CAZyme Information: MGYG000004233_01253

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Fusicatenibacter
• CAZyme ID: MGYG000004233_01253
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1167	MGYG000004233_26|CGC1	131984.59	4.6997

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004233	2952530	MAG	United Kingdom	Europe

• Gene Location: Start: 23270; End: 26773 Strand: +

Full Sequence      

MKTETNRSMGYYGTGTHLFNDGWEFAKQPLFTSYEEVKQHPEAFVPVGLPHDWLIFQGTD
LYENGTGWYRKRFVYLPGEDEIAFLRFEGVYMDSVIYVNGEKAMEWKYGYSTFEVPLAGR
LKDGENEIMVGVNFQSPNSRWYSGAGIYRDVWLKVVKQTHLVSDGSYISVRPAGNGSWEL
EISTEGIFCQKGEIRYTLTERESGKAMPLDEKGAEALVVQAGAQEGIQMIASTAVIPKIK
CWSVEHPHLYDLKTELYLEGECVQAERDTIGFCTKEFRPDQGFLLNGSKVKLNGVCEHHD
LGCLGAAYNHRAMERKLEKLKAMGVNALRLTHNMFAPDVMELADSMGFLVVSEAFDMWER
PKNPYDYGRFFPEWYPKDVASWVRRDRNHPSLILWSIGNEIYDTHVSERGQEITKNLMRE
VRIHDPRKNAEITIGSNYMPWENAQKCADLVKVAGYNYSEKYYNEHHKAHPDWIIYGSET
ASTVQSRGIYHFPYSQSVLADVDEQCSALGNSTTSWGAKSSEACVTAERDHAFSCGQFLW
TGFDYIGEPTPYHTRNSYFGQLDTAGFPKDSYYIYQAEWTDYRKHPMVHLFPYWDFNEGQ
LIDVRVCSNAPLVELFMNGRSQGTFEIDHAHGSEKELSGHWQLPFEPGEITAKAYDEYGN
EIASETRYSFGEAEKISVKPDRTVLKADGRDLAFLEISMEDREGHPVENAANRVFVRVSG
KGYLAGLDNGDSTDREEYKTVSRRLFSGKLLAVVAADTEPGEIKVEISSRGMEPATVVLQ
AEACETEEGIGTDAWRVDESRAQKETEQYCEDIPVRALRLQASNGTILTPENAWTEVTAE
ICPENALDREVIWSVVDDAGIPSGLANVESQGNCAKITAVSDGDFRLRCMSKSGTQGVRI
ISEMNFSVNGFGKAYLDPYGFIAGGLYDYVQGTASNGNEHGVATSRDGETQVGFHNLDFG
PFGSDEIQLPIFALTDEDYEILVYEGMPGEEGSELIGELHYQKPSIWNTYQPETYRLKKR
LKGITSICFVLHAKIHLKGFSFTRLEKAYEKLLAVESDHLYGDSFCRKEGRVTEIGNNVT
LEYTDMNMGEQGASGIVVCGYTPMEKNMIQIRFTSEEEEITQIVEFCHSEQETEQEFSLQ
PVSGTWNVSFVFLPGSNFDFHWFRFVK

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	16	577	2.5e-97	0.6050531914893617

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.14e-54	33	595	50	614	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	2.24e-35	676	778	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	1.29e-33	65	463	65	473	beta-D-glucuronidase; Provisional
PRK10340	ebgA	2.42e-26	63	400	107	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	7.14e-22	20	400	56	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QJU15650.1	0.0	18	1167	2	1146
ASU31293.1	0.0	18	1167	2	1146
ANU74303.1	0.0	18	1167	2	1146
QQQ91838.1	0.0	18	1167	2	1146
AWP27978.1	0.0	18	1167	6	1152

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	2.88e-170	19	779	6	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	1.57e-167	8	772	1	798	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5T98_A	2.98e-152	8	782	11	821	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4CU6_A	2.14e-147	19	769	18	832	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	5.86e-147	19	769	18	832	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	2.49e-152	19	782	47	849	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	2.12e-88	2	765	41	804	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.06e-47	47	654	82	702	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	4.36e-35	19	769	8	725	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	9.94e-32	16	792	28	806	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000064	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004233_01253.