CAZyme Information: MGYG000004172_01476

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Rikenellaceae
• CAZyme ID: MGYG000004172_01476
• CAZy Family: GH28
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1242	MGYG000004172_5|CGC4	139027.24	6.6085

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004172	3050906	MAG	United Kingdom	Europe

• Gene Location: Start: 195723; End: 199451 Strand: -

Full Sequence      

MRRTVPISSAWCVRISAPTSDVTAIRAAIDACTQSGGGRVLVPEGLYFVKGPVVLKSHVE
LHLAEGAELRFSSDEKDYLPAVPTRWEGTEVFNYSPLIYAYHVNNIGVTGKGVVNGQGTK
NFATWKPGQKSDQRALRRMGTEVRPLSERLFGEGHRLRPALLEPVGCSDVLIEGITLVDA
PFWVIHPVSCENVTVRGVTVYSLNQNSDGCDPESSVNVLIEDCNFTTGDDGIAIKSGRDN
DAWRIGQPTENVLVRNCTFRSRTNGVCIGSEISGGVRNVCVMDCDIPLSRNGIYFKSNLD
RGGYVENVRVKGIRADTLGEALIRFESDYKSESRENYPTRFRGFLFEEMTCGVAGQCGIE
IGGFAALPVREVTVRDVMLGRTPEPLRINHAEEVELSGVTMNGRPVVYNTRPDRCISLDG
RWNVAISDGEPAVFGSEVPVPGVITQAVPSLGEDLDANGTDTVGYDYVWYLREFEVRGAE
NYPRAVLKLRAKYNAKVFLNGKEIGEDLHTTYSHGTFDVSDALVRNGKNRLVVRVGSWNT
ATSPSKENSAEWWRNSRAPGIWDDVTLELGQNVSVKHMKILPDLKSSSTECVVTVDNRER
GSQTVKVLAAVMDGDREVSRAETLLEVPAVGDGQAVLKVPSGMLRPWSPGKEGTPKLYRM
TVAVTASDGRLLSRKSDDFGYRSIEVAGRDVLLNGKKILFRAENVAFVRALSRWADAVFD
EAWIRAFLRAAVQEYNFNYLRIHLGHAYGKWYDIADQEGIMLQDEWRYMHDDEPVGKDRE
DAVTEFTRWVEQNVNHPSIVTWDQENEGNVKLEELKAALRRYDPTRLWGEDDFEARHIYE
YTENIVKSPEHAISGTKPSTVLESCRLWTNEYGLLEPRENFKTSRTASGWGLFNYTREDV
ARLLADLHADLGTFYRSRRIQAWAPFALLSGAVNGHNFYKGNIADSLSPQPNLLVLKKLN
EPVGTSVEMLQAREWYKDKTLYAPGRRYAKKVWVWNDRNEPVEVELTVGLANEAGETLSV
QRQRLTVPASGAVARELSFTMPREEGVYRIEPSLTLPDSVRIGGPGRRLMVARRLTSRIE
EYMAFGGRRKPVEGSRSLMEHFIGREVPLQVQYRIEEALQGGLLDKMEYSDDPARMYTVQ
STYYYDRTRQDITTLVFDKDGTVVSSTKAEAMPYVSLPAPIKKTIVEVIGKVPVDESKIT
RRRAGADSLYEISMIGSDLKYKLTISDSGELKDKKVSRKKSR

Enzyme Prediction     

No EC number prediction in MGYG000004172_01476.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	28	381	1.5e-73	0.9261538461538461
GH2	407	997	3.8e-63	0.6675531914893617

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5434	Pgu1	3.19e-70	19	339	96	421	Polygalacturonase [Carbohydrate transport and metabolism].
COG3250	LacZ	1.32e-24	416	873	13	476	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam00295	Glyco_hydro_28	6.64e-22	32	405	1	307	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
PRK10340	ebgA	2.16e-20	496	967	140	606	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	8.29e-17	404	826	3	443	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADY53936.1	0.0	24	1236	67	1275
QUT26779.1	9.47e-134	28	403	79	454
QUT89399.1	7.12e-133	24	418	65	459
ALJ59565.1	9.95e-133	24	418	65	459
SDT40105.1	4.89e-118	29	403	74	449

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5OLP_A	5.14e-69	21	400	60	447	Galacturonidase[Bacteroides thetaiotaomicron VPI-5482],5OLP_B Galacturonidase [Bacteroides thetaiotaomicron VPI-5482]
3JUR_A	1.05e-65	28	403	50	433	Thecrystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_B The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_C The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_D The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima]
1BHE_A	6.72e-17	6	379	9	361	ChainA, POLYGALACTURONASE [Pectobacterium carotovorum]
1YQ2_A	1.70e-16	496	808	152	444	ChainA, beta-galactosidase [Arthrobacter sp. C2-2],1YQ2_B Chain B, beta-galactosidase [Arthrobacter sp. C2-2],1YQ2_C Chain C, beta-galactosidase [Arthrobacter sp. C2-2],1YQ2_D Chain D, beta-galactosidase [Arthrobacter sp. C2-2],1YQ2_E Chain E, beta-galactosidase [Arthrobacter sp. C2-2],1YQ2_F Chain F, beta-galactosidase [Arthrobacter sp. C2-2]
3IAQ_A	6.72e-14	517	826	171	482	ChainA, Beta-galactosidase [Escherichia coli K-12],3IAQ_B Chain B, Beta-galactosidase [Escherichia coli K-12],3IAQ_C Chain C, Beta-galactosidase [Escherichia coli K-12],3IAQ_D Chain D, Beta-galactosidase [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7PZL3	2.42e-23	34	414	94	458	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1
Q47077	2.71e-17	497	826	154	484	Beta-galactosidase OS=Enterobacter cloacae OX=550 GN=lacZ PE=3 SV=1
Q2XQU3	4.68e-17	517	826	173	484	Beta-galactosidase 2 OS=Enterobacter cloacae OX=550 GN=lacZ PE=3 SV=1
Q59140	1.05e-16	397	808	13	435	Beta-galactosidase OS=Arthrobacter sp. (strain B7) OX=86041 GN=lacZ PE=1 SV=1
B4S2K9	1.07e-16	497	807	152	460	Beta-galactosidase OS=Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype) OX=1774373 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000049	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004172_01476.