CAZyme Information: MGYG000004053_00766

Basic Information

• Lineage: Bacteria; Verrucomicrobiota; Verrucomicrobiae; Opitutales; CAG-312; CAG-312
• CAZyme ID: MGYG000004053_00766
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
732		83386.3	5.7897

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004053	2223892	MAG	United Kingdom	Europe

• Gene Location: Start: 20959; End: 23157 Strand: +

Full Sequence      

MIISKDEIEILTSARCGQPHDLLGMHKCRGGIVVRAYLRDAKTCKLIDLRDEKRTLPPMK
RLHESGLFETFIKGARKPFPYAFKVRSYNGDTRKIADPYAFPPTLSDDDLYLIGKGDDKK
IYLKLGSHMRTIDGVSGTSFAVWAPTARRVSVVGDFNDWDGRYHQMRELGQSGIWEIFVP
AVGDGAKYKFEIVAARNETPFLKIDPYALRFEAPPYNSSIVCDISGFEWSDEKWLADRAQ
TDWSKRPVSVYEVHLGSWKRVPEDGFRPLAYAEIGTQLAEYCVEMGFTHVEFLPLSEYPF
EGSWGYQVTGYYAPTHRYGSPLDFMKMVDTLHKHGIGVIMDWVPAHFPRDSFALAGYDGS
CLYEHQDPRLGSNPDWGTLCFNYGRSEVSNFLMGSALAWFDRFHIDGLRVDAVASMLYLN
FSRRDWLPNRYGGSENLEAIEFIKRTNTAVHEEFKGSIMIAEESTTFRGVTAPVADGGLG
FDFKWNLGWMHDTLDYFKEDPINRKYHHNKMTFPSMYQFTEKFMLVYSHDEVVHGKSPMV
GKMGGAYWGDKIANLRALYVYMWMWPGKKTLFMGDEIAQGHEWRYDESLEWSLLQYAEHE
GVKDVVRDIATLYKTDTSLAENDFNPQTFEWINADDGDNSVFTFLRFGADGKSAYAVACN
FTPIERDSYAIGLPLGGRWDEVLNTDAKCYGGGGRGNNGYLLAEKADVNNRPFAVFATLP
PMSAIVFKHEEE

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	280	579	1.2e-143	0.9966777408637874
CBM48	124	212	2.3e-20	0.9210526315789473

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK05402	PRK05402	0.0	3	730	1	724	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	0.0	3	728	503	1221	glycogen branching enzyme; Provisional
PRK12313	PRK12313	0.0	103	730	5	628	1,4-alpha-glucan branching protein GlgB.
PRK14706	PRK14706	0.0	125	729	27	621	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	100	728	1	627	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QYY37490.1	8.51e-317	27	729	7	709
QXD26686.1	2.80e-314	1	732	1	738
QXD22608.1	2.80e-314	1	732	1	738
ADE53954.1	2.39e-311	25	732	1	713
AKC82531.1	9.81e-305	1	731	1	735

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQZ_A	6.14e-248	3	728	25	772	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQW_A	8.69e-248	3	728	25	772	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	2.47e-247	3	728	25	772	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	4.95e-247	3	728	25	772	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR1_A	9.93e-247	3	728	25	772	Crystalstructure of branching enzyme Y500A/D501A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142],5GR6_A Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2W2Q6	1.02e-263	2	730	17	739	1,4-alpha-glucan branching enzyme GlgB OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=glgB PE=3 SV=1
Q165E2	7.95e-261	1	731	1	730	1,4-alpha-glucan branching enzyme GlgB OS=Roseobacter denitrificans (strain ATCC 33942 / OCh 114) OX=375451 GN=glgB PE=3 SV=1
Q1D654	1.25e-260	7	727	13	731	1,4-alpha-glucan branching enzyme GlgB OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=glgB PE=3 SV=1
B3PGN4	8.44e-257	3	728	10	735	1,4-alpha-glucan branching enzyme GlgB OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=glgB PE=3 SV=1
Q11EX1	4.04e-255	8	730	18	737	1,4-alpha-glucan branching enzyme GlgB OS=Chelativorans sp. (strain BNC1) OX=266779 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000073	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004053_00766.