CAZyme Information: MGYG000004174_00645

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74; Firm-11
• CAZyme ID: MGYG000004174_00645
• CAZy Family: GT84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2598	MGYG000004174_13|CGC1	288713.81	8.3973

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004174	2366989	MAG	United Kingdom	Europe

• Gene Location: Start: 19881; End: 27677 Strand: -

Full Sequence      

MQWIVAAAGLLILLLLALAVRPRRKPGYRYESLADSLGEHLRLTAARMECRGYARLSMRR
GMNGALERAVGYLNTLPEAELLPSGRWLCDNGRFLQEEIASMRLALQQAPRLRRVRGGPP
RIRLLARELVGHSAAHVDAQRLREAVAAWQEAAPLDTRELDALPLALQLTLTEIITDLAI
RCAIEQRARMNAVKVASLMRRGREKQFLRLFARYEKDPAFLERLVSELRAQDDVTCAAWL
DRYFGSHDLSAGKLAQSEHEHQTESCLWLSNAIVSLRSVDRMPWHRLCEELSLIHAQLCA
DAVYPRMDRESRAYYRMRLSRLSVSAKRPEQTVCNAALDLAAAGTDEARKHVGYYLLDRG
RRALLRSLRAYGLKSRLRLWVEEHASVLMRLTGWTAFAAIAAALSQTGWTPALWLALSAV
LAHAARNLALAACRRLVPPRMTPRMRVPSLQEETRTLVVCPTMLLDSAHALAMVKHLSVL
RQANRDPRLDFMLLGDFRDSLTANETGDADIVRTAAAATQALCEDTGHAFYYMQRERVFS
PRDQTHMSRERKRGSLETLFRLICGRPVEERFAYCSLPPESLRGRYRYVIALDSDTLLPP
GSALRLVGAMLHPLNRRRVEQGRMRGVSIVQPRMEIAAHTVKSWLSLWLGGSGGSEPYNA
LVADFYQDACGRGTFMGKGIIDPEAFLQATEGRILPGTVLSHDLLEGELAGCALASDITL
YDGHPRDLRGFMFRLHRWTRGDWQLLPYVLLPWRKEATRGALDAHARHRVRHNMLRSLIA
PLRVAVMLLAVGLGQPWALLAALVVPELPYLLRPDGRALAGCLCRLAVLPVEAGVQADAA
VRALYRLFVSHRRLLQWTTAAQTMRERARPPMSLFYLSMGAAAAVAAVGWLPGGCAACGV
ALGAWWALMPFALPLMERPYQKTQRPTEFMRETLLRLAQETWRFFDTAITPRDHDLPPDN
VQIEPNKGVTHRTSPTNIGLYLVSLLAAERLRILSPQETAERMERTVETLEALPKWKGHL
NNWYDTTTLQPLSPPFVSTVDSGNLAVCLLTCAQGVRVLMPELAARFSSLAARLDALASE
MDFAALYDADAGLFYVGMHTDRSQPTAEHYDLLASEARMTSFAAILMGQVPLRHWYRLGR
MFARTPGGKALVSYSGTMFEYMMPLLFQPPVRGTLLYNACAEAFRLQRRHRVGGVFGVSE
SGYYAFDPNLLYQYRAFGLPSLALAPDNQSDVVAPYATLLAAPMGARKAFANVLRLRNLG
MEGPLGLFEAADFCRARTGKPMRIVRSHMAHHQGMILLAVCNLLEEDYIATLFSSLPRVE
AYRLLLEEKPLRARGLVRRPFKQARRETPVVPLHAAYHAQPLRFPVEAHVLGGAGTTLFA
DARGGGYLQRDGVMLTRFQESCHLPSGPRFYLRDSRSGANWCVTDPAFVREVTFETGQVR
YVHERFGVEGTLRAYVNPLDGAVIHLLTLRNLSGAAREMEVCSYLEPALASREDDAAHPA
FQNLFIRTAKLNPNGVEAVRRSRGEEPERRLWHRLCADVPFQAFCVQTDRTSFLGRNRGV
ESPRELDLPMEAVAGEVGDVIEPCLSLRARFSLPNGAQARLAFVTALPNDNDTPEAFGDR
YAQPEAAARCYESALTRGLVTARYLSLSAEAQTLVWRLAGCVGYTGQPWQFRYALDNALP
LRGLWALGISGDYPILLVECHTEQELDLARLALKAHAYFRLNGLRMDLALVCAQPSGYVR
PLRDALSNLAGVSHSHDLIGKPGGVHLFDPSGVTPEQLALLRAAARLVLQAGQGGLKEQL
AKLDTALPQGGLATGHTSWKAELPPLEALEFGNGYGGFTPGEGDYVIDLPPGRETPAPWC
NPLCSERFGTLAGESGLVFSYAANSQAGRLTRWVNDSVLPQGEENVFVRDDENGLLWSLT
RLPLGLNLPVRVRHAPGCTEYETSGYGLYARMQCFTDGGIGARAIHLKNEGTDARTLTLM
HAAVFTIGDRPNAWQLCSLSREEGGVLVAHPHLDGPACLCALDGEPQRTATMSVGAYSGL
WGSAPAALRGALPCDAGNVGVLCYRLTLAPGETRTIVSALCHAPSRACLRDEKQRFCAQG
ASRRLYEVRALWEGRLDGLRFDLPDRALQLMLGRWLPYQVRASRLWMRAGFYQAGGAVGF
RDRLQDLLCLMPTEPDVVRAMLLDAAAHQFEEGDVQHWWHPPRYGVRTRCSDDLLFLPFV
CAQYVTVTGDETLLEESVPYLREPPLAQGEASRLTEPQVSDVSESVRLHCLRAIARVGLG
AHGLPLMGGGDWNDGMDRVGGENGESVWLGMFLCEVLRRFAPLCGEADRERLEADRRRLM
AGLDRYAWDGGWYLRAWYGDGTRLGGAGSEECRIDLLPQAWGVMCGVNRDRCASAMDQAW
RLLYDRSAGLMRLFAPPFDGVEQPGYIAGYLPGVRENGGHYAHAAAWAVSALYQLGMDTR
AWELARAMLPINRSLNRRQAALYRTEPYAMAADIYANPQQRGRGGWTWYTGSASWYQHVL
LTELLGFQKEGTTLRFRPVVPPGWDELRLTYRFGSATYHLRACRDCAAAMADGERLADGR
LTLADDGRIHEAVFPLRQ

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH94	1546	2592	4.5e-245	0.9961389961389961
GT84	1109	1316	1.1e-65	0.986046511627907

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3459	COG3459	0.0	1546	2598	2	1055	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
pfam17167	Glyco_hydro_36	7.57e-93	2112	2528	2	425	Glycosyl hydrolase 36 superfamily, catalytic domain. This is the catalytic region of the superfamily of enzymes referred to as GH36. UniProtKB:Q76IQ9 is a chitobiose phosphorylase that catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. The full-length enzyme comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain. The alpha-helical barrel component of the domain, this family, is the catalytic region.
cd11753	GH94N_ChvB_NdvB_2_like	2.70e-49	1313	1629	5	332	Second GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This second of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.
pfam06165	Glyco_transf_36	3.46e-42	1367	1605	4	246	Glycosyltransferase family 36. The glycosyltransferase family 36 includes cellobiose phosphorylase (EC:2.4.1.20), cellodextrin phosphorylase (EC:2.4.1.49), chitobiose phosphorylase (EC:2.4.1.-). Many members of this family contain two copies of this domain.
cd11756	GH94N_ChvB_NdvB_1_like	1.98e-40	1828	2096	1	283	First GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This first of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVX19642.1	0.0	78	2590	61	2760
AVX30049.1	0.0	78	2590	61	2760
ABC82952.1	0.0	83	2593	90	2737
ACG74515.1	0.0	83	2591	90	2730
ABS28204.1	0.0	83	2590	87	2718

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3QDE_A	1.59e-52	1835	2591	3	805	ChainA, Cellobiose phosphorylase [Acetivibrio thermocellus],3QDE_B Chain B, Cellobiose phosphorylase [Acetivibrio thermocellus]
1V7V_A	1.15e-51	1835	2561	3	760	Crystalstructure of Vibrio proteolyticus chitobiose phosphorylase [Vibrio proteolyticus],1V7W_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc [Vibrio proteolyticus],1V7X_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate [Vibrio proteolyticus]
3S4C_A	3.66e-43	1835	2560	3	781	Lactosephosphorylase in complex with sulfate [Cellulomonas uda],3S4D_A Lactose phosphorylase in a ternary complex with cellobiose and sulfate [Cellulomonas uda]
3RRS_A	2.00e-42	1835	2560	3	781	Crystalstructure analysis of cellobiose phosphorylase from Cellulomonas uda [Cellulomonas uda],3RRS_B Crystal structure analysis of cellobiose phosphorylase from Cellulomonas uda [Cellulomonas uda],3RSY_A Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol [Cellulomonas uda],3RSY_B Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol [Cellulomonas uda],3S4A_A Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose [Cellulomonas uda],3S4A_B Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose [Cellulomonas uda],3S4B_A Cellobiose phosphorylase from Cellulomonas uda in complex with glucose [Cellulomonas uda],3S4B_B Cellobiose phosphorylase from Cellulomonas uda in complex with glucose [Cellulomonas uda]
2CQS_A	2.18e-41	1835	2560	23	801	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQS_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQT_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],2CQT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],3QFY_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFY_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFZ_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QFZ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20471	5.29e-317	79	2565	84	2808	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2
B9K7M6	3.74e-52	1835	2590	3	806	Cellobiose phosphorylase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E) OX=309803 GN=cbpA PE=1 SV=1
Q76IQ9	5.86e-51	1835	2561	3	760	N,N'-diacetylchitobiose phosphorylase OS=Vibrio proteolyticus OX=671 GN=chbP PE=1 SV=1
Q9F8X1	2.46e-50	1835	2560	3	759	N,N'-diacetylchitobiose phosphorylase OS=Vibrio furnissii OX=29494 GN=chbP PE=1 SV=1
Q8P3J4	1.81e-37	1907	2593	94	785	Cellobionic acid phosphorylase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=XCC4077 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000059	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
3	20
784	806
874	908