CAZyme Information: MGYG000004317_00198

Basic Information

• Species: Lachnospira sp900316325
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnospira; Lachnospira sp900316325
• CAZyme ID: MGYG000004317_00198
• CAZy Family: CE8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2110		229036.59	4.6685

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004317	2482422	MAG	Israel	Asia

• Gene Location: Start: 40434; End: 46766 Strand: +

Full Sequence      

MKRIAKRRLSCLLSAILLLVMSLDMSAFAGVFEKTARKIDVWDFGGVAESNTEMYQNNIS
ASDWDNCSDVGTNAKFNAASIKFGDLTLITTTGDRLYSTSSKNYGASGYATTKYADGYAA
NGMFYSNGTGGESRRCFTIDNVKAGDKIVVYMVSSNAATSNLIFKYLGESGKQQDSKEFS
NTAGKYEFIAKYDGQYKVYADAVAGKPCYNRVVRIPGVNVSGTIDLNGADIKDYKIVFKD
EANDISYEAEVKNGSFNVVLAAGCDYTAALSGVTGYGFTSDTKVISTKTDDVISGKDGVI
LKVEAKSVYKYTGKLTGFDKNYDISKLSVKLEADKNTLADDITLNLNSDMTFEATLEPDV
EYTAVLYGVNDYEIVKGATILDNKDVSSDIEVALKKVYKATGKFNGLDDASKVTAIKFVN
VEDKYEYTGTVKDGGYEVSLRDGSYQVVTTVADYRTTTHVVVKGSDVSKDILFVSTKKAD
ALTRVPDIYVGYEDKAELNYNTVKEAVAAAAAMNPSKEDERVTIHIKPGTYREQVVVTTP
YISFVNDEKNSGKDVLLTWYYGIGYEYYSIDSTGFYNEENAYDKYEKAQASKWGCSVYVK
NSATAFRADGITFEASFNRYITDEEIEDGVNAANTKLPQRTYGLDATSKAATERATALAI
EADKTEFTNCAFLGSQDTLYTGNSATNLYFKNCRIEGNTDYIFGDGNAVFDGCELRFFGY
STGSVGGYITAHKPGTATQYGYLFRNCTITGNSDLTVKAGYLGRPWGADARVTFINTKMT
DNLINDAGWYEMAVKPEKANFAEYNTRLTTGEAVDLSKRAGKALSDSDAAAINVKSYFGS
WTPVSYKDENGTVEFKTVPFVVDNGDINAPYPGHTLTAGYSLGDVNDASDASVIRWCRVD
KDGNETLVKSSTANVDKTYKITKNDIGNYIKVVVVPETLSGNKAAEKSYKVEAFVRDGFE
DPSGSTDIELGDGVNIFLAGDSTVKDYSAAGMYMSGKAQSEGAWGEFLQSFFDSSKVKVQ
NYANGGRSSRNFINEGSLDKIKQNIKEGDYLFIQFGHNDCANGTGYLEDRYVPLGTPDAD
GVYPSTAGTKTATPSSLVSKYGDTFYSYNCGGTYKWYLQQYIDAAKSVGAIPVLVTPVSR
LYYNSDGTIKPHHDSTDKTTGTYVSSNDAYVTAVKQLASEQNVLLLDGFAITKSLYEETY
KNDSSAKSGVSQLATQIMAAGDKTHSNKLGGFITAALFASKLQDMNLSISKAVSMPAKTA
GINPDGQQIFSINGSSVFTAYAADDNGKYSAQSEYWTNYGQELIKAIGTKKDELNKTPDP
VPDPTPTPDPTPTPDPDPTPAPSAKATVWVVGDSTVSSFSDKYYYPRYGWGTQLSAYFDD
TLTVNNLALSGRSSKSYTSEPQYKTLIEGMQNGDYLLIGFGHNDEKAEEARYTNPNGDYK
TAGSFANSLYENYIKPAQDKGVTVVVCTPIVRRTATKDWANSNLHITSASGAFEGGDYAK
SIINLGKDTGVAVVDMTSLTKQLYDELGPDETVNLHAWTSSKSSSVDNTHTNIWGGRYNA
YLITKTIKELGVKGISSHIIDAEAPKKEDVLKKNPDYKEAEYVGVVKDSTIWSPAGVFKG
TVFGNVGGAPSKSNQTLEMNDNKFHIQVKNNKGKIGSSADGVAMFYYKVPVKSKFTITAN
ATVNSFDLNDQVSFGLMARDDMYIDENRTDVLGDYVAAGPLKLATAGGVWNCFARKSGVL
TQGGTCKSEVKAGQTYALKIESNSDGYACTFGDEDTITGGFDFMLTTVDSEYVYVGMFVA
RNADVTFSDVTLIVDGEVIAGNVPTPDPTPDPTPDPTPDPTPDPTPTPDPTPDPTPDPTP
TPDPKPENPGSMVVDDVIEKTEGVDKAVVKVSSDAVKVTEEEKALIESGKDLHISAEIKN
AKDTVTKKQKELIDSEVKKYAENGITGLYLDIKMTKKIGDGYKAPVSELSTPATFSVKIA
DELKNTDSSKERTYSVVRIHGEKAEVIDSTFDEASSTLTFATDRFSVYAVVYEDKTKSLE
ITKPDNENTDNNVDKPSSSETVTPDDSQNTNAAPETGDSFGKDIMAASFAMIICAMGAAY
VLMTRKRRQF

Enzyme Prediction     

EC	3.-.-.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CE8	496	836	1.5e-61	0.9583333333333334
CE12	975	1242	9.3e-59	0.9952380952380953
CE12	1347	1567	7.6e-54	0.9952380952380953

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01821	Rhamnogalacturan_acetylesterase_like	8.18e-50	1347	1567	2	198	Rhamnogalacturan_acetylesterase_like subgroup of SGNH-hydrolases. Rhamnogalacturan acetylesterase removes acetyl esters from rhamnogalacturonan substrates, and renders them susceptible to degradation by rhamnogalacturonases. Rhamnogalacturonans are highly branched regions in pectic polysaccharides, consisting of repeating -(1,2)-L-Rha-(1,4)-D-GalUA disaccharide units, with many rhamnose residues substituted by neutral oligosaccharides such as arabinans, galactans and arabinogalactans. Extracellular enzymes participating in the degradation of plant cell wall polymers, such as Rhamnogalacturonan acetylesterase, would typically be found in saprophytic and plant pathogenic fungi and bacteria.
cd01821	Rhamnogalacturan_acetylesterase_like	1.09e-48	975	1239	2	195	Rhamnogalacturan_acetylesterase_like subgroup of SGNH-hydrolases. Rhamnogalacturan acetylesterase removes acetyl esters from rhamnogalacturonan substrates, and renders them susceptible to degradation by rhamnogalacturonases. Rhamnogalacturonans are highly branched regions in pectic polysaccharides, consisting of repeating -(1,2)-L-Rha-(1,4)-D-GalUA disaccharide units, with many rhamnose residues substituted by neutral oligosaccharides such as arabinans, galactans and arabinogalactans. Extracellular enzymes participating in the degradation of plant cell wall polymers, such as Rhamnogalacturonan acetylesterase, would typically be found in saprophytic and plant pathogenic fungi and bacteria.
PLN02432	PLN02432	2.30e-33	515	841	34	291	putative pectinesterase
PLN02773	PLN02773	3.86e-32	499	830	16	288	pectinesterase
PLN02682	PLN02682	5.00e-31	515	794	93	323	pectinesterase family protein

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADD61757.1	0.0	18	1322	1	1306
QAV09174.1	0.0	36	1825	38	1895
QSI02582.1	0.0	6	1316	2	1275
ADL50681.1	4.31e-213	1346	1816	45	516
ADL52345.1	6.87e-198	1	884	12	917

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2NSP_A	1.19e-20	500	843	18	339	ChainA, Pectinesterase A [Dickeya dadantii 3937],2NSP_B Chain B, Pectinesterase A [Dickeya dadantii 3937],2NST_A Chain A, Pectinesterase A [Dickeya dadantii 3937],2NST_B Chain B, Pectinesterase A [Dickeya dadantii 3937],2NT6_A Chain A, Pectinesterase A [Dickeya dadantii 3937],2NT6_B Chain B, Pectinesterase A [Dickeya dadantii 3937],2NT9_A Chain A, Pectinesterase A [Dickeya dadantii 3937],2NT9_B Chain B, Pectinesterase A [Dickeya dadantii 3937]
5C1C_A	4.65e-20	596	846	67	299	CrystalStructure of the Pectin Methylesterase from Aspergillus niger in Deglycosylated Form [Aspergillus niger ATCC 1015]
5C1E_A	4.65e-20	596	846	67	299	CrystalStructure of the Pectin Methylesterase from Aspergillus niger in Penultimately Deglycosylated Form (N-acetylglucosamine Stub at Asn84) [Aspergillus niger ATCC 1015]
1QJV_A	8.47e-17	500	843	18	339	ChainA, PECTIN METHYLESTERASE [Dickeya chrysanthemi],1QJV_B Chain B, PECTIN METHYLESTERASE [Dickeya chrysanthemi]
2NTB_A	2.74e-16	500	843	18	339	Crystalstructure of pectin methylesterase in complex with hexasaccharide V [Dickeya dadantii 3937],2NTB_B Crystal structure of pectin methylesterase in complex with hexasaccharide V [Dickeya dadantii 3937],2NTP_A Crystal structure of pectin methylesterase in complex with hexasaccharide VI [Dickeya dadantii 3937],2NTP_B Crystal structure of pectin methylesterase in complex with hexasaccharide VI [Dickeya dadantii 3937],2NTQ_A Crystal structure of pectin methylesterase in complex with hexasaccharide VII [Dickeya dadantii 3937],2NTQ_B Crystal structure of pectin methylesterase in complex with hexasaccharide VII [Dickeya dadantii 3937]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31528	2.89e-25	975	1253	4	213	Probable rhamnogalacturonan acetylesterase YesY OS=Bacillus subtilis (strain 168) OX=224308 GN=yesY PE=1 SV=1
A1DBT4	5.41e-25	654	846	142	324	Probable pectinesterase A OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=pmeA PE=3 SV=1
Q9FM79	2.69e-24	507	807	95	342	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=1 SV=1
Q4WBT5	4.94e-23	654	846	142	324	Probable pectinesterase A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=pmeA PE=3 SV=1
B0Y9F9	4.94e-23	654	846	142	324	Probable pectinesterase A OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=pmeA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001243	0.997915	0.000192	0.000242	0.000178	0.000176

TMHMM  Annotations     

start	end
9	31
2084	2103