CAZyme Information: MGYG000003963_00510

Basic Information

• Species: UMGS1696 sp900753285
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; CAG-272; UMGS1696; UMGS1696 sp900753285
• CAZyme ID: MGYG000003963_00510
• CAZy Family: GT5
• CAZyme Description: Glycogen synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
479		54265.96	4.7822

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003963	2551706	MAG	United Kingdom	Europe

• Gene Location: Start: 69028; End: 70467 Strand: +

Full Sequence      

MKILYVASEALPYISTGGLADVVGALPKAVKNALDEKSDIRVVIPLYPSIRDKFAEQLEL
VCEMNVKLSWRNQFCGIWKTVNNGITFYFIDNLYYFDRKMPYGNYDDGERFAFFSKCVLE
LMAAMNFYPDIMHANDWQSALAVIYLKRKYSHLDEYRDIRALFTIHNIDYQGVFDFGILS
DVFELAAWDRSIVEYNGNINLLKGAVVCADMVNTVSQTYAKEILTEYYGSGLHYILRQAD
AEGKLTGIVNGIDVDYYNPATDPDIAENYSADDMSGKAVDKAELQKLAGFEEKPETPVIA
MVSRLASHKGFDLVKCVLEEIIINNDVQFVLLGTGETELEDYFKYLAAKHPDQVCIRLEF
NKSLSKKFYAGADLFLMPSKSEPCGLSQMIASRYGTVPVVRACGGLADTIKPYNENDGSG
NGFTFDNYNAHDMMHVIDYALSLYRDHSKWADLVKNVMNVDFSWDVSAKKYIDIYKKML

Enzyme Prediction     

EC	2.4.1.21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT5	2	477	5.3e-173	0.9978813559322034

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0297	GlgA	0.0	1	479	1	479	Glycogen synthase [Carbohydrate transport and metabolism].
TIGR02095	glgA	0.0	1	477	1	472	glycogen/starch synthase, ADP-glucose type. This family consists of glycogen (or starch) synthases that use ADP-glucose (EC 2.4.1.21), rather than UDP-glucose (EC 2.4.1.11) as in animals, as the glucose donor. This enzyme is found in bacteria and plants. Whether the name given is glycogen synthase or starch synthase depends on context, and therefore on substrate. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
PRK00654	glgA	0.0	1	479	1	464	glycogen synthase GlgA.
cd03791	GT5_Glycogen_synthase_DULL1-like	0.0	2	477	1	474	Glycogen synthase GlgA and similar proteins. This family is most closely related to the GT5 family of glycosyltransferases. Glycogen synthase (EC:2.4.1.21) catalyzes the formation and elongation of the alpha-1,4-glucose backbone using ADP-glucose, the second and key step of glycogen biosynthesis. This family includes starch synthases of plants, such as DULL1 in Zea mays and glycogen synthases of various organisms.
PRK14099	PRK14099	7.15e-105	1	479	4	480	glycogen synthase GlgA.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AFS77673.1	9.30e-165	1	478	1	475
AGX05960.1	2.46e-162	1	479	1	475
AIQ18725.1	1.23e-161	1	479	1	473
BCI60006.1	1.80e-161	1	478	1	472
ARP51332.1	2.83e-160	1	479	1	472

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3D1J_A	6.58e-90	1	478	1	476	ChainA, Glycogen synthase [Escherichia coli]
2QZS_A	1.16e-89	1	478	1	476	CrystalStructure of Wild-type E.coli GS in complex with ADP and Glucose(wtGSb) [Escherichia coli],2R4T_A Crystal Structure of Wild-type E.coli GS in Complex with ADP and Glucose(wtGSc) [Escherichia coli],2R4U_A Crystal Structure of Wild-type E.coli GS in complex with ADP and Glucose(wtGSd) [Escherichia coli],3GUH_A Crystal Structure of Wild-type E.coli GS in complex with ADP and DGM [Escherichia coli K-12]
3COP_A	9.07e-89	1	478	1	476	ChainA, Glycogen synthase [Escherichia coli],3CX4_A Chain A, Glycogen synthase [Escherichia coli]
4HLN_A	7.82e-76	2	476	126	623	Structureof barley starch synthase I in complex with maltooligosaccharide [Hordeum vulgare]
6GNE_A	2.52e-75	1	477	10	496	Catalyticdomain of Starch Synthase IV from Arabidopsis thaliana bound to ADP and acarbose [Arabidopsis thaliana],6GNE_B Catalytic domain of Starch Synthase IV from Arabidopsis thaliana bound to ADP and acarbose [Arabidopsis thaliana]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q72YJ6	1.17e-150	1	479	1	475	Glycogen synthase OS=Bacillus cereus (strain ATCC 10987 / NRS 248) OX=222523 GN=glgA PE=3 SV=1
A4J4I4	1.90e-150	1	479	1	475	Glycogen synthase OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=glgA PE=3 SV=1
Q816G8	2.35e-150	1	479	1	475	Glycogen synthase OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711) OX=226900 GN=glgA PE=3 SV=1
B7HBC4	2.35e-150	1	479	1	475	Glycogen synthase OS=Bacillus cereus (strain B4264) OX=405532 GN=glgA PE=3 SV=1
Q81K85	3.33e-150	1	479	1	475	Glycogen synthase OS=Bacillus anthracis OX=1392 GN=glgA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000047	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003963_00510.