dbCAN-seq: a database of CAZyme sequence and annotation

Basic Information help

Species

Lineage

Archaea; Thermoplasmatota; Thermoplasmata; Methanomassiliicoccales; Methanomassiliicoccaceae; Methanomassiliicoccus_A;

CAZyme ID

MGYG000004312_01639

CAZy Family

GT20

CAZyme Description

Bifunctional trehalose-6-phosphate synthase/phosphatase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
466		53362.23	7.1632

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004312	1787155	MAG	Israel	Asia

Gene Location

Start: 3038; End: 4438 Strand: +

Enzyme Prediction help

EC	2.4.1.15

CAZyme Signature Domains help

Family	Start	End	Evalue	family coverage
GT20	2	461	5e-165	0.9663157894736842

CDD Domains download full data without filtering help

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd03788	GT20_TPS	0.0	3	463	1	463	trehalose-6-phosphate synthase. Trehalose-6-Phosphate Synthase (TPS, EC 2.4.1.15) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
PRK14501	PRK14501	0.0	3	463	2	461	putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional
TIGR02400	trehalose_OtsA	1.12e-169	3	458	1	450	alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., ). [Cellular processes, Adaptations to atypical conditions]
COG0380	OtsA	7.04e-167	1	460	14	475	Trehalose-6-phosphate synthase [Carbohydrate transport and metabolism].
pfam00982	Glyco_transf_20	6.31e-151	3	460	2	466	Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.

CAZyme Hits help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGN26071.1	0.0	1	465	1	465
QTL97231.1	1.07e-153	2	466	4	465
AZO94285.1	1.52e-153	2	466	4	465
CAD7768218.1	7.83e-147	4	466	14	467
CAD7768207.1	4.47e-146	4	466	14	467

PDB Hits download full data without filtering help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5HUV_A	9.76e-96	3	461	5	466	Structureof Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314],5HUV_B Structure of Candida albicans trehalose-6-phosphate synthase E341R/E346R in complex with UDP-glucose [Candida albicans SC5314]
5HUT_A	3.86e-95	3	461	5	466	Structureof Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUT_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUU_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HUU_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HVL_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314],5HVL_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314]
5JIJ_A	1.49e-90	29	458	39	470	ChainA, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5JIO_A Chain A, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5K41_A Chain A, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5K42_A Chain A, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5K44_A Chain A, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5K5C_A Chain A, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5L3K_A Chain A, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5L3K_B Chain B, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5L3K_C Chain C, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5L3K_D Chain D, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5L3K_E Chain E, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5L3K_F Chain F, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5L3K_G Chain G, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile],5L3K_H Chain H, Alpha,alpha-trehalose-phosphate synthase [Mycolicibacterium thermoresistibile]
5HVM_A	9.87e-89	3	458	13	470	Structureof Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVM_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5HXA_A	2.28e-88	1	458	9	456	Crystalstructure of an UDP-forming alpha, alpha-terhalose-phosphate synthase from Burkholderia xenovorans [Paraburkholderia xenovorans LB400]

Swiss-Prot Hits download full data without filtering help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
G4RK44	4.91e-96	3	458	2	459	Bifunctional trehalose-6-phosphate synthase/phosphatase OS=Thermoproteus tenax (strain ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1) OX=768679 GN=tpsp PE=1 SV=1
Q92410	2.11e-94	3	461	5	466	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=TPS1 PE=1 SV=1
P40387	3.16e-93	3	458	20	477	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tps1 PE=1 SV=2
A4T7Q6	3.38e-93	5	458	26	488	Trehalose-6-phosphate synthase OS=Mycolicibacterium gilvum (strain PYR-GCK) OX=350054 GN=otsA PE=3 SV=1
A0QAK7	1.08e-91	5	458	20	480	Trehalose-6-phosphate synthase OS=Mycobacterium avium (strain 104) OX=243243 GN=otsA PE=3 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000075	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM Annotations help

There is no transmembrane helices in MGYG000004312_01639.

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