CAZyme Information: MGYG000004168_01008

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74; Firm-11
• CAZyme ID: MGYG000004168_01008
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
653	MGYG000004168_6|CGC1	74075.1	4.8269

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004168	4047630	MAG	United Kingdom	Europe

• Gene Location: Start: 20121; End: 22082 Strand: +

Full Sequence      

MKKKLEPLSLRCVILKDGLLAVRQTVNQNVTVPVVLEKCEVTGRVEAILHRWDGRPESRP
HPFWDSDIGKTLESVAYALISRRDGKLEEKADTIIELMVNAQEEDGYFNSYYQTCEPRQN
RWSNLYYMHELYCMGHLIEGAVAYYNATGKDAFLKMMCRYADLACSLFLDGGAHFGGYCG
HPEIELALVRLYETTGVDRYLELSRTFIEQRGQEPWFFEQESLKRGVDTEKTANLKRHLK
FFLPSRGPYAEYQAHLPVRQQAEPVGHAVRAMYLLSGIADIADLTDDDGLYTAARNVWDT
MVHTQYAITGGIGTASDGERFTFAYDLPNENTYNETCASVALIMAGLRLLQFGPDSSVAD
IIEQTLYNTILASVSLSGDSFFYANYLDVCPERFEHASKAMIDKMYAQRQEWFDVACCPP
NVARLMGSLGQYVASTDVKDDLYVHLYTGCELSYTVGGKEMHVEMLTQYPWKGEVTLILH
GEAPRVGTVWVRIPGWCQSWTVCLNGDRVESCLVNGYMPVSNRWTEGDTITVALEMYPVV
MEADPRVRQDAGKVALMRGPVVYCLEEADNGGNLHDVSMDAYSQIKEREICLEGLPVVTL
ELEARRYHRDIWEAGQLYRPVQKQEESVTLTAVPYFTWGNRGFGEMTVWINRE

Enzyme Prediction     

No EC number prediction in MGYG000004168_01008.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	15	564	5.3e-180	0.9942748091603053

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	1.83e-173	10	653	9	588	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	8.94e-138	13	564	2	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG4225	YesR	0.003	131	219	42	119	Rhamnogalacturonyl hydrolase YesR [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQO07828.1	2.02e-249	1	652	1	649
AGE22473.1	1.37e-172	63	653	69	643
AWO76529.1	4.97e-172	63	653	66	640
AGT32221.1	5.84e-172	63	653	66	640
QOR85903.1	8.52e-172	63	653	72	646

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	6.78e-170	63	653	74	648	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	1.10e-165	63	653	74	648	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
6EX6_A	4.48e-120	5	650	7	623	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
3WRE_A	5.95e-112	63	652	73	658	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	7.98e-112	63	652	73	658	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	3.26e-111	63	652	73	658	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000071	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004168_01008.