CAZyme Information: MGYG000004132_01986

Basic Information

• Species: V9D3004 sp900760345
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; V9D3004; V9D3004 sp900760345
• CAZyme ID: MGYG000004132_01986
• CAZy Family: GH43
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
854	MGYG000004132_37|CGC1	94213.54	6.3872

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004132	2510246	MAG	United Kingdom	Europe

• Gene Location: Start: 7244; End: 9808 Strand: -

Full Sequence      

MMIKKIKKVMICIIIAALTMTQTPQIAKARSSSSSDNGDGTFTNPNVYLDVPDIDMIRVG
ENYYMVSTTMHMSPGCPILKSTDLVNWETVNYVFDRLTDSDAANLVNGKSMYGAGQWAAS
IKYHNNMYYVAFNSNTTGTAYIFSTDDIENGSWERVELGKSYHDCALFWDDANDGACYLV
YGNNTIQYVELNDDLSGVKSGGRSGVMFEGNIDENGKDLFPGAGGLGNEGSHVYYKDGYY
YVFTINAHPNVRTEVVHRSSVFPGTNADWQTRIVLKTRFDNFGINNLGNGVAQGGIIDTT
SGEWYCYMFQDHDGIGRVPVLTKLTWTKDGWPAAGENGDTKTVANIMDIPGIPSGKKTLV
KSDEFYNDAVHKQYEAVQAVNTEEADYNGSNLDMCWEWNHNPDNTKWSLTEREGYLRLYT
AGTAKGWLNARNTLTQRTYGPTSSYAISMDVSNMNSGDIAGLGGLANKYGYVGVRCNDDG
TKEIIMVDGSDNSGANNADAAVSEAVACENDTVYLKADFLFANTEKEYDQMDFYYSYNGK
TWNKIGTSAHTKYDIVPMFMGIRVGIFNYATTNEGGYVDVDYFHVTSELTGENEYMGDAS
AELKVSADNDTISLYMDNITSSDYAGIKASIVLPKELNVRSVDYNTAAIKNGEGSYTYEN
GRLILNVKGKNVTFDAANRLFATIHVKSNTSVTSDKAGKIKTDYIYVDDGYIDYNVSNCN
PDIIIKTYVPDPTTQTDNPPVQSVTAPPDNSSSASPFVTKVTLAKVKGISAKKKGLKKAV
VKWKKVSGAEKYEVIYSADKKMKKAVKKLSVKKTTVTIKKLKNKKTYYVKVRACAKNSDG
SMSYGKYSAKKKVK

Enzyme Prediction     

No EC number prediction in MGYG000004132_01986.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH43	43	332	4.3e-89	0.996309963099631

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd09001	GH43_FsAxh1-like	7.77e-121	41	332	1	267	Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase. This glycosyl hydrolase family 43 (GH43) includes mostly enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase; xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. This subfamily includes the characterized Clostridium stercorarium F-9 beta-xylosidase Xyl43B. It also includes Humicola insolens AXHd3 (HiAXHd3), a GH43 arabinofuranosidase (EC 3.2.1.55) that hydrolyzes O3-linked arabinose of doubly substituted xylans, a feature of the polysaccharide that is recalcitrant to degradation. It possesses an additional C-terminal beta-sandwich domain such that the interface between the domains comprises a xylan binding cleft that houses the active site pocket. The HiAXHd3 active site is tuned to hydrolyze arabinofuranosyl or xylosyl linkages, and the topology of the distal regions of the substrate binding surface confers specificity. It also includes Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase (Axh1;Fisuc_1769;FSU_2269), Paenibacillus sp. E18 alpha-L-arabinofuranosidase (Abf43A), Bifidobacterium adolescentis ATCC 15703 double substituted xylan alpha-1,3-L-specific arabinofuranosidase d3 (AXHd3;AXH-d3;BaAXH-d3;BAD_0301;E-AFAM2), and Chrysosporium lucknowense C1 arabinoxylan hydrolase / double substituted xylan alpha-1,3-L-arabinofuranosidase (Abn7;AXHd). A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
COG3507	XynB2	2.57e-55	36	583	15	544	Beta-xylosidase [Carbohydrate transport and metabolism].
pfam04616	Glyco_hydro_43	1.92e-43	42	332	1	281	Glycosyl hydrolases family 43. The glycosyl hydrolase family 43 contains members that are arabinanases. Arabinanases hydrolyze the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The structure of arabinanase Arb43A from Cellvibrio japonicus reveals a five-bladed beta-propeller fold. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd08989	GH43_XYL-like	1.97e-41	44	327	1	272	Glycosyl hydrolase family 43, beta-D-xylosidases and arabinofuranosidases. This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity, including Selenomonas ruminantium beta-D-xylosidase SXA. These are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. It also includes various GH43 family GH43 arabinofuranosidases (EC 3.2.1.55) including Humicola insolens alpha-L-arabinofuranosidase AXHd3, Bacteroides ovatus alpha-L-arabinofuranosidase (BoGH43, XynB), and the bifunctional Phanerochaete chrysosporium xylosidase/arabinofuranosidase (Xyl;PcXyl). GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
pfam17851	GH43_C2	8.71e-37	386	585	3	202	Beta xylosidase C-terminal Concanavalin A-like domain. This domain is found to the C-terminus of the pfam04616 domain. This domain adopts a concanavalin A-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBK97000.1	1.27e-159	40	589	79	600
CBL35181.1	4.24e-157	39	589	66	588
QDO67769.1	3.73e-146	44	588	28	518
QOG00760.1	6.48e-143	44	585	26	514
ALJ59291.1	1.57e-141	44	586	28	516

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6MLY_A	7.04e-127	43	590	22	515	ChainA, Bifunctional GH43-CE protein [Bacteroides eggerthii],6MLY_B Chain B, Bifunctional GH43-CE protein [Bacteroides eggerthii],6MLY_C Chain C, Bifunctional GH43-CE protein [Bacteroides eggerthii],6MLY_D Chain D, Bifunctional GH43-CE protein [Bacteroides eggerthii]
3ZXK_A	2.85e-48	41	589	7	535	Engineeringthe active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity [Humicola insolens],3ZXK_B Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity [Humicola insolens]
3ZXL_A	5.26e-48	41	589	7	535	Engineeringthe active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity [Humicola insolens],3ZXL_B Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity [Humicola insolens]
3ZXJ_A	1.32e-47	41	583	7	529	Engineeringthe active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity [Humicola insolens],3ZXJ_B Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity [Humicola insolens]
6MS3_A	1.04e-32	41	583	29	533	Crystalstructure of the GH43 protein BlXynB mutant (K247S) from Bacillus licheniformis [Bacillus licheniformis DSM 13 = ATCC 14580],6MS3_B Crystal structure of the GH43 protein BlXynB mutant (K247S) from Bacillus licheniformis [Bacillus licheniformis DSM 13 = ATCC 14580]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KN85	3.61e-51	21	583	30	580	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22160 PE=1 SV=1
Q9WXE8	3.37e-32	39	451	38	394	Putative beta-xylosidase OS=Prevotella ruminicola OX=839 PE=3 SV=2
A7LXT8	7.63e-26	42	353	23	316	Non-reducing end alpha-L-arabinofuranosidase BoGH43A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02654 PE=1 SV=1
A7LXU0	4.27e-22	41	418	26	367	Non-reducing end alpha-L-arabinofuranosidase BoGH43B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02656 PE=1 SV=2
P45982	6.69e-20	44	370	6	329	Xylosidase/arabinosidase OS=Butyrivibrio fibrisolvens OX=831 GN=xylB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000483	0.998494	0.000357	0.000236	0.000218	0.000195

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004132_01986.