CAZyme Information: MGYG000004317_00225

Basic Information

• Species: Lachnospira sp900316325
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnospira; Lachnospira sp900316325
• CAZyme ID: MGYG000004317_00225
• CAZy Family: GH13
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
794	MGYG000004317_2|CGC1	90202.6	4.8945

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004317	2482422	MAG	Israel	Asia

• Gene Location: Start: 78575; End: 80959 Strand: +

Full Sequence      

MGLNMGNRLYDLMDWPSIEGVVYSECDEPMELLGGRICKDGFLIQTFAPDAVEMNVRVDG
RKKLYPMEKMDEAGFFAVLLPGRKKVSYTFVKEDIYGKKTEYKDIYSYGRQECMHALLKI
SQNGRSEYNINKFMTGSAQDAYKYMGSHEAVVDGISGMIFGIWAPDARRVSVIGAFNKWD
GRVFQMEKDYDTGIFFLFIPDMKSKTPYCYEIKLKDGQVVKKADPYAQSASNDVIYASLA
GVNPEEKNFKWTDAGWKQEDTKHKPLSICEIDILDLKDDTIEDRIEALGFNCVEIMNVCA
VRNGSSAETINFYAVNCEVGGLRLKAFIDKIHKKNISVIIDWNGAFMSQGEYSLVYFDGT
HLYDTGKVNLDNHPELNVASFDYSKPQVRSFLMSNIRYWIEEYHVDGLKFSETASMLYLD
YGRKPGEWIPNIYGGKENINAVEFIQNVRKMVDSLQKNTILAAEESSGWQFVTGDIADGG
LGFDYKWNDGWKKELVPFIEMDPLFRKGIYGRLTYSMLYQYSDDFIIGFSRNGYGWKKGI
LKDIVPVNGEDVLNHVKTALGYMYFHPGKKLINIKECSGVEKFVAQMNNLYRNTKALYEC
DDNPSGFEWADDISAEETVIAVLRRDESGQSVIGAVNFTPVERKEFCIGVDEAGKYTDVL
NDSVVYESSEHNWNSKDESVFVDLKPLGISLYTYEPYTEIEKERIKIEKETKEALMLAKK
EADEAKAVKDAAIERAKQAKEAEIIALAAAKEAEKAEKEAAKKAEEARIACIKIDENAKK
KLAELKKMEEKQGE

Enzyme Prediction     

No EC number prediction in MGYG000004317_00225.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	283	574	9.7e-79	0.9767441860465116

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK05402	PRK05402	5.05e-167	18	696	6	725	1,4-alpha-glucan branching protein GlgB.
PRK12313	PRK12313	7.81e-149	126	696	8	629	1,4-alpha-glucan branching protein GlgB.
COG0296	GlgB	8.81e-125	133	694	14	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14705	PRK14705	1.46e-107	152	692	637	1220	glycogen branching enzyme; Provisional
PRK12568	PRK12568	1.26e-104	16	669	13	692	glycogen branching enzyme; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACR71776.1	2.08e-180	5	718	1	725
QNM06418.1	6.65e-155	5	714	1	761
AOZ96491.1	4.50e-154	5	719	1	743
QNM03882.1	3.73e-150	5	719	1	766
ASN98288.1	1.48e-149	5	702	1	752

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQZ_A	4.82e-104	6	706	18	785	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	3.57e-103	6	706	18	785	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	3.57e-103	6	706	18	785	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQW_A	3.57e-103	6	706	18	785	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
6KLF_A	5.63e-103	18	696	6	751	ChainA, 1,4-alpha-glucan branching enzyme GlgB [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q3J3M6	7.33e-112	3	693	1	726	1,4-alpha-glucan branching enzyme GlgB OS=Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) OX=272943 GN=glgB PE=3 SV=1
A3PIQ5	2.82e-111	3	693	1	726	1,4-alpha-glucan branching enzyme GlgB OS=Cereibacter sphaeroides (strain ATCC 17029 / ATH 2.4.9) OX=349101 GN=glgB PE=3 SV=1
Q21M30	4.06e-111	18	692	9	725	1,4-alpha-glucan branching enzyme GlgB OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) OX=203122 GN=glgB PE=3 SV=1
Q1AZ86	3.59e-110	17	688	6	713	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
Q2W2Q6	1.64e-108	28	695	33	739	1,4-alpha-glucan branching enzyme GlgB OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000068	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004317_00225.