CAZyme Information: MGYG000004003_01491

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides
• CAZyme ID: MGYG000004003_01491
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
762	MGYG000004003_20|CGC2	85999.03	6.907

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004003	5653807	MAG	United Kingdom	Europe

• Gene Location: Start: 22705; End: 24993 Strand: -

Full Sequence      

MTIGITLLLIGYIFCLPRQLFHVPYSTVVTDRNEELLGARIASDGQWRFPPRETTPEKIK
QCLITFEDKHFYHHWGVNPLSIGRAIYQNTRHKRIVSGGSTLTMQTIRLARNESRTFGEK
VIEMIWATRLEFRASKEEILSMYVSHAPFGGNVVGLDAAAWRYFGHSAEDLSWAESAMLA
VLPNAPAMIHLSKGRQMLLSKRNRLLKQLLEKKIIDSSTYELAVTEPLPDEPHPLPQTAP
HLVSRFYKERNGQYTRSTIDRGIQTHIEDLAERWSNEFNRSDIRNLAILVIDIPANQVAA
YCGNVHFDRKQGGNQVDVIQAPRSTGSILKPFLYYAMLQEGSLLPDMLLPDIPININGYT
PQNFSMQYEGAVPASEALARSLNIPAVTMLQKYGVPKFHNFLQQTGFRTITRPSSHYGLS
LILGGAEATLWDVTNAYAQMGRALTPNLSPELTKEKEAGIIIKEEENKTVQHEYIWDKGA
VWQTFNALKEVNRPEEIDWKSIPSMQTIAWKTGTSYGFRDAWAVGVTPRYAVGVWVGNAT
GEGKPGLVGAQTAGPVLFDIFSYLPASPWFERPTDVFVDAEVCRQSGHLKGRFCEETDTV
LILPAGLRTDACPYHHLITLAADEKHRVYESCANTEPTLQKSWFTLPPVWEWYYKQHHPE
YNPLPPFKAGCGEDTFQPMQFIYPPMNAHIKLPKQLDGSKGFLTVELAHTNPNANVFWHL
DDTYLMQTQDFHKISLQPAPGKHSLTAVDGEGNTVSTTFFIE

Enzyme Prediction     

No EC number prediction in MGYG000004003_01491.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	51	209	1.2e-45	0.8983050847457628

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02073	PBP_1c	0.0	17	762	2	727	penicillin-binding protein 1C. This subfamily of the penicillin binding proteins includes the member from E. coli designated penicillin-binding protein 1C. Members have both transglycosylase and transpeptidase domains and are involved in forming cross-links in the late stages of peptidoglycan biosynthesis. All members of this subfamily are presumed to have the same basic function. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG4953	PbpC	0.0	7	762	17	732	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
PRK11240	PRK11240	7.80e-126	43	557	57	555	penicillin-binding protein 1C; Provisional
COG0744	MrcB	8.73e-105	23	564	52	596	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	2.52e-99	56	563	11	527	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIU95316.1	0.0	5	762	22	788
QUU08526.1	0.0	5	762	29	791
QRP59812.1	0.0	5	762	23	785
ASM66196.1	0.0	5	762	23	785
QQT77232.1	0.0	5	762	23	785

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FGZ_A	5.76e-33	49	538	158	668	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	6.20e-33	49	538	179	689	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]
3FWL_A	1.69e-30	49	538	162	672	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli]
2OQO_A	1.93e-28	56	226	29	200	Crystalstructure of a peptidoglycan glycosyltransferase from a class A PBP: insight into bacterial cell wall synthesis [Aquifex aeolicus VF5],3D3H_A Crystal structure of a complex of the peptidoglycan glycosyltransferase domain from Aquifex aeolicus and neryl moenomycin A [Aquifex aeolicus],3NB7_A Crystal structure of Aquifex Aeolicus Peptidoglycan Glycosyltransferase in complex with Decarboxylated Neryl Moenomycin [Aquifex aeolicus]
4OON_A	2.98e-28	38	438	28	547	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P76577	2.33e-98	17	564	33	564	Penicillin-binding protein 1C OS=Escherichia coli (strain K12) OX=83333 GN=pbpC PE=1 SV=1
Q9KUC0	4.90e-40	43	571	176	725	Penicillin-binding protein 1B OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mrcB PE=3 SV=1
P40750	1.16e-39	31	540	60	599	Penicillin-binding protein 4 OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpD PE=1 SV=2
P38050	8.25e-39	5	537	28	569	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
P45345	1.22e-38	23	553	143	693	Penicillin-binding protein 1B OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=mrcB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.985122	0.014630	0.000184	0.000024	0.000017	0.000038

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004003_01491.