CAZyme Information: MGYG000004329_01776

Basic Information

• Species: Prevotellamassilia sp900539625
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotellamassilia; Prevotellamassilia sp900539625
• CAZyme ID: MGYG000004329_01776
• CAZy Family: GH33
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
400		43790.61	8.5777

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004329	2571737	MAG	Israel	Asia

• Gene Location: Start: 2568; End: 3770 Strand: -

Full Sequence      

MRILSIFAAFLASTLIASAQTCLFHTGDSTGYPYRIPAIAKAKNGQLIALSDLRPCGGDI
GYGRVDILSRVSADNGASWSEAVPVLQGSGKGADAGYGDACLVADRDRNELLLVCVSGDM
PYWQSSPERRQRIVATHARYNKKNGKWEWSPLPPFDLTKQVYEDIFDGRIRGLFMGSGRI
CQSRMVKVGKYYRLYGALCTHRGNFVIYSDDFGRTWNALGSAEVSCAPSGDEPKCEELPD
GSVLLSSRRSGGRFFNVFRYTDTAKAKGVWGQPADTRFAPGGIKNESGACNGEILIVPCH
RADGKATTLALQSLPAGPGRSNVTIYWKELVAAADYNTPLRFASNWQGAFRVTSLGSAYS
TMVQQADGRIAFFYEEEPQYYQMIYKAIDLSEITGGKFVK

Enzyme Prediction     

No EC number prediction in MGYG000004329_01776.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH33	21	385	5.6e-45	0.9649122807017544

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd15482	Sialidase_non-viral	1.39e-62	23	389	3	339	Non-viral sialidases. Sialidases or neuraminidases function to bind and hydrolyze terminal sialic acid residues from various glycoconjugates, they play vital roles in pathogenesis, bacterial nutrition and cellular interactions. They have a six-bladed, beta-propeller fold with the non-viral sialidases containing 2-5 Asp-box motifs (most commonly Ser/Thr-X-Asp-[X]-Gly-X-Thr- Trp/Phe). This CD includes eubacterial and eukaryotic sialidases.
COG4409	NanH	3.50e-04	18	130	258	390	Neuraminidase (sialidase) [Carbohydrate transport and metabolism, Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFQ12979.1	5.15e-194	9	398	9	397
EFC70822.1	1.32e-146	11	398	11	402
QYR10380.1	2.14e-109	33	398	36	395
ALO48969.1	3.04e-108	23	398	29	399
QUB70796.1	4.37e-84	33	398	48	428

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1EUR_A	3.29e-13	31	375	21	344	Sialidase[Micromonospora viridifaciens],1EUS_A Sialidase Complexed With 2-Deoxy-2,3-Dehydro-N- Acetylneuraminic Acid [Micromonospora viridifaciens]
1EUT_A	6.11e-13	31	375	21	344	Sialidase,Large 68kd Form, Complexed With Galactose [Micromonospora viridifaciens],1EUU_A Sialidase Or Neuraminidase, Large 68kd Form [Micromonospora viridifaciens]
7MHU_A	9.86e-13	16	296	32	302	ChainA, Exo-alpha-sialidase [Bacteroides acidifaciens],7MHU_B Chain B, Exo-alpha-sialidase [Bacteroides acidifaciens]
1W8N_A	1.43e-12	31	375	17	340	Contributionof the Active Site Aspartic Acid to Catalysis in the Bacterial Neuraminidase from Micromonospora viridifaciens. [Micromonospora viridifaciens],1W8O_A Contribution of the Active Site Aspartic Acid to Catalysis in the Bacterial Neuraminidase from Micromonospora viridifaciens [Micromonospora viridifaciens]
1WCQ_A	1.43e-12	31	253	17	238	Mutagenesisof the Nucleophilic Tyrosine in a Bacterial Sialidase to Phenylalanine. [Micromonospora viridifaciens],1WCQ_B Mutagenesis of the Nucleophilic Tyrosine in a Bacterial Sialidase to Phenylalanine. [Micromonospora viridifaciens],1WCQ_C Mutagenesis of the Nucleophilic Tyrosine in a Bacterial Sialidase to Phenylalanine. [Micromonospora viridifaciens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q02834	3.49e-12	31	375	63	386	Sialidase OS=Micromonospora viridifaciens OX=1881 GN=nedA PE=1 SV=1
P29767	7.25e-06	23	375	384	809	Sialidase OS=Clostridium septicum OX=1504 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000368	0.998887	0.000228	0.000164	0.000154	0.000153

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004329_01776.