CAZyme Information: MGYG000004224_00454

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; UBA1390; UBA1390
• CAZyme ID: MGYG000004224_00454
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
651	MGYG000004224_10|CGC2	74250.41	5.023

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004224	2221068	MAG	United Kingdom	Europe

• Gene Location: Start: 16143; End: 18098 Strand: -

Full Sequence      

MEKCQVKSPYAAARPMEPLQNHWTGGLWKERYDTCAQETVEFIRSLFEDPKIFHVVENFR
IAAGLQEGSFKGTPFGDGDFYKWMEGASYIAAANHDEKLLQEIDSYIELIGKAQLPDGYI
STKQIIGERQGNGVSRQGDINDFEVYNIGHLLTAACLHYKMTGKTNFMEIAEKAAQYLKN
LYEEAARTGEVKTAVCPSHYMGLIDMYRTSGNQEYLKLAELSINLRDSVKNGLDDNQDRI
PLRTHEKIVGHAVRSTYLYAGVADLYAETGEEALRTMLDKVWASCVQKKLYITGGVGALY
NGVSPYGNFWHDQKMHQAFGYEYQLPNVTAYNETCAALGNIFWNYRMFLLHPEAVYFDLI
ERTMLNVALAAISLEGKEFFYENMLRRAKQLDYELCWPLHRTGVISSFCCPPNLMRTIAE
SAWYAYVFSKDAVWTGIYGDCEAQVALENGADFTMRQETGYPYDGTIVLRAGEVRGDQAF
RLMVRIPGWVQSGYVEYQGKKMELSEKDSGTYLELMVDSPRDMVVKIVLDMPARMTEAHP
MVEECAGQVAVERGPLVYCMETPDAEVDTLDRVVLDADTEFETEEAEITGRRLLALRTQA
LLREPMGNSLYRTYKPEKTSTISVRLVPYYAWDNRSRDGEMKIWMPVRYRH

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	22	559	9.4e-157	0.9961832061068703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	1.39e-130	22	559	3	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	8.91e-103	25	645	20	585	Uncharacterized conserved protein, DUF1680 family [Function unknown].
cd04791	LanC_SerThrkinase	0.002	155	322	94	265	Lanthionine synthetase C-like domain associated with serine/threonine kinases. Some members of this subgroup lack the zinc binding site and the active site residues, and therefore are most likely inactive. The function of this domain is unknown.
COG1331	YyaL	0.010	149	220	414	494	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGC67292.1	7.13e-289	7	649	7	651
AGI38357.1	7.13e-289	7	649	7	651
ANX00320.1	2.03e-288	7	649	7	651
ANW97794.1	9.52e-287	7	649	7	651
QOV18624.1	1.28e-239	6	647	6	649

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MQO_A	1.07e-172	22	647	45	696	Glycosidehydrolase BT_1003 [Bacteroides thetaiotaomicron]
6EX6_A	8.32e-108	24	647	18	625	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	2.49e-106	28	645	23	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	4.69e-102	28	645	23	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
7EXU_A	1.26e-72	24	644	6	655	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.48e-71	24	644	6	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000036	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004224_00454.