CAZyme Information: MGYG000004314_00013

Basic Information

• Species: Varibaculum timonense
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Varibaculum; Varibaculum timonense
• CAZyme ID: MGYG000004314_00013
• CAZy Family: CBM32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2280	MGYG000004314_1|CGC1	245817.18	6.8023

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004314	2027678	MAG	Israel	Asia

• Gene Location: Start: 22702; End: 29544 Strand: -

Full Sequence      

MSKVSWKRRINASVLTAAVALMSVLIPASSAQALAPGDSALCADNLAVGKTVNATSREVA
GEKGPELAVDGNIGPDDKATPAIHNQSSASRWSAAGSDNNAALTIDFAGTATINKVQLYW
GNTFGKPYKLQASNDGTTWRDLVTDQNGKKAGTTEHTFNGSEKMRYLKMQISGKSQQWGL
SLWEIAACGTLAAQPTPTENENLAQLNQLIPYPQKAELAAGNSRFTLTQDSEITASGLAG
EPAKYLAETLAKSTGYKLPISASSSAKTQINYVIDPSLTVSGVGKEQMNEAYTLKIAANR
VTITARSASGALWATQSLLQLLGPWAVFPKVTVGSLMPIPAMTIQDGPRYSWRGLMIDPA
RSFIPKNEVIKLMHSMSRFKMNTLHLHLSDEGWRIDIPNEGKDATDPIDYSQLAKRGGSA
GLRAGDIDRAPQAGRTGYYTLDDYREMVATANKLGIAIVPEIDTPGHTEAALYSIPQLNS
ENSNPKPQGGQDTIGQPNYSKNNMKVNLDALNPHTYTFMQHVLKILAQNSNNTYLHIGGD
EANSMDHQHYLQYMQQILPKVKAMKQTPIVWDEALKSVGATGIADNGGVVQWWNTDSAAP
NRSQLRNDFVAKGGKFIVSNASYFYYPQREDRSHVAPSWACPGGECTLKHAYDAQPQQVM
GLSDDKGILGIEGAVWSEGVRNLADIEYIAFPRLLAHAEMGWTNQANKNWERFRGGVAQQ
ATALNVTDTSFFNGSQVTWRISPALSPIAVDSQGKGIGKANRQLIGYVSAPGKGKNNLDL
SLTLTPKESQNVINVPVSTTLDKEYIPVNGSYQTGRQMNSVFKVYADLTKVPKDSYQAQL
SAKASGLKSLDGKSDTALASAVIQVPSLSGQTPPEPGTQTSQSDTTWESTERSYPKSSYS
FPGNDGKPHKVTWDNHSLKIDGERLQVWAGEFHPWRIPSPQAWRDVLQKIKAAGFNAVSF
YFFWGDHQSEEGGPFNFDKGTRRDIDLLLTMAKEEGLYVIARPGPYVNAEISMGGLPAYM
TNYAASSLRSTDPKALAASKSWLKAFNNIAKKHLITEGGGSIIMYQVENELLTENPSTTA
FLKELSNFVRTDGITVPLFENDWGLAGRFAPNKAGNPAGKYGSTDFYTYDRYPLGFNCGA
GRGNLDDVEQTFRNYEPKGPHFIAEGQGGAFTPWGANFTPEKCASFTDGNFVRQWGTVLA
GNGVKAYTYYMIFGGTNWGYTGSPSSGFTSYDYGAGIDEDRTIRKDKFGAQKEIAYYLQA
NPQYVTSDPVAKPDIKRVSGNAQIRGYQRISSEGAAELSATGNGGRTLGFVLNNTNDTST
NKFTFALDLANTSGVGARFNHDDSDSDLKYSTGWKSVKDDSAWKGSVHEAAKAGEEVAFE
FTGTGIEIAVATGTDHGKFAVKLDDQAEQAVTGWVGTEQNKPTQKVVFSKSNLPAGKHTL
KVRVLGQSAEGSTTGGTKVQLDALNVLGASATPWIVVNNNDSAIKYNPAVGTASSWEYAT
GQNWTSGDYQGDETYSARKGDSYTYDFNGIGIDIIGAFSGNHGIADVYIDGEKVGQTAEE
VTNTARPQQVLYSVRGLSAGKHTLKVEVTGKPFAGSKGTFVSLDALRVFPDKDAIDQFNT
GTSVKDGENSWSRIPQKAGTALTLHGRDALLMSADMKIAGQKLLYTTSQLFGSPIATDNA
RVQYLLGVAGDDGETLLRYPAKPQVKAPKGVEVTWNEQYKTLRLNYKYSAEPSDITITPA
ASDRAASAGDTLRLRIIDRTYAQTTWLLNTGDGGSGKRLAVEGAELARNWSLNGSNAEVN
GTTDSKSQTPYVLLPKGVKSASWNGGKAGIGETEGVYAGFTKFIPQMLSFEPPQLKWVKQ
EGAPEKEINFDDSAWQVANGMQRNHTWQAPSANGLVLDSNYYGFYEGSVWYRATFVTSSD
SPVITLNANGGSGVPGHGKNPAFMQVWANGKSLGAASAAGAGQTFTAKGIKAGDKVVLAV
LVNNLGQNLDWSDNGLSRQNRGLYSATIPAQDGTVTWKIQGAADKSGTKDKARGLYNNGG
LYGERQGWHLPGLDDSKWTAATDMHADEAGVTWYRSTFDLNVPKDADVAFRLDVLSRRFG
NTQQTRTDHSQALLFVNGWNTGIYIGDIGPQDSFTIPAAFLNLNGKNTAAIAVIGKEADA
GPENVVLRAVHLSQMNALAPVTAQTDNPGEVEPETPDKPDTPDQPEKPNPSQPDNPQNPA
NPSPEATPTENAKTPSSKGKSVKEGLGRTGTEGVSALLIALLALGVGIGMRYRQRLQEEK

Enzyme Prediction     

No EC number prediction in MGYG000004314_00013.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH35	919	1254	3.1e-82	0.9837133550488599
GH20	345	704	1.6e-75	0.973293768545994
CBM32	54	186	7.6e-22	0.9274193548387096

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06568	GH20_SpHex_like	9.06e-91	350	714	1	326	A subgroup of the Glycosyl hydrolase family 20 (GH20) catalytic domain found in proteins similar to the N-acetylhexosaminidase from Streptomyces plicatus (SpHex). SpHex catalyzes the hydrolysis of N-acetyl-beta-hexosaminides. An Asp residue within the active site plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself. Proteins belonging to this subgroup lack the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases.
pfam01301	Glyco_hydro_35	1.20e-79	917	1255	1	315	Glycosyl hydrolases family 35.
cd06563	GH20_chitobiase-like	8.05e-79	350	715	1	355	The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. This GH20 domain family includes an N-acetylglucosamidase (GlcNAcase A) from Pseudoalteromonas piscicida and an N-acetylhexosaminidase (SpHex) from Streptomyces plicatus. SpHex lacks the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
pfam00728	Glyco_hydro_20	9.03e-73	350	704	1	345	Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.
COG3525	Chb	2.69e-60	208	726	135	637	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOZ72117.1	0.0	895	2196	54	1312
QQM67568.1	0.0	894	2196	61	1345
VEG55570.1	0.0	894	2175	62	1330
QWW19060.1	0.0	894	2171	39	1303
QGH69595.1	0.0	901	2167	67	1319

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6YXZ_JJJ	1.39e-130	30	867	4	836	ChainJJJ, Beta-N-acetylhexosaminidase [Bifidobacterium bifidum]
6Z14_JJJ	3.46e-130	30	867	4	836	ChainJJJ, Beta-N-acetylhexosaminidase [Bifidobacterium bifidum]
1HP4_A	9.32e-63	194	739	20	511	ChainA, Beta-n-acetylhexosaminidase [Streptomyces plicatus],1HP5_A Chain A, Beta-n-acetylhexosaminidase [Streptomyces plicatus],1JAK_A Chain A, Beta-N-acetylhexosaminidase [Streptomyces plicatus],1M01_A Chain A, Beta-N-acetylhexosaminidase [Streptomyces plicatus],5FCZ_A Chain A, B-N-acetylhexosaminidase [Streptomyces plicatus],5FD0_A Chain A, B-N-acetylhexosaminidase [Streptomyces plicatus]
1TG7_A	1.28e-62	911	1269	6	361	Nativestructure of beta-galactosidase from Penicillium sp. [Penicillium sp.],1XC6_A Native Structure Of Beta-Galactosidase from Penicillium sp. in complex with Galactose [Penicillium sp.]
3OG2_A	1.74e-62	911	1269	26	380	ChainA, Beta-galactosidase [Trichoderma reesei],3OGR_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGS_A Chain A, Beta-galactosidase [Trichoderma reesei],3OGV_A Chain A, Beta-galactosidase [Trichoderma reesei]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WG05	4.48e-64	911	1269	42	401	Probable beta-galactosidase E OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=lacE PE=3 SV=1
B0XXE7	4.48e-64	911	1269	42	401	Probable beta-galactosidase E OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=lacE PE=3 SV=1
A1DJ58	7.95e-64	911	1334	42	454	Probable beta-galactosidase E OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=lacE PE=3 SV=1
A1D1Z9	2.38e-63	911	1553	46	645	Probable beta-galactosidase A OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=lacA PE=3 SV=1
B0XMP7	1.33e-62	911	1334	46	452	Probable beta-galactosidase A OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=lacA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000310	0.998884	0.000186	0.000244	0.000186	0.000157

TMHMM  Annotations     

start	end
13	35
2253	2270