CAZyme Information: MGYG000004292_01193

Basic Information

• Species: UMGS1474 sp900552115
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; UMGS1474; UMGS1474 sp900552115
• CAZyme ID: MGYG000004292_01193
• CAZy Family: GH95
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1617		175806.16	4.4503

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004292	2231746	MAG	China	Asia

• Gene Location: Start: 7170; End: 12023 Strand: +

Full Sequence      

MAASMAILAVSVPMGQAAASVSAESLPADPVPLQTWYTQPAPMTADGWEQQATQLGNGLI
GAMVFGGVSREKIQVNEKSIWSGGPGADPNFNYGIQTDSDKAKAALNELRSILQKHATKF
TETQKAYIDGSGKLVTGNYSYGKDAARIAELEKELYGDRSTYGSYQTLGDIYLSDPSGND
DYTDYKRSLDLRRGVATIAYRQNGVAYTREYFVSYPGNVMVMKLTADTPGALNRDITLDS
VQPQKSIVSDVVENTITMSGRPSDHDENGEIFTQQVKVVAKGGSVVTIGGKSCVRNADEV
LVYMTAGTNYVMRFQESDPVFSDEDPLVAVEERIAAAVKAGYDGLLKAHLEDYQALFGAM
DLNLGVTAMPDKPTDELLAAYGGRTDTPNTAEEDRYLENLYYQFGRYLLIASSREGSLPA
NLQGIWAKDLNNPWSADYHANINIQMNYWLAEQTNLSACHQPMIDYVNSLVFMGEKMAQY
YYARPDGGDVRGWAVNVGCNAWNHIASNDTEIGFVPTPAAWMCQDIWEYYQFNQDKAFLA
ENFDTLLGAALFWVDNLWTDERDGKLVVNPSYSPEHGGLSLGTTFDQGVVWEIFNEVIQA
AEVLGKTSKEIDEIKAAQSQLSGPQIGLGGQFMEWKDETTQDVTGDGGHRHVNHLFMLHP
GNQIVAGRSEQEDAYIDAMVKTLETRGDGGTGWSKAWKINFWARLRDGDHAHTMVSQLLK
ESTAENLFDLHPPFQIDGNFGGTAGMTEMLLQSQGDAIELLAALPQTWDNGTVTGLKARG
NVEVDMTWNHHTLRKSVLRPGTDGELTIRGTDLSTAKLTNAAGKSVAYTVKDKDTIVFSA
KAGESYTLQGMTDVEGTEQALQSLRDLIEETQAALDSKKPTDPMYNAKANLDLREAIDRA
DAVLQSADADKFAYLDAADALQTAYDVFLAAYDLEISLSSKSGIYTGPKKVGIRNDNPMI
SIRYTLDGSVPTAEAALYGGDILLPYGFSRLRAAAFYQGKRVGSVAAADYLVSAATNLAV
GAAAKDESNQTISGFPVSSMVDGKTDTRWATTQNGKLISTLNFNKTITFDMLTLDEFTLD
EEANRTQSYMLEYWDGTKWVEGVKSDTLDDASQMIVDNDPQHPTAHAYKAAVFPAITASK
VRLTTTGNQISIWEFGLYNTQEAGDTALLRTYTEECAALDLANYEQTQAFEAALDAAEKL
LKGAAPTQTALAAAYNALVSGRAAMIAKEKPVMIPGDVNGNGSVTAEDALLALQAATKKV
TLTDIQKRAADVDGKDGVTAADALMILQAATHKIDLGDGSGTTTSSTSSSSSSSSTKPTQ
PQPEVTKEELQAEMNRAVDTGKYTEMSVKTYLSVMETCRTVCGMEASQVTPDHYRMALEA
LREAKANLVEKPQSNWVGSFSGLGSTFGPLASEDILYADWTYADQNSIDLSAYGDRAQLR
LQFTVTFQSDDPAVNPADIWSTLVFKLRSSDTGGNENNIGWECRPADVENPARFTVSIPL
NRPGQHVSHAIDWSDINRMLLFCYLNDAYKPTAAQHSMRLSTPCIVDVTPIQETQQQIRT
ELGKTVDTSHASAAALETYRAAKQAAQTMADAPVDSVSLYDVSKALADLKNAVANLS

Enzyme Prediction     

EC	3.2.1.63

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH95	32	812	2.3e-245	0.981994459833795

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14498	Glyco_hyd_65N_2	1.27e-65	36	312	2	233	Glycosyl hydrolase family 65, N-terminal domain. This domain represents a domain found to the N-terminus of the glycosyl hydrolase 65 family catalytic domain.
cd14256	Dockerin_I	8.77e-12	1235	1288	1	54	Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.
pfam00404	Dockerin_1	8.76e-09	1236	1286	1	51	Dockerin type I repeat. The dockerin repeat is the binding partner of the cohesin domain pfam00963. The cohesin-dockerin interaction is the crucial interaction for complex formation in the cellulosome. The dockerin repeats, each bearing homology to the EF-hand calcium-binding loop bind calcium.
pfam13290	CHB_HEX_C_1	2.56e-06	941	1006	2	66	Chitobiase/beta-hexosaminidase C-terminal domain.
pfam13287	Fn3_assoc	5.61e-06	951	1010	1	59	Fn3 associated.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SCD18984.1	7.71e-277	33	852	26	816
QGT71557.1	1.46e-263	33	849	32	859
QQA09897.1	1.24e-243	33	854	35	1019
QUT72093.1	6.79e-243	33	854	35	1019
QMW88739.1	1.89e-242	33	854	35	1019

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7KMQ_A	3.67e-133	33	804	41	753	ChainA, Glyco_hyd_65N_2 domain-containing protein [Xanthomonas citri pv. citri str. 306],7KMQ_B Chain B, Glyco_hyd_65N_2 domain-containing protein [Xanthomonas citri pv. citri str. 306]
2RDY_A	1.97e-124	51	856	16	797	ChainA, BH0842 protein [Halalkalibacterium halodurans C-125],2RDY_B Chain B, BH0842 protein [Halalkalibacterium halodurans C-125]
4UFC_A	1.13e-122	33	809	22	742	Crystalstructure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus],4UFC_B Crystal structure of the GH95 enzyme BACOVA_03438 [Bacteroides ovatus]
2EAB_A	1.90e-117	36	848	21	894	Crystalstructure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAB_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum (apo form) [Bifidobacterium bifidum],2EAC_A Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum],2EAC_B Crystal structure of 1,2-a-L-fucosidase from Bifidobacterium bifidum in complex with deoxyfuconojirimycin [Bifidobacterium bifidum]
2EAD_A	1.26e-116	36	848	21	894	ChainA, Alpha-fucosidase [Bifidobacterium bifidum],2EAD_B Chain B, Alpha-fucosidase [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8L7W8	6.41e-116	52	788	66	792	Alpha-L-fucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=FUC95A PE=1 SV=1
A2R797	2.48e-106	52	826	38	789	Probable alpha-fucosidase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=afcA PE=3 SV=1
Q5AU81	2.10e-93	55	827	46	807	Alpha-fucosidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=afcA PE=1 SV=1
Q2USL3	4.79e-73	45	793	23	699	Probable alpha-fucosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=afcA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000882	0.997904	0.000290	0.000336	0.000287	0.000284

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004292_01193.