CAZyme Information: MGYG000004114_00646

Basic Information

• Species: CAG-95 sp000438155
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-95; CAG-95 sp000438155
• CAZyme ID: MGYG000004114_00646
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
625	MGYG000004114_5|CGC1	71617.97	4.8103

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004114	2992180	MAG	United Kingdom	Europe

• Gene Location: Start: 35924; End: 37801 Strand: +

Full Sequence      

MKHSQMQIEDCFWSPVISKMCNEIIPYQYEVLNDEIEGIPSSHAIENFRIASGQKNGKYV
GMLFQDSDVAKWIEAAAYSLKVNDDPEIRKKIEDVIDLIEQTQQEDGYLDTYYICEKPDQ
RLKNIAHGHEMYCAGHLLEAAVGYAEVTGEERFLHIMERYIKWFMDRIGPDENQLHIYPG
HPELELALYKLYQYTGNDQYLEFMKYILMERGKQPSFLLEDSGYGEQYHDKWFALEYHQA
HKPVLEQTEAVGHAVRAMYLYSGMADLAKASGDEALFNALKVLWTDVTTKKMYITGAIGS
DEHGEGFSIAYDLPNDRAYAETCASIGLFMWARRMLKLEWNSNYADVMEVALYNGISSGM
SEDGKQYFYVNPLEVNPAKVHQRYDMRHVRSSRVTWFGCACCPPNVARILTSLERYSYDI
ADDFCSMAIHLYLCGRIIMPDDSVWNITGSYLTDGKIEVSYTGEEKDFTLKLRNPGWSTG
TEIRMNGQPVEAEQEQGYFSVSRKWKDGDKLQLKFGITAQLVYANTCVEDDAGKVAVMRG
PVVFCLEEADNGKHLSELMIESIDSQCQDSVILKGYREHTEDHKALYSTIPPRIEETSIK
AIPYSHWGNAGEGEMRVWVRKVSTF

Enzyme Prediction     

No EC number prediction in MGYG000004114_00646.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	7	545	4.1e-196	0.9961832061068703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	0.0	24	620	30	586	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	6.78e-152	10	545	6	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG4225	YesR	1.89e-04	127	202	38	102	Rhamnogalacturonyl hydrolase YesR [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGH34297.1	1.80e-210	1	621	10	640
AQS56927.1	8.70e-199	6	621	12	643
ADN02408.1	1.19e-198	23	621	31	642
QSO49282.1	9.59e-197	1	620	7	637
ADU94272.1	2.27e-195	8	621	10	639

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	1.23e-195	4	621	14	647	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	1.23e-187	4	621	14	647	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	1.72e-144	12	621	9	658	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	2.39e-144	12	621	9	658	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	6.71e-144	12	621	9	658	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	9.40e-144	12	621	9	658	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000058	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004114_00646.