CAZyme Information: MGYG000003993_00806

Basic Information

• Species: Alistipes sp900550925
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; Alistipes sp900550925
• CAZyme ID: MGYG000003993_00806
• CAZy Family: GH18
• CAZyme Description: Chitinase A1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
341		38665.07	4.431

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003993	1895769	MAG	United Kingdom	Europe

• Gene Location: Start: 15716; End: 16741 Strand: -

Full Sequence      

MSGERVDGRVVVAYVTYYGKATPDPDYFTNINYGFAELYMKNGVYEGFKLQGDESRFREI
VNLKRSHPELKISLSFTHTVSNSDNSQGGGFSDLAKSDAYRKAFAEDCRTFCASWGIDGI
DIDWEFPGLSWSGHKCDPEVDVENFTLLMKQLRETLGTRYLLTYAGYVKDVQDIEGGGRK
YIDVAAVDPYVDYVNIMTYDIASAPQHQSALRDPDCYWDCERAVNAYIEAGVSTSKLVLG
IPFYGRHSFDVSPTAIDYKDIIQLDKKDYKIDNWDEEASVPYVTYKGNYYCGYDNPKSIA
IKAQWALDLGMKGLMYWDYDGDDNAGTLRTAVWQETMKADE

Enzyme Prediction     

No EC number prediction in MGYG000003993_00806.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	11	326	1.4e-60	0.9425675675675675

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06548	GH18_chitinase	4.03e-79	11	322	1	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
smart00636	Glyco_18	1.08e-64	10	322	1	334	Glyco_18 domain.
pfam00704	Glyco_hydro_18	4.60e-63	10	322	1	307	Glycosyl hydrolases family 18.
cd02872	GH18_chitolectin_chitotriosidase	2.22e-57	11	327	1	346	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.
COG3325	ChiA	2.51e-39	4	332	33	432	Chitinase, GH18 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGY54522.1	2.97e-173	4	338	41	375
QEC44030.1	8.74e-155	4	338	37	370
QPH57581.1	1.56e-151	4	338	41	374
QMI82040.1	1.27e-150	4	338	41	374
QQA31286.1	1.80e-150	4	338	41	374

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3QOK_A	2.06e-33	29	323	55	404	ChainA, Putative chitinase II [Klebsiella pneumoniae subsp. pneumoniae MGH 78578]
6TSB_AAA	6.17e-33	59	317	80	332	ChainAAA, Peroxiredoxin [Clostridioides difficile 630]
6INX_A	7.98e-33	29	334	28	340	Structuralinsights into a novel glycoside hydrolase family 18 N-acetylglucosaminidase from Paenibacillus barengoltzii [Paenibacillus barengoltzii]
6T9M_AAA	8.54e-33	59	317	99	351	ChainAAA, Peroxiredoxin [Clostridioides difficile 630]
4NZC_A	2.76e-32	29	324	35	385	Crystalstructure of Chitinase D from Serratia proteamaculans at 1.45 Angstrom resolution [Serratia proteamaculans 568],4Q22_A Crystal structure of Chitinase D from Serratia proteamaculans in complex with N-acetyl glucosamine at 1.93 Angstrom resolution [Serratia proteamaculans 568]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20533	2.31e-27	13	340	55	450	Chitinase A1 OS=Niallia circulans OX=1397 GN=chiA1 PE=1 SV=1
Q95M17	4.04e-27	24	328	47	371	Acidic mammalian chitinase OS=Bos taurus OX=9913 GN=CHIA PE=1 SV=1
P11220	7.38e-26	55	332	318	605	Chitinase 63 OS=Streptomyces plicatus OX=1922 GN=chtA PE=1 SV=2
Q9W092	7.48e-26	9	329	41	396	Probable chitinase 2 OS=Drosophila melanogaster OX=7227 GN=Cht2 PE=1 SV=1
P36362	2.07e-25	49	326	75	379	Endochitinase OS=Manduca sexta OX=7130 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000061	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003993_00806.