CAZyme Information: MGYG000004200_00133

Basic Information

• Species: F23-B02 sp000431075
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; F23-B02; F23-B02 sp000431075
• CAZyme ID: MGYG000004200_00133
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
771		86526.86	5.8814

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004200	1810687	MAG	United Kingdom	Europe

• Gene Location: Start: 120783; End: 123098 Strand: +

Full Sequence      

MTIPFNNDWLFTSDYDAGFDNAETVRIPHNVREIPYNYIDCQDYQMLCGYKKSFFVPEDW
QGKRMLLRFDGAAHDATVYCNSKLAAHHACGYTAFTADLTEFLKFGQENTVTVRLDTREN
LNIPPFGFVIDYLCYGGLYRDVELEVRNSTYIKNVLIENTGLRDISVSIDTSGRLKVGKL
VAEIFDADGKVAASAMGAKFKLTVPDGKLWSPDNPYLYTLKAKIVDGDTVLDEKSYRFGL
RTAKFENNGFYLNGKKMKLRGLNRHQSFAYTGYAMPKSMQRLDADILKNELGVNAVRTSH
YPQSQHFIDRCDELGLLVFTEIPGWQHIGNDAWKDQAVENTREMVTQYMHHPSIILWGVR
INESQDDDGLYKRTNAVAHELDRSRQTSGVRYIEKSSLLEDVYAFNDFSHSGNNAGLKAK
NSVTSNKRRPYLVSECNGHMFPTKAFDDERHRLSHALRHAKVLDAMYAADDICGIFPWCM
FDYNTHKDFGSGDGVCYHGVMDMFRNPKLAATVYASQSEENPCCVVSSSMDIGEHPAGDI
GSVYVFTNADSVRLFKNGLLVKEFYPDSESYPALPHPPVVIDDFIGDLLETQESFDAKTA
EQLKECLYAVAKYGTSALPPKYLLKLGKLMLLKGLKFSDGVELYGKYVSNWGGEATEWRF
DAVKNGEVVKSVIKKPGQKAHIEVEVSSTELYEGDTYDVAAVRIYARDELGNSAVYYQEP
VILSCSDGLEIIGPHVISLKGGCGGTYVKTTGTAGNAELMINDIKVDFIIK

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	516	1.7e-106	0.6143617021276596

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	8.48e-81	1	519	11	565	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	3.75e-61	50	385	69	443	beta-D-glucuronidase; Provisional
PRK10340	ebgA	3.32e-41	50	364	113	450	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	4.24e-25	50	364	124	463	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	6.58e-20	250	385	8	156	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCN30094.1	9.92e-309	1	767	1	803
AFN57653.1	9.87e-305	1	761	1	768
VCV21695.1	7.54e-302	3	767	5	793
CBL11718.1	4.63e-300	3	767	6	801
AEN96401.1	6.79e-299	3	767	5	786

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EUV_A	6.50e-169	1	759	3	714	ChainA, Beta-D-galactosidase [Paracoccus sp. 32d],5EUV_B Chain B, Beta-D-galactosidase [Paracoccus sp. 32d],5LDR_B Chain B, Beta-D-galactosidase [Paracoccus sp. 32d]
5LDR_A	6.70e-169	1	759	4	715	ChainA, Beta-D-galactosidase [Paracoccus sp. 32d]
6MVG_A	1.91e-53	6	558	31	616	Crystalstructure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_B Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_C Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus]
6MVH_A	2.67e-53	3	558	28	616	Crystalstructure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_B Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_C Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_D Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis]
6ED1_A	1.13e-52	3	382	27	437	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei],6ED1_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Phocaeicola dorei]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26257	2.01e-187	3	582	5	612	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
P77989	5.90e-173	3	759	6	721	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
Q59750	1.32e-101	5	582	7	612	Beta-galactosidase OS=Rhizobium meliloti OX=382 GN=lacZ PE=1 SV=1
T2KPJ7	2.71e-55	6	573	57	684	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KM09	4.90e-44	50	559	110	662	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000063	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004200_00133.