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CAZyme Information: MGYG000004240_00379

You are here: Home > Sequence: MGYG000004240_00379

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Collinsella sp900541145
Lineage Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Collinsella; Collinsella sp900541145
CAZyme ID MGYG000004240_00379
CAZy Family GH4
CAZyme Description 6-phospho-alpha-glucosidase
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
447 MGYG000004240_12|CGC1 50377.39 4.706
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000004240 1902578 MAG United Kingdom Europe
Gene Location Start: 18516;  End: 19859  Strand: +

Full Sequence      Download help

MSKKNYAVTI  AGGGSTFTPG  IALMLLEERD  RFPVNKVTFY  DNNAERQETV  AKACEIYFHE60
NAPEVEFNYT  TDPETAFTGT  DFVLAHIRVG  LYAMRELDEK  IPLKYGCVGQ  ETCGAGGIAY120
GMRSIGGVIE  ILDYMEKYSP  NAWMLNYSNP  AAIVAEACRV  LRPNSRIINI  CDMPIDLMDK180
MSRMCGIKDR  RELQYSYYGL  NHFGWWSKIY  DKEGNDLMPQ  IKEHMAKNGY  LDGIEHEAGK240
EQHVEESWIH  TFGKAKDVYA  VDPETIPNTY  LKYYLYPDYV  VETSDPNYTR  ANEVMDGREK300
KIFGACRDII  AKGTAKDGGF  EPDVHANYIV  DLACALAENT  LERFLLIVPN  DGAVPNFDPT360
AMVEVPCIVG  SNGFEKICQG  PIPQFQKGLM  EQQVSVEKLV  VQAWVEGSYQ  KLWQALTLSK420
TIPSASVAKQ  ILDELIEANK  DFWPELK447

Enzyme Prediction      help

EC 3.2.1.122

CAZyme Signature Domains help

Created with Snap224467891111341561782012232452682903123353573794024248184GH4
Family Start End Evalue family coverage
GH4 8 184 7.7e-68 0.9832402234636871

CDD Domains      download full data without filtering help

Created with Snap224467891111341561782012232452682903123353573794024246447GH4_GlvA_pagL_like3447CelF8438GH4_glycoside_hydrolases8442GH4_P_beta_glucosidase8187Glyco_hydro_4
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
cd05298 GH4_GlvA_pagL_like 0.0 6 447 1 437
Glycoside Hydrolases Family 4; GlvA- and pagL-like glycosidases. Bacillus subtilis GlvA and Clostridium acetobutylicum pagL are 6-phospho-alpha-glucosidase, catalyzing the hydrolysis of alpha-glucopyranoside bonds to release glucose from oligosaccharides. The substrate specificities of other members of this subgroup are unknown. Some bacteria simultaneously translocate and phosphorylate disaccharides via the phosphoenolpyruvate-dependent phosphotransferase system (PEP_PTS). After translocation, these phospho-disaccharides may be hydrolyzed by the GH4 glycoside hydrolases, which include 6-phospho-beta-glucosidases, 6-phospho-alpha-glucosidases, alpha-glucosidases/alpha-glucuronidases (only from Thermotoga), and alpha-galactosidases. Members of this subfamily are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
COG1486 CelF 0.0 3 447 1 440
Alpha-galactosidase/6-phospho-beta-glucosidase, family 4 of glycosyl hydrolase [Carbohydrate transport and metabolism].
cd05197 GH4_glycoside_hydrolases 3.68e-154 8 438 3 425
Glycoside Hydrases Family 4. Glycoside hydrolases cleave glycosidic bonds to release smaller sugars from oligo- or polysaccharides. Some bacteria simultaneously translocate and phosphorylate disaccharides via the phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). After translocation, these phospho-disaccharides may be hydrolyzed by GH4 glycoside hydrolases. Other organisms (such as archaea and Thermotoga maritima) lack the PEP-PTS system, but have several enzymes normally associated with the PEP-PTS operon. GH4 family members include 6-phospho-beta-glucosidases, 6-phospho-alpha-glucosidases, alpha-glucosidases/alpha-glucuronidases (only from Thermotoga), and alpha-galactosidases. They require two cofactors, NAD+ and a divalent metal (Mn2+, Ni2+, Mg2+), for activity. Some also require reducing conditions. GH4 glycoside hydrolases are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
cd05296 GH4_P_beta_glucosidase 8.94e-109 8 442 3 418
Glycoside Hydrolases Family 4; Phospho-beta-glucosidase. Some bacteria simultaneously translocate and phosphorylate disaccharides via the phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). After translocation, these phospho-disaccharides may be hydrolyzed by the GH4 glycoside hydrolases such as the phospho-beta-glucosidases. Other organisms (such as archaea and Thermotoga maritima ) lack the PEP-PTS system, but have several enzymes normally associated with the PEP-PTS operon. The 6-phospho-beta-glucosidase from Thermotoga maritima hydrolylzes cellobiose 6-phosphate (6P) into glucose-6P and glucose, in an NAD+ and Mn2+ dependent fashion. The Escherichia coli 6-phospho-beta-glucosidase (also called celF) hydrolyzes a variety of phospho-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, and gentobiose-6P. Phospho-beta-glucosidases are part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
pfam02056 Glyco_hydro_4 5.81e-64 8 187 2 183
Family 4 glycosyl hydrolase.

CAZyme Hits      help

Created with Snap224467891111341561782012232452682903123353573794024241446BCV17940.1|GH41446APF21054.1|GH41446QJU46558.1|GH41446QCJ08317.1|GH41446AXB87048.1|GH4
Hit ID E-Value Query Start Query End Hit Start Hit End
BCV17940.1 4.67e-295 1 446 25 470
APF21054.1 1.03e-273 1 446 1 443
QJU46558.1 1.03e-273 1 446 1 443
QCJ08317.1 1.03e-273 1 446 1 443
AXB87048.1 5.93e-273 1 446 1 443

PDB Hits      download full data without filtering help

Created with Snap2244678911113415617820122324526829031233535737940242444466DUX_A34461U8X_X44476VC6_A14455C3M_A44451S6Y_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
6DUX_A 1.20e-210 4 446 5 442
ChainA, 6-phospho-alpha-glucosidase [Klebsiella pneumoniae],6DUX_B Chain B, 6-phospho-alpha-glucosidase [Klebsiella pneumoniae],6DVV_A Chain A, 6-phospho-alpha-glucosidase [Klebsiella pneumoniae],6DVV_B Chain B, 6-phospho-alpha-glucosidase [Klebsiella pneumoniae]
1U8X_X 4.55e-208 3 446 26 464
CrystalStructure Of Glva From Bacillus Subtilis, A Metal-requiring, Nad-dependent 6-phospho-alpha-glucosidase [Bacillus subtilis]
6VC6_A 1.43e-129 4 447 2 441
2.1Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis],6VC6_B 2.1 Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis],6VC6_C 2.1 Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis],6VC6_D 2.1 Angstrom Resolution Crystal Structure of 6-phospho-alpha-glucosidase from Gut Microorganisms in Complex with NAD and Mn2+ [Merdibacter massiliensis]
5C3M_A 5.32e-58 1 445 1 436
Crystalstructure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_B Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_C Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5C3M_D Crystal structure of Gan4C, a GH4 6-phospho-glucosidase from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
1S6Y_A 2.28e-56 4 445 6 439
2.3Acrystal structure of phospho-beta-glucosidase [Geobacillus stearothermophilus]

Swiss-Prot Hits      download full data without filtering help

Created with Snap224467891111341561782012232452682903123353573794024244447sp|C7NB67|PAGL_LEPBD4446sp|Q97LM4|MALH_CLOAB4446sp|Q9AGA6|AGLB_KLEPN1446sp|P54716|GLVA_BACSU4447sp|O06901|MALH_FUSMR
Hit ID E-Value Query Start Query End Hit Start Hit End Description
C7NB67 4.51e-233 4 447 2 440
6-phospho-alpha-glucosidase OS=Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249 / C-1013-b) OX=523794 GN=pagL PE=1 SV=1
Q97LM4 6.31e-231 4 446 2 440
Maltose-6'-phosphate glucosidase MalH OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=malH PE=1 SV=1
Q9AGA6 2.33e-221 4 446 2 439
6-phospho-alpha-glucosidase OS=Klebsiella pneumoniae OX=573 GN=aglB PE=1 SV=1
P54716 1.52e-219 1 446 1 441
Maltose-6'-phosphate glucosidase OS=Bacillus subtilis (strain 168) OX=224308 GN=glvA PE=1 SV=1
O06901 1.08e-217 4 447 2 441
Maltose-6'-phosphate glucosidase OS=Fusobacterium mortiferum OX=850 GN=malH PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000066 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      download full data without filtering help

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