CAZyme Information: MGYG000004081_02095

Basic Information

• Species: UCG-010 sp003150215
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; CAG-382; UCG-010; UCG-010 sp003150215
• CAZyme ID: MGYG000004081_02095
• CAZy Family: GH25
• CAZyme Description: Lysozyme M1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
250		27744.52	4.8204

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004081	2523774	MAG	United Kingdom	Europe

• Gene Location: Start: 13081; End: 13833 Strand: +

Full Sequence      

MIGERGLVVKARFRFCLLLSAAALAAVAALVFFGVLPLNAPPEDRYPVRGVDVSAHQGVI
DWPMLAAQGVEFAFIKATEGSGFTDARFSFNVLQARNAGLRVGAYHFFSFESSGDAQADN
FIAVVEPFAGMLPPVVDLEYYAGFFDDPPAAEPVRAELDALLERLRAHYGVQPILYVTEA
SYARYIAGVPAYAGLDVWIRSVYRRPRLEGWTFWQYSNRARLDGYDGDERYIDLNVFCGS
RAEFEAKYPA

Enzyme Prediction     

No EC number prediction in MGYG000004081_02095.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	51	224	1.2e-46	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06413	GH25_muramidase_1	5.85e-88	46	239	1	191	Uncharacterized bacterial muramidase containing a glycosyl hydrolase family 25 (GH25) catalytic domain. Endo-N-acetylmuramidases are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
cd00599	GH25_muramidase	1.16e-54	49	237	1	186	Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
COG3757	Acm	3.46e-47	32	240	44	256	Lyzozyme M1 (1,4-beta-N-acetylmuramidase), GH25 family [Cell wall/membrane/envelope biogenesis].
cd06524	GH25_YegX-like	1.10e-43	49	239	1	194	YegX is an uncharacterized bacterial protein with a glycosyl hydrolase family 25 (GH25) catalytic domain that is similar in sequence to the CH-type (Chalaropsis-type) lysozymes of the GH25 family of endolysins.
pfam01183	Glyco_hydro_25	3.04e-42	51	224	1	179	Glycosyl hydrolases family 25.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANU41587.1	2.56e-79	17	245	16	244
QQR05545.1	2.56e-79	17	245	16	244
QIA31658.1	1.03e-78	17	245	16	244
BCI60136.1	7.48e-77	19	244	15	241
QVI64037.1	2.78e-74	28	246	8	226

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2WAG_A	1.36e-71	44	245	12	214	TheStructure of a family 25 Glycosyl hydrolase from Bacillus anthracis. [Bacillus anthracis str. Ames]
1JFX_A	3.72e-23	44	246	1	211	Crystalstructure of the bacterial lysozyme from Streptomyces coelicolor at 1.65 A resolution [Streptomyces coelicolor]
4KRU_A	2.81e-09	48	237	20	207	X-raystructure of catalytic domain of endolysin from clostridium perfringens phage phiSM101 [Clostridium phage phiSM101]
6ZM8_A	4.15e-09	48	246	2	203	ChainA, muramidase [Sodiomyces alcalophilus]
4KRT_A	5.56e-09	48	237	20	207	X-raystructure of endolysin from clostridium perfringens phage phiSM101 [Clostridium phage phiSM101],4KRT_B X-ray structure of endolysin from clostridium perfringens phage phiSM101 [Clostridium phage phiSM101]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P76421	7.30e-27	46	246	65	263	Uncharacterized protein YegX OS=Escherichia coli (strain K12) OX=83333 GN=yegX PE=3 SV=2
Q8X7H0	1.02e-26	46	246	65	263	Uncharacterized protein YegX OS=Escherichia coli O157:H7 OX=83334 GN=yegX PE=3 SV=2
Q8FFY2	1.99e-26	46	245	65	262	Uncharacterized protein YegX OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=yegX PE=3 SV=2
P25310	8.65e-23	42	246	76	288	Lysozyme M1 OS=Streptomyces globisporus OX=1908 GN=acm PE=1 SV=1
P34020	1.02e-09	48	237	1	180	Autolytic lysozyme OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=lyc PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000031	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
13	35