CAZyme Information: MGYG000004316_01336

Basic Information

• Species: Granulicatella adiacens
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Aerococcaceae; Granulicatella; Granulicatella adiacens
• CAZyme ID: MGYG000004316_01336
• CAZy Family: GH84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1720	MGYG000004316_36|CGC1	191477.17	5.4989

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004316	1849304	MAG	Israel	Asia

• Gene Location: Start: 10246; End: 15408 Strand: +

Full Sequence      

MGKRFFDKKCKYSIRKFSVGVASVMIGATFFASPVALGAEVEGEPSSENGAISEAPSLNQ
LPDEVLKAIEKAEKEGQANKAEEGASATPKEGAAASETGENQPSKEETAKPTEAEATTVA
KQAKPTEEATATEAPMTTTPNTATEATTKPREVNGTVEKADQFTADKPATKAEIDAAKKE
VTKKEYTVFPTPQKVTYGEGVTALEGKVNLVFSDSLDIYTRNRAKEILQANQVSYTTSAK
EAEGSTNIFLGVHGESSLAEKEVQDVSKDLYNKIDAYVLQVKNNKISIVGKDTDAVFFGL
TTLKHMLKDSPTPVLRDVTVEDYAEVKNRGFIEGYYGNPWTKENREDLMKYGGELKLTQY
YFAPKDDPYHNKKWRDLYPEEKLAEIRDLARVGNETKTRYVWTIHPFMHNKMRFENDEKY
KEDLSVIKNKFTQLLDAGVREFGVLADDAAWPVGGYNSYIRLMRDLTDWLTEKQKTYSGL
RKDMIFVPAWYMGQGTEDELRTLNEHLPETVHLTLTGGKVWGAVDQTFLTNLKRNLTEGG
KKYRPIHFWINWPCNDNTKQHLILGGGEKFLHPNVDLSLSNGIMLNPMQQSEASKVALFD
MAQYGWKQWRSAQEAEQINDTAFNYVVNGHFKDSEVSKAFRELGKHMRNQNRPPHVTKLE
ESVELAPKLTAFYNKLKSGQNLDAERKELKEIFAQLKADAILLKEKGDKKLIHQIHYWLD
NTVDQMNALEALLTATEGLAEKNDAKVWDNYYAGLKHYDQSISYAFFYVDHYERAEFGVQ
HIRPFINNLKEYLATHIQTMLRPDKLVTIFITNRAGAEGGLAEVTDGDYDTHVIVKTTTV
IERDDYVGLRFNKAIKIHTLGFATGTKTADNYTFGNAVVEYQNEQGDWVALTTPAYTGRE
RTLEFNNLDITAQAVRMRATAKKDNAWLAMREIAVNRPLETGSKKVKANITLSSNLVYKY
GTSVVQMQDENATTESMMAHANQTNVTPENAWVQMDLGAAKAIKRVHLVQGERDKLAAGV
IEYSTDGTNWTTLQTLAGERTSDIIKEFTAQFVRVRNTQTLNKWWRIADFTVDVFTPNTD
LTVTSVDSLKETPVVDSRGRYEMTLPQGTNIPANGYLGMKLDRLHEASSITLEDAGETGL
TLEYSANEVEWYRADELPEHTFVRYVRLVNKTNKDQALPTGKLIVKTKEVEPTELQSTTM
GIHQYYGANDVRRIHNISQLFDGDYNNFVEFSDYQRKNGEIVMKLGTTRQVKKIRAYIQD
AERNYLRDGKIQVSEDGKNWTDVVTVGDGVENEIRDNSLTDGWTHDAAKPGNRYIEGVLD
KPVTAKFMRVLFTADYNARFLGFSEIEINDGEFINPENNPTVTGSGTEDKDNLKKHLTDG
NVLTSYATTENKGELVYHLSEETAVNHVRLIADVPEGTTVKVKVRTLNEAGESVWKELGK
VKSSFQTFALSGENPRILDVKVSWEGGPAEFYELSTFTKEVTPDEIQQPEPDKPLTPLTP
IDSTVAKRELKDEATGVMISGSTTALEPVKDLAVQKVLDQTLDGKKYDAYDIRLGGKAGG
SVQPNGTVAVTVPVKADAEVETIYYITPEKRLEALAFTQKDGKVTFNTTHFSVYAVVYKG
SVAVANPTAPTTPTAPTSPTSPVTPGTPTTPSTPPAVAFGEPGRTTPAGEKPVAKPETVN
GAVANQLPATGTSDANTSLFVASLALALGTLFYKKGKEEA

Enzyme Prediction     

EC	3.2.1.35	3.2.1.52

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	329	643	2.6e-94	0.9898305084745763

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	2.45e-100	329	643	1	293	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam02838	Glyco_hydro_20b	1.25e-18	185	322	1	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.
TIGR01168	YSIRK_signal	2.28e-12	3	40	2	39	Gram-positive signal peptide, YSIRK family. Many surface proteins found in Streptococcus, Staphylococcus, and related lineages share apparently homologous signal sequences. A motif resembling [YF]SIRKxxxGxxS[VIA] appears at the start of the transmembrane domain. The GxxS motif appears perfectly conserved, suggesting a specific function and not just homology. There is a strong correlation between proteins carrying this region at the N-terminus and those carrying the Gram-positive anchor domain with the LPXTG sortase processing site at the C-terminus.
pfam04650	YSIRK_signal	9.18e-10	8	31	1	24	YSIRK type signal peptide. Many surface proteins found in Streptococcus, Staphylococcus, and related lineages share apparently homologous signal sequences. A motif resembling [YF]SIRKxxxGxxS[VIA] appears at the start of the transmembrane domain. The GxxS motif appears perfectly conserved, suggesting a specific function and not just homology. There is a strong correlation between proteins carrying this region at the N-terminus and those carrying the Gram-positive anchor domain with the LPXTG sortase processing site at the C-terminus.
NF038031	PavB_Nterm	1.21e-08	11	162	11	116	PavB family adhesin N-terminal domain. This HMM describes the portion of PavB from Streptococcus pneumoniae, and closely related proteins from Streptococcus mitis and Streptococcus pseudopneumoniae, N-terminal to the repetitive region with variable numbers of SSURE (Streptococcal Surface REpeats) regions (see PF11966), which bind fibronectin. The PavB region is notable, in part, for its rare variant, WSIRR, of the YSIRK motif signal peptide. Full-length versions of proteins from this family have a C-terminal LPXTG-containing region for sortase-mediated anchoring to the cell wall.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AQA08903.1	0.0	1	1494	1	1492
QQQ35904.1	0.0	1	1494	1	1492
QUB38718.1	0.0	154	1619	1218	2830
AMD97395.1	0.0	154	1491	1222	2559
AMH89096.1	0.0	154	1491	1218	2555

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PV4_A	7.79e-187	181	801	27	652	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
6PWI_A	1.76e-113	179	794	27	625	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6PV5_A	1.42e-60	184	756	38	595	Structureof CpGH84B [Clostridium perfringens ATCC 13124]
5MI4_A	5.27e-54	190	770	18	553	BtGH84mutant with covalent modification by MA3 [Bacteroides thetaiotaomicron VPI-5482],5MI5_A BtGH84 mutant with covalent modification by MA3 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482],5MI6_A BtGH84 mutant with covalent modification by MA3 in complex with Thiamet G [Bacteroides thetaiotaomicron VPI-5482],5MI7_A BtGH84 mutant with covalent modification by MA4 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482]
2J4G_A	7.99e-54	190	770	6	541	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482],2J4G_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	4.82e-271	181	1480	34	1354	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q89ZI2	5.96e-53	190	770	28	563	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
Q0TR53	1.87e-48	190	723	48	559	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
Q8XL08	3.28e-48	190	723	48	559	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
O60502	1.29e-19	330	602	63	329	Protein O-GlcNAcase OS=Homo sapiens OX=9606 GN=OGA PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000609	0.998394	0.000228	0.000275	0.000241	0.000196

TMHMM  Annotations     

start	end
17	39