70	263	513	1	265	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	4.12e-69	276	544	1	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam18080	Gal_mutarotas_3	3.79e-39	39	262	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17451	Glyco_hyd_101C	1.05e-19	527	637	1	111	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.
pfam17974	GalBD_like	1.23e-10	838	972	24	176	Galactose-binding domain-like. Proteins containing a galactose-binding domain-like fold can be found in several different protein families, in both eukaryotes and prokaryotes. The common function of these domains is to bind to specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase, phospholipids on the outer side of the mammalian cell membrane for coagulation factor Va, membrane-anchored ephrin for the Eph family of receptor tyrosine kinases, and a complex of broken single-stranded DNA and DNA polymerase beta for XRCC1. The structure of the galactose-binding domain-like members consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AQT68159.1	1.66e-183	36	985	25	983
AQT69519.1	6.46e-158	39	928	51	930
AZN39795.1	2.48e-145	39	978	208	1143
QHW33475.1	2.59e-140	36	982	254	1207
QGY71892.1	1.51e-132	5	982	9	1024

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QEP_A	6.62e-105	36	982	9	1113	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
2ZXQ_A	8.37e-105	36	982	24	1128	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]
6QEV_B	9.38e-105	37	982	10	1113	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
6SH9_B	9.38e-105	37	982	10	1113	EngBFDARPin Fusion 4b D12 [Bifidobacterium longum subsp. longum JCM 1217]
6QFK_A	9.38e-105	37	982	10	1113	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A9WNA0	2.92e-112	39	982	52	1034	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
Q2MGH6	3.30e-75	37	926	332	1332	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	3.30e-75	37	926	332	1332	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000909	0.961542	0.036618	0.000397	0.000285	0.000210

TMHMM  Annotations     

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