CAZyme Information: MGYG000004133_01690

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; HGM11514; HGM11514
• CAZyme ID: MGYG000004133_01690
• CAZy Family: GH2
• CAZyme Description: Beta-glucuronidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
587	MGYG000004133_47|CGC1	68143.31	4.7271

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004133	2160156	MAG	United Kingdom	Europe

• Gene Location: Start: 1981; End: 3744 Strand: +

Full Sequence      

MKNIPRPEHPRPQFMREKWINLNGEWEFEFDEREIGRELHFEKRNSLDMKITVPFCPESV
LSGIGDVNFHNAVWYRKDIEIPEDWKGKSVILHFGAVDYHAIVYVNSELVGEHKGGYSSF
EFDISPYLNDEQNYITVCAIDHVREMLPSGKQSERNNSYGCFYTRTTGIWQTVWLECAPK
ARVSSAKIFTDVQGGKVEIRLNKIGDTKNLKCKATAFWEGNAVGSGEVILNELQPSVNIE
LSENHPWEIGKGGLYDLIIETFDENKKVDEIKSYFGLRSVDLRERAFCINGKAIFGRWVL
DQGFYPDGIYTAPTDEALKNDIIYSQMLGFNGARLHEKIFEERFLYWADKLGYMVWGEHA
NWGYSPENLSELNNFLPEWMEALGRDFNHPAIIGWCPFNETWANKKENDGVIVEYPQSRE
VIETVYKITKALDKTRPVIDTSGNYHTTTDIFDVHDYEGNVEKFEENYAHIDEGIVNDQV
NRSSLSKWQKYRGEPLFMSEYGGIGWDISSDGWGYGNAPKTEDEFFERYRGLTEAILNNG
HFLGFCYTQLYDVEQEKNGLMTYDRKFKFDPKKFYEINTQIAEIEKE

Enzyme Prediction     

No EC number prediction in MGYG000004133_01690.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	13	559	2.1e-94	0.5904255319148937

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	9.36e-49	21	502	14	510	beta-D-glucuronidase; Provisional
COG3250	LacZ	4.31e-36	12	454	6	444	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	2.64e-18	51	438	99	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	3.37e-18	22	179	4	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
PRK09525	lacZ	4.29e-09	75	501	124	539	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALS26546.1	4.27e-216	4	587	9	588
AWP26385.1	9.87e-213	4	585	6	579
ANA78861.1	1.40e-212	4	585	6	579
AVV57223.1	1.40e-212	4	585	6	579
QLG42947.1	3.12e-211	1	585	1	578

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	7.89e-80	7	582	27	583	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
4JHZ_A	3.78e-32	22	502	17	508	Structureof E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12],4JHZ_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 2-[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]-N-[(1S,2S,5S)-2,5-dimethoxycyclohexyl]acetamide [Escherichia coli K-12]
3LPF_A	3.84e-32	22	502	17	508	Structureof E. coli beta-Glucuronidase bound with a novel, potent inhibitor 1-((6,7-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(3-methoxyphenyl)thiourea [Escherichia coli K-12],3LPF_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 1-((6,7-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(3-methoxyphenyl)thiourea [Escherichia coli K-12],3LPG_A Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 3-(2-fluorophenyl)-1-(2-hydroxyethyl)-1-((6-methyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)urea [Escherichia coli K-12],3LPG_B Structure of E. coli beta-Glucuronidase bound with a novel, potent inhibitor 3-(2-fluorophenyl)-1-(2-hydroxyethyl)-1-((6-methyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)urea [Escherichia coli K-12],5CZK_A Structure of E. coli beta-glucuronidase bound with a novel, potent inhibitor 1-((6,8-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(4-hydroxyphenyl)thiourea [Escherichia coli K-12],5CZK_B Structure of E. coli beta-glucuronidase bound with a novel, potent inhibitor 1-((6,8-dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-1-(2-hydroxyethyl)-3-(4-hydroxyphenyl)thiourea [Escherichia coli K-12]
3K4A_A	5.16e-32	22	502	17	508	Crystalstructure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12],3K4A_B Crystal structure of selenomethionine substituted E. coli beta-glucuronidase [Escherichia coli K-12]
6LEM_B	9.09e-32	22	502	13	504	ChainB, Beta-D-glucuronidase [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P05804	5.05e-31	22	502	15	506	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
P77989	4.99e-24	21	462	18	438	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P12265	6.61e-21	22	502	41	538	Beta-glucuronidase OS=Mus musculus OX=10090 GN=Gusb PE=1 SV=2
O18835	1.17e-20	9	502	29	541	Beta-glucuronidase OS=Canis lupus familiaris OX=9615 GN=GUSB PE=1 SV=1
P26257	1.32e-20	18	503	3	465	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000045	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004133_01690.