CAZyme Information: MGYG000004039_01582

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UMGS2037
• CAZyme ID: MGYG000004039_01582
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
370	MGYG000004039_14|CGC1	41798.64	4.2674

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004039	2803182	MAG	United Kingdom	Europe

• Gene Location: Start: 11115; End: 12227 Strand: +

Full Sequence      

MRLPDDFDDEENNSMGLPMIHMVIGVAAFILVLFAIVIAVNYKNTGHHNKNTDTAIPVET
SENQETKDLKERTEELISGSTLRSDDLDFWDMYPEQETKETQVKETEETKEEPKDPSTDG
KHTLVTYASGRQEWVSINPYIKKNSYDYTNLVSQNNIMKYYEGSRLTSFLGVDISKNDGT
VDFDMLKAAGVSFVMIRTGSRGYESGQLMEDELFTEHMDGALAAGLDVGLYFSSAAISKE
EAEEEANMLLAQIGERKITYPIAFQMAFADNDVSRFDKLTAAQRTEITRAFLNVISNAGY
RPMVYGDKEWLIKEIDLSKLGDYDFWLSQPGDLPDYPYAFSIWQYSTTKNIDGINGTAHV
NISFIDYASK

Enzyme Prediction     

No EC number prediction in MGYG000004039_01582.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	172	354	1.7e-34	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06414	GH25_LytC-like	4.38e-62	171	364	3	191	The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves the beta1-4-glycosydic bond located between the N-acetylmuramoyl-N-glucosaminyl residues of the cell wall polysaccharide chains. LytC is composed of a C-terminal glycosyl hydrolase family 25 (GH25) domain and an N-terminal choline-binding module (CBM) consisting of eleven homologous repeats that specifically recognizes the choline residues of pneumococcal lipoteichoic and teichoic acids. This domain arrangement is the reverse of the major pneumococcal autolysin, LytA, and the CPL-1-like lytic enzymes of the pneumococcal bacteriophages, in which the CBM (consisting of six repeats) is at the C-terminus. This model represents the C-terminal catalytic domain of the LytC-like enzymes.
cd00599	GH25_muramidase	2.30e-23	171	362	2	185	Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
pfam01183	Glyco_hydro_25	6.01e-21	172	354	1	180	Glycosyl hydrolases family 25.
cd06525	GH25_Lyc-like	2.92e-17	171	361	2	182	Lyc muramidase is an autolytic lysozyme (autolysin) from Clostridium acetobutylicum encoded by the lyc gene. Lyc has a glycosyl hydrolase family 25 (GH25) catalytic domain. Endo-N-acetylmuramidases are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
COG3757	Acm	2.17e-16	135	361	36	251	Lyzozyme M1 (1,4-beta-N-acetylmuramidase), GH25 family [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM01196.1	5.93e-114	1	370	1	373
QHI72152.1	2.44e-55	144	367	68	291
QEK18217.1	2.32e-54	145	364	77	298
QRT51228.1	3.10e-54	117	365	36	285
QCT06482.1	2.29e-52	118	367	68	319

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P34020	1.39e-07	171	361	3	178	Autolytic lysozyme OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=lyc PE=1 SV=1
P26836	2.66e-06	162	361	2	194	Probable autolytic lysozyme OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=lyc PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000059	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
20	42