CAZyme Information: MGYG000004185_01276

Basic Information

• Species: Bacteroides sp900553815
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides sp900553815
• CAZyme ID: MGYG000004185_01276
• CAZy Family: GH9
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
840		95322.03	6.2809

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004185	3289740	MAG	United Kingdom	Europe

• Gene Location: Start: 54687; End: 57209 Strand: -

Full Sequence      

MKNRKTLLALSALCLALTANADEWIRINQLGYLPQSVKVAVFMSEEAVDLHEYSLIDDYT
GQVVETFRAPQPTGRYGNMQSTYRLNFSQFTRPGTYRLKAGEALSPSFPINTRVYDGTAD
FLLNYMRQQRCGYNPYLKDSCHVHDGYIVYHPTKTGQHIDVRGGWHDATDYLQYTTTSAN
AIYQMMFAYQQNPESFADAYDADGKPGANGIPDIVDEIKWGLDWLNRMNPAKGELYNQIA
DDRDHAGMRLPNKDKVDYGYGPGNGRPVYFCSGEPQQRGKYTNATTGVASTAGKFASCFA
LGASVLKDFYPEFAEEIGRKAADAYEEGVKRPGVCQTASVLSPYIYEEDNWTDDMELGAM
ELYHKTGNEAYLKQALEYGRREPVTPWMGADSARHYQWYPFMNMGHYWLARSGNERISRE
FIRNLRAGIVRTHEKAVESPFLHGIPYIWCSNNLTSAMLTQCRLYRELTADSTYQEMETA
LLDWLLGCNPWGTSMIVELPLHGDYPSQPHSSLLNAGAGNTTGGLVDGPVYRSIFESLRG
VNMTGIPGVLGQDYERFQPDLMVYHDALHDYSTNEPTMDGTASLTYYLSAMQKEGMRQGG
GNTSHNVSHDGCITRTDPAKKQLTLVFTAHDKADGAADILHTLKKYRIKGAFFFTGRFFE
LYPEVVKQLKADGHYVSSHSYGHLLYASWEDRDSLLVSRQEFEDDLRRSFEVMRPFGITP
QEAPFFIPPYEYYNRQVSAWAQAMGLQLINFTPGTLTNADYTTPDMKNYRSSKEILNKVM
EVEKKEGLNGHLMLVHFGTDDRRTDKFYKRSLEQLIRQLQRKGYRFVPLTEALGYGQSQE

Enzyme Prediction     

No EC number prediction in MGYG000004185_01276.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH9	115	588	2.7e-75	0.992822966507177
CE4	623	749	4.9e-17	0.8923076923076924

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00759	Glyco_hydro_9	1.47e-62	123	588	7	374	Glycosyl hydrolase family 9.
cd10917	CE4_NodB_like_6s_7s	9.45e-28	621	820	1	171	Catalytic NodB homology domain of rhizobial NodB-like proteins. This family belongs to the large and functionally diverse carbohydrate esterase 4 (CE4) superfamily, whose members show strong sequence similarity with some variability due to their distinct carbohydrate substrates. It includes many rhizobial NodB chitooligosaccharide N-deacetylase (EC 3.5.1.-)-like proteins, mainly from bacteria and eukaryotes, such as chitin deacetylases (EC 3.5.1.41), bacterial peptidoglycan N-acetylglucosamine deacetylases (EC 3.5.1.-), and acetylxylan esterases (EC 3.1.1.72), which catalyze the N- or O-deacetylation of substrates such as acetylated chitin, peptidoglycan, and acetylated xylan. All members of this family contain a catalytic NodB homology domain with the same overall topology and a deformed (beta/alpha)8 barrel fold with 6- or 7 strands. Their catalytic activity is dependent on the presence of a divalent cation, preferably cobalt or zinc, and they employ a conserved His-His-Asp zinc-binding triad closely associated with the conserved catalytic base (aspartic acid) and acid (histidine) to carry out acid/base catalysis. Several family members show diversity both in metal ion specificities and in the residues that coordinate the metal.
cd10950	CE4_BsYlxY_like	3.40e-26	616	833	1	188	Putative catalytic NodB homology domain of uncharacterized protein YlxY from Bacillus subtilis and its bacterial homologs. The Bacillus subtilis genome contains six polysaccharide deacetylase gene homologs: pdaA, pdaB (previously known as ybaN), yheN, yjeA, yxkH and ylxY. This family is represented by Bacillus subtilis putative polysaccharide deacetylase BsYlxY, encoded by the ylxY gene, which is a member of the carbohydrate esterase 4 (CE4) superfamily. Although its biological function still remains unknown, BsYlxY shows high sequence homology to the catalytic domain of Bacillus subtilis pdaB gene encoding a putative polysaccharide deacetylase (BsPdaB), which is essential for the maintenance of spores after the late stage of sporulation and is highly conserved in spore-forming bacteria. However, disruption of the ylxY gene in B. subtilis did not cause any sporulation defect. Moreover, the Asp residue in the classical His-His-Asp zinc-binding motif of CE4 esterases is mutated to a Val residue in this family. Other catalytically relevant residues of CE4 esterases are also not conserved, which suggest that members of this family may be inactive.
pfam02927	CelD_N	1.48e-25	24	103	2	81	Cellulase N-terminal ig-like domain.
cd02850	E_set_Cellulase_N	9.97e-24	24	110	1	86	N-terminal Early set domain associated with the catalytic domain of cellulase. E or "early" set domains are associated with the catalytic domain of cellulases at the N-terminal end. Cellulases are O-glycosyl hydrolases (GHs) that hydrolyze beta 1-4 glucosidic bonds in cellulose. They are usually categorized into either exoglucanases, which sequentially release terminal sugar units from the cellulose chain, or endoglucanases, which also attack the chain internally. The N-terminal domain of cellulase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT35459.1	0.0	1	834	1	835
QUT66756.1	0.0	1	834	1	835
QPH59586.1	0.0	1	834	1	835
QUT98069.1	0.0	1	834	1	835
QBJ19309.1	0.0	1	834	1	835

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3X17_A	6.75e-34	25	586	18	551	Crystalstructure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium],3X17_B Crystal structure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium]
5U2O_A	1.12e-27	33	594	2	542	Crystalstructure of Zn-binding triple mutant of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]
5U0H_A	2.49e-24	33	594	2	542	Crystalstructure of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]
6DHT_A	3.92e-22	22	591	15	564	Bacteroidesovatus GH9 Bacova_02649 [Bacteroides ovatus ATCC 8483]
4CJ0_A	1.14e-21	22	510	27	492	ChainA, ENDOGLUCANASE D [Acetivibrio thermocellus],4CJ1_A Chain A, ENDOGLUCANASE D [Acetivibrio thermocellus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXT3	1.32e-21	10	591	13	578	Xyloglucan-specific endo-beta-1,4-glucanase BoGH9A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02649 PE=1 SV=1
A3DDN1	5.09e-21	22	617	51	596	Endoglucanase D OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celD PE=1 SV=1
P0C2S4	6.25e-21	22	510	27	492	Endoglucanase D (Fragment) OS=Acetivibrio thermocellus OX=1515 GN=celD PE=1 SV=1
P23658	2.25e-18	27	591	6	545	Cellodextrinase OS=Butyrivibrio fibrisolvens OX=831 GN=ced1 PE=1 SV=1
P14090	2.58e-17	25	595	341	911	Endoglucanase C OS=Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) OX=590998 GN=cenC PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000290	0.998906	0.000248	0.000180	0.000171	0.000157

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004185_01276.