CAZyme Information: MGYG000004233_01062

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Fusicatenibacter
• CAZyme ID: MGYG000004233_01062
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
750	MGYG000004233_21|CGC1	85210.95	4.9159

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004233	2952530	MAG	United Kingdom	Europe

• Gene Location: Start: 7173; End: 9425 Strand: -

Full Sequence      

MRNIMPFNSKWAFSKEADQVPESMPTRWVWVDLPHTWNGIDGQDGGGDYYRGTCYYAKEF
KKMDLPEGEEYYLEFCGANASADVYVNGKKLAHHDGGYSTWRVKITEELKDDNLVVVAVD
NGVNDRVYPQQADFTFYGGLYRKVSLIAVPKAHFELEYYGTPAIKVTPEITGKNAKVETE
VYVSGDMAGKTLVYTLKNQDGTVAETKTVDAGQTVAEFAMENVHLWNGRKDPYLYTMEVK
LMDGEEELDCVSTRFGCRTFHVDPEKGFFLNGESYPLRGVARHQDRPRIGNALLPEHHEE
DIELICEVGATTIRLAHYQHDQYFYDLCDEKGLVIWAEIPYISNHMPNGRENTISQMKEL
IVQNYNHPSIVVWGLSNEITMTGSSTPDLIENHKILNDMVHEMDKTRLTTMACVSMCDMH
DPYVQISDVVSYNHYFGWYGGDVSMNGPWFDKFHAEFPNKPIGISEYGCEALNWHASNPE
AGDYTEEYQAYYHEELIKQLYSRPYIWATHVWNMFDFAADARNEGGESGQNHKGLVTFDR
KYKKDAFYAYKAWLSDEPFVHICGKRYVDRVEDVTKVTVYSNQPEVELFANGKSLGKQTS
DVHFFYFEVPNAGETKLYAKAGACEDESMIRKVDTFNEAYRMKEKGAVLNWFDITAPEGY
LSLNDKIQDIAKTEQGAMFLNGLFKQIMEKMGGENKEKAEAAGKDGGAGLMSMMGSFTMI
RLLNLMGAAGGAMTKEEMLALNAQLNQIKK

Enzyme Prediction     

EC	3.2.1.31

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	2	552	2.5e-98	0.6236702127659575

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	5.75e-68	50	553	63	592	beta-D-glucuronidase; Provisional
COG3250	LacZ	3.84e-60	1	553	9	595	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	1.17e-34	68	528	125	559	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02836	Glyco_hydro_2_C	6.10e-31	268	553	8	297	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK09525	lacZ	1.95e-17	139	378	208	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEN97389.1	0.0	1	750	1	755
QJA08636.1	0.0	1	748	1	751
CED93924.1	0.0	1	749	1	751
ASM70256.1	0.0	1	750	1	737
CBJ55484.1	0.0	1	750	1	751

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6MVG_A	0.0	1	750	24	777	Crystalstructure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_B Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus],6MVG_C Crystal structure of FMN-binding beta-glucuronidase from Ruminococcus gnavus [[Ruminococcus] gnavus]
6MVH_A	0.0	1	750	24	778	Crystalstructure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_B Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_C Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis],6MVH_D Crystal structure of FMN-binding beta-glucuronidase from Roseburia hominis [Roseburia hominis]
7KGZ_A	1.78e-265	1	750	1	733	ChainA, Beta-glucuronidase [Roseburia hominis],7KGZ_B Chain B, Beta-glucuronidase [Roseburia hominis]
6MVF_A	1.15e-203	1	675	24	710	Crystalstructure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6],6MVF_B Crystal structure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6],6MVF_C Crystal structure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6],6MVF_D Crystal structure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6],6MVF_E Crystal structure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6],6MVF_F Crystal structure of FMN-binding beta-glucuronidase from Facaelibacterium prausnitzii L2-6 [Faecalibacterium prausnitzii L2-6]
3CMG_A	7.51e-136	31	603	28	620	Crystalstructure of putative beta-galactosidase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	6.49e-87	28	605	79	682	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	3.81e-47	2	598	3	584	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
A7LXS9	1.14e-46	7	602	47	702	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	5.33e-44	7	597	51	666	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P26257	6.50e-42	1	409	1	413	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000063	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004233_01062.