CAZyme Information: MGYG000004017_00109

Basic Information

• Species: HGM11588 sp900754755
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74; HGM11588; HGM11588 sp900754755
• CAZyme ID: MGYG000004017_00109
• CAZy Family: GH2
• CAZyme Description: Exo-beta-D-glucosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
835	MGYG000004017_1|CGC2	95905.11	5.7491

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004017	3620670	MAG	United Kingdom	Europe

• Gene Location: Start: 124333; End: 126840 Strand: +

Full Sequence      

MEQIFLQEGWRLREEPLSMQRESVGAVLAQEQGWMDCSLPCDVSMALMAHGRIADPVVAM
HCYENEWIERRSWWFVKEFSWAEAARYDAVELVFPSLDCHADVFLNGAYLGHQASAHYPF
RREVSRHLRSGVNRIVVRLTVGLEYVSDGDLAQIDRAVCTERGNGRTDRSDKRRAFLRKP
QYVFGWDWSPRIATCGIMEVAYLELHRKVAVRALHIATQKLADGQAWLRGEIVVENLDML
GTLDADARLSLCLKGETICAIEWRDQWLASGIQHLPFEARIPHPRLWWPNGYGEQPLYEA
KCTVSVAGEVLAERNESFGIRTVELDTSRDETGWRRFAFRINGREIFCKGANWIPADAIY
ARVDDARFATLLREAKEANFNMLRVWGGGLYNREIFYRLCDELGILLWHDFMFGCACYPD
HLEAFRLECEREMEFQTRRLRNHPCMALFCGNNENHQIFDWQANPGWGIHPGLDKQYGLL
LANRCAREAVWRNCPEIPYWNSSPYGGARPGEEDEGDVHYWGQCMMNPDRQKRIEPREYE
RVHARFVSEYGYPGPTCLATMREYLGQEAIDRNSEAWKLHTNTFEKDTVAAGIAKHYGEP
EGLSLEEYILYAGMVQSTMLEESLETLRAKLFCAGSLFWMYNDCWGEVGWSIVDYALRRK
IAYYGVKRAFSPIRLILRQEKENVIAVGCNDAPEPVFLEGMLGYMAFDGSSAQQRHVRLE
IPAGRSVVLREALPQYDVRRGVFAFLPDAPEADAAVLRRTDIRQMEIAGQAQIEECQAVG
EDLAVVVRASRYTHGVYFAGDMPCSEEYFDLLPGQRKRVILYGCAGQTPVLRWVR

Enzyme Prediction     

No EC number prediction in MGYG000004017_00109.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	6	685	4.3e-83	0.6236702127659575

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	2.58e-61	1	677	10	636	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam00703	Glyco_hydro_2	6.88e-08	209	321	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10150	PRK10150	1.79e-07	279	454	237	417	beta-D-glucuronidase; Provisional
PRK10340	ebgA	4.19e-06	86	405	131	392	cryptic beta-D-galactosidase subunit alpha; Reviewed
PHA03231	PHA03231	0.005	161	195	253	288	glycoprotein BALF4; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBE97186.1	2.12e-285	1	835	1	836
QMW77011.1	6.84e-282	1	823	1	826
QBE99829.1	6.84e-282	1	823	1	826
QIB55134.1	6.84e-282	1	823	1	826
BBI35730.1	2.49e-252	4	822	3	825

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	2.09e-123	30	731	39	714	Crystalstructure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	3.01e-102	34	821	25	816	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	3.15e-102	34	821	27	818	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	3.21e-102	34	821	27	818	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	8.32e-102	34	821	25	816	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q5B7W2	4.76e-81	38	680	31	663	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
Q0CCA0	6.29e-80	32	833	24	838	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
Q4WAH4	4.49e-79	1	680	4	663	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
A2QYN2	6.10e-79	32	680	24	663	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1
B0YBU9	8.55e-79	1	680	4	663	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000038	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004017_00109.